Table 1. Boltzmann fit parameters for... | Rockefeller University Press

Table 1.

Boltzmann fit parameters for the hSlo1+hβ1 complexes with human-to-Drosophila mutations

Segment	Mutation	Control		3 µM DHA
		V_0.5	Q_app	V_0.5	Q_app	n
		mV		mV
S5	V236A	110.8 ± 1.1	1.10 ± 0.05	62.8 ± 2.7	1.10 ± 0.11	6
S5	N237Q	208.8 ± 0.8	0.95 ± 0.03	162.2 ± 1.3	0.87 ± 0.04	6
S5	L239V	144.3 ± 1.3	1.02 ± 0.05	93.6 ± 2.4	0.91 ± 0.07	8
S5	T245V	130.5 ± 1.0	1.13 ± 0.04	79.7 ± 1.4	1.14 ± 0.06	8
S5	F252I	235.7 ± 1.0	0.81 ± 0.04	184.2 ± 1.2	0.66 ± 0.02	6
P/filter	L280F	99.6 ± 1.9	0.90 ± 0.06	51.0 ± 1.8	0.89 ± 0.05	6
P/filter	M282I	290.4 ± 6.5	0.52 ± 0.07	195.4 ± 1.8	0.74 ± 0.04	7
P/filter	A295C	187.4 ± 1.2	0.84 ± 0.04	129.4 ± 2.3	0.84 ± 0.06	6
P/filter	K296E	163.3 ± 1.1	0.88 ± 0.03	110.8 ± 1.9	0.86 ± 0.05	6
P/filter	T298V	163.9 ± 1.1	0.90 ± 0.03	112.4 ± 1.5	0.92 ± 0.04	6
S6	L302T	146.8 ± 2.3	0.85 ± 0.06	92.9 ± 2.5	0.87 ± 0.07	6
S6	M304L	175.6 ± 1.5	0.75 ± 0.03	127.7 ± 1.8	0.78 ± 0.04	6
S6	I308L:G310V	155.2 ± 1.3	0.84 ± 0.03	117.1 ± 2.8	0.85 ± 0.06	8
S6	Y318S	159.1 ± 1.2	0.79 ± 0.03	152.4 ± 1.2	0.75 ± 0.03	14
S6	V319I	195.4 ± 0.9	0.90 ± 0.03	147.4 ± 1.6	0.79 ± 0.04	8
S6	I326V	184.7 ± 1.5	0.78 ± 0.04	129.9 ± 3.0	0.76 ± 0.06	6

Segment	Mutation	Control		3 µM DHA
		V_0.5	Q_app	V_0.5	Q_app	n
		mV		mV
S5	V236A	110.8 ± 1.1	1.10 ± 0.05	62.8 ± 2.7	1.10 ± 0.11	6
S5	N237Q	208.8 ± 0.8	0.95 ± 0.03	162.2 ± 1.3	0.87 ± 0.04	6
S5	L239V	144.3 ± 1.3	1.02 ± 0.05	93.6 ± 2.4	0.91 ± 0.07	8
S5	T245V	130.5 ± 1.0	1.13 ± 0.04	79.7 ± 1.4	1.14 ± 0.06	8
S5	F252I	235.7 ± 1.0	0.81 ± 0.04	184.2 ± 1.2	0.66 ± 0.02	6
P/filter	L280F	99.6 ± 1.9	0.90 ± 0.06	51.0 ± 1.8	0.89 ± 0.05	6
P/filter	M282I	290.4 ± 6.5	0.52 ± 0.07	195.4 ± 1.8	0.74 ± 0.04	7
P/filter	A295C	187.4 ± 1.2	0.84 ± 0.04	129.4 ± 2.3	0.84 ± 0.06	6
P/filter	K296E	163.3 ± 1.1	0.88 ± 0.03	110.8 ± 1.9	0.86 ± 0.05	6
P/filter	T298V	163.9 ± 1.1	0.90 ± 0.03	112.4 ± 1.5	0.92 ± 0.04	6
S6	L302T	146.8 ± 2.3	0.85 ± 0.06	92.9 ± 2.5	0.87 ± 0.07	6
S6	M304L	175.6 ± 1.5	0.75 ± 0.03	127.7 ± 1.8	0.78 ± 0.04	6
S6	I308L:G310V	155.2 ± 1.3	0.84 ± 0.03	117.1 ± 2.8	0.85 ± 0.06	8
S6	Y318S	159.1 ± 1.2	0.79 ± 0.03	152.4 ± 1.2	0.75 ± 0.03	14
S6	V319I	195.4 ± 0.9	0.90 ± 0.03	147.4 ± 1.6	0.79 ± 0.04	8
S6	I326V	184.7 ± 1.5	0.78 ± 0.04	129.9 ± 3.0	0.76 ± 0.06	6

All results were obtained in the absence of intracellular Ca²⁺. In each construct, the values estimated from the individual experiments were pooled and fit with the Boltzmann function.

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