Table 2. Kinetic parameters for... | Rockefeller University Press

Table 2.

Kinetic parameters for activation (10–90% time to peak current, 20 mV), time constants of entry (20 mV), and recovery from fast inactivation (−100 mV), and delay in onset to recovery (−100 mV)

Mutation	Location	Time to peak activation	Fast inactivation tau	Recovery tau	Recovery delay
		ms	ms	ms	ms
hNa_V1.4		1.05 ± 0.03	1.26 ± 0.03	7.15 ± 0.54	1.04 ± 0.05
ENC
S151K	Domain I S1	1.33 ± 0.03^d	1.23 ± 0.05	11.3 ± 0.61^d	2.18 ± 0.16^d
D152R	Domain I S1	1.04 ± 0.04	1.15 ± 0.06	8.09 ± 0.48	1.42 ± 0.19^a
E161R	Domain I S2	1.18 ± 0.03^b	1.31 ± 0.07	5.34 ± 0.25^b	0.95 ± 0.06
E598K	Domain II S1	1.09 ± 0.07	2.13 ± 0.27^b	5.17 ± 0.48^a	1.09 ± 0.10
N614K	Domain II S2	1.15 ± 0.06	1.65 ± 0.10^b	5.50 ± 0.38^a	0.86 ± 0.05^a
E1051R	Domain III S1	1.55 ± 0.14^b	3.25 ± 0.55^b	3.61 ± 0.24^d	0.79 ± 0.07^b
E1051D	Domain III S1	1.05 ± 0.04	1.23 ± 0.06	5.51 ± 0.30^a	1.17 ± 0.10
D1069K	Domain III S2	1.18 ± 0.09	2.92 ± 0.46^b	3.42 ± 0.16^d	0.84 ± 0.05^b
D1069E	Domain III S2	1.18 ± 0.05^a	1.94 ± 0.16^b	6.31 ± 0.36	1.15 ± 0.09
E1373K	Domain IV S1	1.65 ± 0.07^d	5.51 ± 0.31^d	3.42 ± 0.39^d	0.44 ± 0.07^d
E1373N	Domain IV S1	1.54 ± 0.07^d	4.94 ± 0.18^d	3.70 ± 0.23^d	0.46 ± 0.02^d
E1373D	Domain IV S1	1.24 ± 0.03^d	2.74 ± 0.22^d	4.85 ± 0.22^c	0.81 ± 0.05^b
N1389K	Domain IV S2	1.85 ± 0.12^c	4.14 ± 0.29^d	4.31 ± 0.42^c	0.42 ± 0.02^d
N1389E	Domain IV S2	1.26 ± 0.04^d	1.79 ± 0.07^d	8.36 ± 0.55	1.25 ± 0.07^a
N1389D	Domain IV S2	1.21 ± 0.02^d	1.52 ± 0.04^d	10.66 ± 0.39^d	1.21 ± 0.10
HCR
N144K	Domain I S1	1.65 ± 0.11^d	2.51 ± 0.30^c	6.19 ± 0.37	1.46 ± 0.48^c
N144R	Domain I S1	1.54 ± 0.02^d	1.70 ± 0.08^d	7.63 ± 0.30	0.94 ± 0.25
N144D	Domain I S1	1.02 ± 0.01	1.11 ± 0.04^c	9.60 ± 1.1^a	1.39 ± 0.11^c
N144E	Domain I S1	1.05 ± 0.02	1.15 ± 0.04^a	7.19 ± 0.60	1.09 ± 0.05
N591K	Domain II S1	1.56 ± 0.09^d	2.62 ± 0.21^d	5.68 ± 0.31^a	0.97 ± 0.06
