Table 1.
Primers used for qRT-PCR reactions
NameUseSequence
GAPDH Reference ER F 5′-ATG​ACA​TCA​TCA​AGA​AGG​TGG​TG-3′ 
R 5′-CAT​ACC​AGG​AAA​TGA​GCT​TG-3′ 
HPRT Reference F 5′-TTT​ACT​GGC​AAC​ATC​AAC​AG-3′ 
R 5′-CAG​ATC​CCA​CAT​ACT​CAC​TG-3′ 
RPS29 Reference F 5′-ACG​GTC​TGA​TCC​GCA​AAT​AC-3′ 
R 5′-CAT​GAT​CGG​TTC​CAC​TTG​GT-3′ 
Pou2f3 Tuft cell gene F 5′-AGA​GAA​TCA​ACT​GCC​CCG​TG-3′ 
R 5′-GGA​AGG​CAC​GAC​TCT​CTT​CC-3′ 
DCLK1 Tuft cell gene F 5′-CAG​CCT​GGA​CGA​GCT​GGT​GG-3′ 
R 5′-TGA​CCA​GTT​GGG​GTT​CAC​AT-3′ 
Muc2 Goblet cell gene F 5′-CAG​TTT​ATT​CCT​GTG​TGC​CCA​AGG-3′ 
R 5′-GGC​TTC​AGA​ATA​ATG​TAC​TGC​TGC-3′ 
Lysozyme Paneth cell gene F 5′-GGA​ATG​GAT​GGC​TAC​CGT​GG-3′ 
R 5′-CAT​GCC​ACC​CAT​GCT​CGA​AT-3′ 
Sca1 Stem cell gene F 5′-GAT​GGA​CAC​TTC​TCA​CAC​TAC​A-3′ 
R 5′-GCA​GGT​AAT​TGA​TGG​GCA​AGA-3′ 
Lgr5 Stem cell gene F 5′-CTC​CAA​CCT​CAG​CGT​CTT​C-3′ 
R 5′-GTC​AAA​GCA​TTT​CCA​GCA​AGA-3′ 
Atoh1 Cell fate gene F 5′-AGC​TTC​CTC​TGG​GGG​TTA​CT-3′ 
R 5′-TTC​TGT​GCC​ATC​ATC​GCT​GT-3′ 
Neurog3 Cell fate gene F 5′-CTC​AGC​AAA​CAG​CGA​AGA​AG-3′ 
R 5′-GGG​AAG​GTG​GGC​AGG​AC-3′ 
Hes1 Cell fate gene F 5′-AGT​GTC​ACC​TTC​CAG​TGG​CT-3′ 
R 5′-TGG​GCT​AGG​GAC​TTT​ACG​GG-3′ 
NameUseSequence
GAPDH Reference ER F 5′-ATG​ACA​TCA​TCA​AGA​AGG​TGG​TG-3′ 
R 5′-CAT​ACC​AGG​AAA​TGA​GCT​TG-3′ 
HPRT Reference F 5′-TTT​ACT​GGC​AAC​ATC​AAC​AG-3′ 
R 5′-CAG​ATC​CCA​CAT​ACT​CAC​TG-3′ 
RPS29 Reference F 5′-ACG​GTC​TGA​TCC​GCA​AAT​AC-3′ 
R 5′-CAT​GAT​CGG​TTC​CAC​TTG​GT-3′ 
Pou2f3 Tuft cell gene F 5′-AGA​GAA​TCA​ACT​GCC​CCG​TG-3′ 
R 5′-GGA​AGG​CAC​GAC​TCT​CTT​CC-3′ 
DCLK1 Tuft cell gene F 5′-CAG​CCT​GGA​CGA​GCT​GGT​GG-3′ 
R 5′-TGA​CCA​GTT​GGG​GTT​CAC​AT-3′ 
Muc2 Goblet cell gene F 5′-CAG​TTT​ATT​CCT​GTG​TGC​CCA​AGG-3′ 
R 5′-GGC​TTC​AGA​ATA​ATG​TAC​TGC​TGC-3′ 
Lysozyme Paneth cell gene F 5′-GGA​ATG​GAT​GGC​TAC​CGT​GG-3′ 
R 5′-CAT​GCC​ACC​CAT​GCT​CGA​AT-3′ 
Sca1 Stem cell gene F 5′-GAT​GGA​CAC​TTC​TCA​CAC​TAC​A-3′ 
R 5′-GCA​GGT​AAT​TGA​TGG​GCA​AGA-3′ 
Lgr5 Stem cell gene F 5′-CTC​CAA​CCT​CAG​CGT​CTT​C-3′ 
R 5′-GTC​AAA​GCA​TTT​CCA​GCA​AGA-3′ 
Atoh1 Cell fate gene F 5′-AGC​TTC​CTC​TGG​GGG​TTA​CT-3′ 
R 5′-TTC​TGT​GCC​ATC​ATC​GCT​GT-3′ 
Neurog3 Cell fate gene F 5′-CTC​AGC​AAA​CAG​CGA​AGA​AG-3′ 
R 5′-GGG​AAG​GTG​GGC​AGG​AC-3′ 
Hes1 Cell fate gene F 5′-AGT​GTC​ACC​TTC​CAG​TGG​CT-3′ 
R 5′-TGG​GCT​AGG​GAC​TTT​ACG​GG-3′ 
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