Table 1. The effect of SMase D on hNa V... | Rockefeller University Press

Table 1.

The effect of SMase D on hNa_V inactivation kinetics in Xenopus oocytes^a

Parameters	hNa_V1.4α	hNa_V1.4α + β1	hNa_V1.5α	hNa_V1.5α + β1	^muthNa_V1.4α^b
Control
τ	7.8 ± 0.2 ms^c	1.0 ± 0.1 ms^c	1.1 ± 0.04 ms^c	1.3 ± 0.02 ms^c	2.2 ± 0.1 ms^c
SMase D
τ	9.5 ± 0.2 ms^c				7.0 ± 0.2 ms^c
τ₁		0.7 ± 0.2 ms^d	0.5 ± 0.2 ms^d	0.6 ± 0.1 ms^d
τ₂		7.0 ± 0.2 ms^d	3.8 ± 0.2 ms^d	5.6 ± 0.2 ms^d
A₁/(A₁ + A₂)		0.32 ± 0.01^d	0.34 ± 0.01^d	0.47 ± 0.02^d
A₂/(A₁ + A₂)		0.68 ± 0.01^d	0.66 ± 0.01^d	0.53 ± 0.02^d

Parameters	hNa_V1.4α	hNa_V1.4α + β1	hNa_V1.5α	hNa_V1.5α + β1	^muthNa_V1.4α^b
Control
τ	7.8 ± 0.2 ms^c	1.0 ± 0.1 ms^c	1.1 ± 0.04 ms^c	1.3 ± 0.02 ms^c	2.2 ± 0.1 ms^c
SMase D
τ	9.5 ± 0.2 ms^c				7.0 ± 0.2 ms^c
τ₁		0.7 ± 0.2 ms^d	0.5 ± 0.2 ms^d	0.6 ± 0.1 ms^d
τ₂		7.0 ± 0.2 ms^d	3.8 ± 0.2 ms^d	5.6 ± 0.2 ms^d
A₁/(A₁ + A₂)		0.32 ± 0.01^d	0.34 ± 0.01^d	0.47 ± 0.02^d
A₂/(A₁ + A₂)		0.68 ± 0.01^d	0.66 ± 0.01^d	0.53 ± 0.02^d

Data is presented as mean ± SEM (n = 4 – 8).

Contains the S5–S6 linkers of hNa_V1.5.

Inactivation time courses were fit to $I (t) = A e^{t / τ} .$

Inactivation time courses were fit to $I (t) = A_{1} e^{t / τ_{1}} + A_{2} e^{t / τ_{2}} .$