N591R	Domain II S1	1.43 ± 0.02^d	1.33 ± 0.04	5.51 ± 0.28^a	0.88 ± 0.07
N591D	Domain II S1	1.25 ± 0.03^d	1.09 ± 0.03^c	6.90 ± 0.43	1.00 ± 0.06
N591E	Domain II S1	1.15 ± 0.04^a	1.11 ± 0.03^b	7.90 ± 0.59	1.53 ± 0.12^c
S1044K	Domain III S1	1.20 ± 0.03^b	1.63 ± 0.17^a	6.46 ± 0.41	1.14 ± 0.04
S1044R	Domain III S1	1.30 ± 0.03^d	1.55 ± 0.03^d	4.49 ± 0.25^c	0.67 ± 0.03^d
S1044D	Domain III S1	1.23 ± 0.04^c	1.84 ± 0.1^d	9.94 ± 0.33^d	1.96 ± 0.13^d
S1044N	Domain III S1	1.10 ± 0.03	1.27 ± 0.04	5.82 ± 0.33^a	0.95 ± 0.07
N1366K	Domain IV S1	1.17 ± 0.03^b	1.83 ± 0.10^d	5.89 ± 0.38	0.94 ± 0.05
N1366R	Domain IV S1	1.91 ± 0.07^d	4.09 ± 0.16^d	14.62 ± 0.58^d	0.40 ± 0.07^d
N1366D	Domain IV S1	1.09 ± 0.03	1.60 ± 0.03^d	13.02 ± 0.88^d	2.99 ± 0.13^d
N1366E	Domain IV S1	1.37 ± 0.03^d	2.05 ± 0.08^d	6.45 ± 0.35	1.37 ± 0.06^c
INC
E171R	Domain I S2	1.09 ± 0.04	1.41 ± 0.06^a	9.59 ± 0.48^b	2.20 ± 0.21^d
K175E	Domain I S2	1.13 ± 0.02^a	1.45 ± 0.09	6.21 ± 0.36	1.05 ± 0.06
N194K	Domain I S3	1.52 ± 0.04^d	4.64 ± 0.15^d	6.18 ± 0.30	1.13 ± 0.04
D197R	Domain I S3	1.02 ± 0.04	1.56 ± 0.08^b	16.1 ± 1.43^d	2.80 ± 0.37^d
E624K	Domain II S2	1.26 ± 0.04^d	1.57 ± 0.09^b	6.11 ± 0.28	0.91 ± 0.08
K628E	Domain II S2	1.12 ± 0.03	1.45 ± 0.07^a	6.72 ± 0.15	1.09 ± 0.04
N643K	Domain II S3	1.24 ± 0.04^c	1.49 ± 0.05^c	6.04 ± 0.37	0.96 ± 0.11
D646K	Domain II S3	1.50 ± 0.04^d	2.09 ± 0.32^a	7.13 ± 0.30	1.05 ± 0.09
E1079R	Domain III S2	1.54 ± 0.05^d	2.24 ± 0.18^d	7.13 ± 0.36	1.07 ± 0.09
K1083E	Domain III S2	1.16 ± 0.04^a	1.31 ± 0.05	8.22 ± 0.33	2.18 ± 0.25^d
C1098K	Domain III S3	1.11 ± 0.02	1.36 ± 0.04^a	10.4 ± 0.60^c	1.61 ± 0.11^c
D1101V	Domain III S3	1.09 ± 0.02	1.23 ± 0.07	7.63 ± 0.38	1.26 ± 0.05^b
E1399K	Domain IV S2	1.57 ± 0.04^d	3.25 ± 0.08^d	7.75 ± 0.91	1.21 ± 0.12
K1403E	Domain IV S2	1.19 ± 0.06	2.47 ± 0.30^b	5.44 ± 0.22	1.10 ± 0.06
N1417K	Domain IV S3	1.23 ± 0.03^d	1.57 ± 0.06^d	7.50 ± 0.32	1.25 ± 0.08^a
D1420K	Domain IV S3	1.45 ± 0.05^d	3.52 ± 0.48^c	7.32 ± 0.31	0.80 ± 0.11^a

