Table 1.

Boltzmann fit parameters for the hSlo1+hβ1 complexes with human-to-Drosophila mutations

Segment Mutation Control 3 µM DHA  
  V0.5 Qapp V0.5 Qapp n 
  mV  mV   
S5 V236A 110.8 ± 1.1 1.10 ± 0.05 62.8 ± 2.7 1.10 ± 0.11 6 
S5 N237Q 208.8 ± 0.8 0.95 ± 0.03 162.2 ± 1.3 0.87 ± 0.04 6 
S5 L239V 144.3 ± 1.3 1.02 ± 0.05 93.6 ± 2.4 0.91 ± 0.07 8 
S5 T245V 130.5 ± 1.0 1.13 ± 0.04 79.7 ± 1.4 1.14 ± 0.06 8 
S5 F252I 235.7 ± 1.0 0.81 ± 0.04 184.2 ± 1.2 0.66 ± 0.02 6 
P/filter L280F 99.6 ± 1.9 0.90 ± 0.06 51.0 ± 1.8 0.89 ± 0.05 6 
P/filter M282I 290.4 ± 6.5 0.52 ± 0.07 195.4 ± 1.8 0.74 ± 0.04 7 
P/filter A295C 187.4 ± 1.2 0.84 ± 0.04 129.4 ± 2.3 0.84 ± 0.06 6 
P/filter K296E 163.3 ± 1.1 0.88 ± 0.03 110.8 ± 1.9 0.86 ± 0.05 6 
P/filter T298V 163.9 ± 1.1 0.90 ± 0.03 112.4 ± 1.5 0.92 ± 0.04 6 
S6 L302T 146.8 ± 2.3 0.85 ± 0.06 92.9 ± 2.5 0.87 ± 0.07 6 
S6 M304L 175.6 ± 1.5 0.75 ± 0.03 127.7 ± 1.8 0.78 ± 0.04 6 
S6 I308L:G310V 155.2 ± 1.3 0.84 ± 0.03 117.1 ± 2.8 0.85 ± 0.06 8 
S6 Y318S 159.1 ± 1.2 0.79 ± 0.03 152.4 ± 1.2 0.75 ± 0.03 14 
S6 V319I 195.4 ± 0.9 0.90 ± 0.03 147.4 ± 1.6 0.79 ± 0.04 8 
S6 I326V 184.7 ± 1.5 0.78 ± 0.04 129.9 ± 3.0 0.76 ± 0.06 6 

All results were obtained in the absence of intracellular Ca2+. In each construct, the values estimated from the individual experiments were pooled and fit with the Boltzmann function.

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