Table 2.

Mutant cycle analysis of rates obtained from Ca2+-dependent deactivation

D50-K61 ΔΔG coupling 
 kcal 
Koff (K61RD50N; slow) −0.38 
Koff (K61RD50N; fast) −0.99 
Kon (K61RD50N; slow) −0.46 
Kon (K61RD50N; fast) −1.1 
D50-K61 ΔΔG coupling 
 kcal 
Koff (K61RD50N; slow) −0.38 
Koff (K61RD50N; fast) −0.99 
Kon (K61RD50N; slow) −0.46 
Kon (K61RD50N; fast) −1.1 
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