Comparison of the effects of mutations at positions 537 and 538 on closed- and open-state stability
| 537 | Parameter | 538 | ||
| R | K | Q | ||
| R | C | WT | O | |
| ΔG0 | 2.7 | −8.4 (2 s), −10.5 (10 s) | −26.5 | |
| ΔΔG0 | 13.2 | −18.1 | ||
| ΔΔG0,NA | ||||
| K | C | C | O | |
| ΔG0 | −0.9 | −2.6 | −17.1 | |
| ΔΔG0 | 9.5 | 5.8 | −8.7 | |
| ΔΔG0,NA | 11.5 | 3.6 | ||
| Q | C | NA | O | |
| ΔG0 | −3.0 | −4.5 | −17.7 | |
| ΔΔG0 | 7.4 | 3.9 | −9.3 | |
| ΔΔG0,NA | 11.7 | 4.9 |
| 537 | Parameter | 538 | ||
| R | K | Q | ||
| R | C | WT | O | |
| ΔG0 | 2.7 | −8.4 (2 s), −10.5 (10 s) | −26.5 | |
| ΔΔG0 | 13.2 | −18.1 | ||
| ΔΔG0,NA | ||||
| K | C | C | O | |
| ΔG0 | −0.9 | −2.6 | −17.1 | |
| ΔΔG0 | 9.5 | 5.8 | −8.7 | |
| ΔΔG0,NA | 11.5 | 3.6 | ||
| Q | C | NA | O | |
| ΔG0 | −3.0 | −4.5 | −17.7 | |
| ΔΔG0 | 7.4 | 3.9 | −9.3 | |
| ΔΔG0,NA | 11.7 | 4.9 |
Letters (C/O) within cells indicate whether the combination of residues at 537 (rows) and 538 (columns) resulted in a stabilization of the closed or open state, relative to WT hERG. For ease of comparison, the mean values for ΔG0, ΔΔG0, and ΔΔG0,NA (in kJ mol−1) are also shown. Refer to Table 1 for full details, including n values and SEM.