Mutation	Location	Time to peak activation	Fast inactivation tau	Recovery tau	Recovery delay
		ms	ms	ms	ms
hNa_V1.4		1.05 ± 0.03	1.26 ± 0.03	7.15 ± 0.54	1.04 ± 0.05
ENC
S151K	Domain I S1	1.33 ± 0.03^d	1.23 ± 0.05	11.3 ± 0.61^d	2.18 ± 0.16^d
D152R	Domain I S1	1.04 ± 0.04	1.15 ± 0.06	8.09 ± 0.48	1.42 ± 0.19^a
E161R	Domain I S2	1.18 ± 0.03^b	1.31 ± 0.07	5.34 ± 0.25^b	0.95 ± 0.06
E598K	Domain II S1	1.09 ± 0.07	2.13 ± 0.27^b	5.17 ± 0.48^a	1.09 ± 0.10
N614K	Domain II S2	1.15 ± 0.06	1.65 ± 0.10^b	5.50 ± 0.38^a	0.86 ± 0.05^a
E1051R	Domain III S1	1.55 ± 0.14^b	3.25 ± 0.55^b	3.61 ± 0.24^d	0.79 ± 0.07^b
E1051D	Domain III S1	1.05 ± 0.04	1.23 ± 0.06	5.51 ± 0.30^a	1.17 ± 0.10
D1069K	Domain III S2	1.18 ± 0.09	2.92 ± 0.46^b	3.42 ± 0.16^d	0.84 ± 0.05^b
D1069E	Domain III S2	1.18 ± 0.05^a	1.94 ± 0.16^b	6.31 ± 0.36	1.15 ± 0.09
E1373K	Domain IV S1	1.65 ± 0.07^d	5.51 ± 0.31^d	3.42 ± 0.39^d	0.44 ± 0.07^d
E1373N	Domain IV S1	1.54 ± 0.07^d	4.94 ± 0.18^d	3.70 ± 0.23^d	0.46 ± 0.02^d
E1373D	Domain IV S1	1.24 ± 0.03^d	2.74 ± 0.22^d	4.85 ± 0.22^c	0.81 ± 0.05^b
N1389K	Domain IV S2	1.85 ± 0.12^c	4.14 ± 0.29^d	4.31 ± 0.42^c	0.42 ± 0.02^d
N1389E	Domain IV S2	1.26 ± 0.04^d	1.79 ± 0.07^d	8.36 ± 0.55	1.25 ± 0.07^a
N1389D	Domain IV S2	1.21 ± 0.02^d	1.52 ± 0.04^d	10.66 ± 0.39^d	1.21 ± 0.10
HCR
N144K	Domain I S1	1.65 ± 0.11^d	2.51 ± 0.30^c	6.19 ± 0.37	1.46 ± 0.48^c
N144R	Domain I S1	1.54 ± 0.02^d	1.70 ± 0.08^d	7.63 ± 0.30	0.94 ± 0.25
N144D	Domain I S1	1.02 ± 0.01	1.11 ± 0.04^c	9.60 ± 1.1^a	1.39 ± 0.11^c
N144E	Domain I S1	1.05 ± 0.02	1.15 ± 0.04^a	7.19 ± 0.60	1.09 ± 0.05
N591K	Domain II S1	1.56 ± 0.09^d	2.62 ± 0.21^d	5.68 ± 0.31^a	0.97 ± 0.06
N591R	Domain II S1	1.43 ± 0.02^d	1.33 ± 0.04	5.51 ± 0.28^a	0.88 ± 0.07
N591D	Domain II S1	1.25 ± 0.03^d	1.09 ± 0.03^c	6.90 ± 0.43	1.00 ± 0.06
N591E	Domain II S1	1.15 ± 0.04^a	1.11 ± 0.03^b	7.90 ± 0.59	1.53 ± 0.12^c
S1044K	Domain III S1	1.20 ± 0.03^b	1.63 ± 0.17^a	6.46 ± 0.41	1.14 ± 0.04
S1044R	Domain III S1	1.30 ± 0.03^d	1.55 ± 0.03^d	4.49 ± 0.25^c	0.67 ± 0.03^d
S1044D	Domain III S1	1.23 ± 0.04^c	1.84 ± 0.1^d	9.94 ± 0.33^d	1.96 ± 0.13^d
S1044N	Domain III S1	1.10 ± 0.03	1.27 ± 0.04	5.82 ± 0.33^a	0.95 ± 0.07
N1366K	Domain IV S1	1.17 ± 0.03^b	1.83 ± 0.10^d	5.89 ± 0.38	0.94 ± 0.05
N1366R	Domain IV S1	1.91 ± 0.07^d	4.09 ± 0.16^d	14.62 ± 0.58^d	0.40 ± 0.07^d
N1366D	Domain IV S1	1.09 ± 0.03	1.60 ± 0.03^d	13.02 ± 0.88^d	2.99 ± 0.13^d
N1366E	Domain IV S1	1.37 ± 0.03^d	2.05 ± 0.08^d	6.45 ± 0.35	1.37 ± 0.06^c
INC
E171R	Domain I S2	1.09 ± 0.04	1.41 ± 0.06^a	9.59 ± 0.48^b	2.20 ± 0.21^d
K175E	Domain I S2	1.13 ± 0.02^a	1.45 ± 0.09	6.21 ± 0.36	1.05 ± 0.06
N194K	Domain I S3	1.52 ± 0.04^d	4.64 ± 0.15^d	6.18 ± 0.30	1.13 ± 0.04
D197R	Domain I S3	1.02 ± 0.04	1.56 ± 0.08^b	16.1 ± 1.43^d	2.80 ± 0.37^d
E624K	Domain II S2	1.26 ± 0.04^d	1.57 ± 0.09^b	6.11 ± 0.28	0.91 ± 0.08
K628E	Domain II S2	1.12 ± 0.03	1.45 ± 0.07^a	6.72 ± 0.15	1.09 ± 0.04
N643K	Domain II S3	1.24 ± 0.04^c	1.49 ± 0.05^c	6.04 ± 0.37	0.96 ± 0.11
D646K	Domain II S3	1.50 ± 0.04^d	2.09 ± 0.32^a	7.13 ± 0.30	1.05 ± 0.09
E1079R	Domain III S2	1.54 ± 0.05^d	2.24 ± 0.18^d	7.13 ± 0.36	1.07 ± 0.09
K1083E	Domain III S2	1.16 ± 0.04^a	1.31 ± 0.05	8.22 ± 0.33	2.18 ± 0.25^d
C1098K	Domain III S3	1.11 ± 0.02	1.36 ± 0.04^a	10.4 ± 0.60^c	1.61 ± 0.11^c
D1101V	Domain III S3	1.09 ± 0.02	1.23 ± 0.07	7.63 ± 0.38	1.26 ± 0.05^b
E1399K	Domain IV S2	1.57 ± 0.04^d	3.25 ± 0.08^d	7.75 ± 0.91	1.21 ± 0.12
K1403E	Domain IV S2	1.19 ± 0.06	2.47 ± 0.30^b	5.44 ± 0.22	1.10 ± 0.06
N1417K	Domain IV S3	1.23 ± 0.03^d	1.57 ± 0.06^d	7.50 ± 0.32	1.25 ± 0.08^a
D1420K	Domain IV S3	1.45 ± 0.05^d	3.52 ± 0.48^c	7.32 ± 0.31	0.80 ± 0.11^a

Mean values ± SEM from 10–24 experiments.

P ≤ 0.05.

P ≤ 0.01.

P ≤ 0.001.

P ≤ 0.0001.

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