Fitted parameters of QON-V from R217 mutants
| Mutant | V1/2 | zQ | n |
| mV | |||
| C363S | 57 ± 0.4 | 1.56 ± 0.03 | 5 |
| R217Q-C363S | −16 ± 0.5 | 1.07 ± 0.02 | 3 |
| R217E-C363S | −67 ± 0.9 | 1.32 ± 0.06 | 5 |
| R217H-C363S pH 6.1 | −13 ± 3 | 0.89 ± 0.03 | 5 |
| 58 ± 1 | 1.60 ± 0.08 | ||
| R217H-C363S pH 7.4 | −14 ± 6 | 1.0 ± 0.04 | 5 |
| 28 ± 3 | 2.0 ± 0.6 |
| Mutant | V1/2 | zQ | n |
| mV | |||
| C363S | 57 ± 0.4 | 1.56 ± 0.03 | 5 |
| R217Q-C363S | −16 ± 0.5 | 1.07 ± 0.02 | 3 |
| R217E-C363S | −67 ± 0.9 | 1.32 ± 0.06 | 5 |
| R217H-C363S pH 6.1 | −13 ± 3 | 0.89 ± 0.03 | 5 |
| 58 ± 1 | 1.60 ± 0.08 | ||
| R217H-C363S pH 7.4 | −14 ± 6 | 1.0 ± 0.04 | 5 |
| 28 ± 3 | 2.0 ± 0.6 |
When two values of V1/2 and zQ are given, the fit corresponds to a sum of two two-state Boltzmann functions.