Table 3. Recovery fractional magnitudes... | Rockefeller University Press

Table 3.

Recovery fractional magnitudes of recovery from SI

Channel variant (measured parameter)	Pulse	A_F	A_I	A_S	C	n
	s
WT (I)	5	0.10 ± 0.02 (0.89)	0.00 ± 0.00 (0.05)	0.01 ± 0.01 (0.06)	0.89 ± 0.03	4
WT (I)	40	0.13 ± 0.02 (0.32)^a	0.15 ± 0.04 (0.37)^a	0.12 ± 0.01 (0.31)^a	0.60 ± 0.03	4
WT (I)	160	0.07 ± 0.03 (0.10)^a^b	0.35 ± 0.02 (0.50)^a	0.28 ± 0.02 (0.41)^a	0.30 ± 0.05	4
WT (Q)	5	0.10 ± 0.02 (0.73)	0.03 ± 0.02 (0.25)	0.00 ± 0.00 (0.02)	0.86 ± 0.03	5
WT (Q)	40	0.12 ± 0.02 (0.48)^a	0.12 ± 0.02 (0.51)^a	0.03 ± 0.03 (0.01)	0.76 ± 0.03	5
WT (Q)	160	0.15 ± 0.03 (0.39)^a	0.18 ± 0.07 (0.48)^a	0.05 ± 0.01 (0.13)^b	0.62 ± 0.08	5
DI (F)	5	0.17 ± 0.04 (0.98)	0.00 ± 0.00 (0.02)	0.00 ± 0.00 (0.00)	0.83 ± 0.04	7
DI (F)	40	0.22 ± 0.05 (0.53)^a	0.19 ± 0.04 (0.46)^a	0.00 ± 0.00 (0.01)	0.59 ± 0.04	7
DI (F)	160	0.13 ± 0.03 (0.35)^a	0.18 ± 0.03 (0.46)^a	0.08 ± 0.03 (0.20)^a^b	0.61 ± 0.04	7
DII (F)	5	0.20 ± 0.08 (0.94)	0.01 ± 0.01 (0.06)	0.00 ± 0.00 (0.00)	0.79 ± 0.07	3
DII (F)	40	0.12 ± 0.10 (0.36)	0.21 ± 0.02 (0.61)	0.01 ± 0.01 (0.03)	0.66 ± 0.09	3
DII (F)	160	0.13 ± 0.11 (0.28)	0.22 ± 0.07 (0.49)	0.11 ± 0.03 (0.23)^a^b	0.54 ± 0.06	3
DIII (F)	5	0.34 ± 0.08 (0.89)	0.04 ± 0.02 (0.09)	0.01 ± 0.01 (0.02)	0.61 ± 0.06	6
DIII (F)	40	0.16 ± 0.08 (0.32)^a	0.18 ± 0.05 (0.36)	0.17 ± 0.03 (0.32)^a	0.48 ± 0.06	6
DIII (F)	160	0.04 ± 0.04 (0.07)^a	0.23 ± 0.01 (0.38)^a	0.32 ± 0.03 (0.55)^a^b	0.41 ± 0.02	6
DIV (F)	5	0.08 ± 0.05 (0.72)	0.03 ± 0.01 (0.28)	0.00 ± 0.00 (0.00)	0.89 ± 0.05	7
DIV (F)	40	0.03 ± 0.02 (0.12)^a	0.14 ± 0.02 (0.59)	0.07 ± 0.03 (0.29)^a	0.76 ± 0.04	7
DIV (F)	160	0.07 ± 0.03 (0.19)^a	0.11 ± 0.02 (0.31)^b	0.18 ± 0.03 (0.50)^a^b	0.65 ± 0.05	7

Channel variant (measured parameter)	Pulse	A_F	A_I	A_S	C	n
	s
WT (I)	5	0.10 ± 0.02 (0.89)	0.00 ± 0.00 (0.05)	0.01 ± 0.01 (0.06)	0.89 ± 0.03	4
WT (I)	40	0.13 ± 0.02 (0.32)^a	0.15 ± 0.04 (0.37)^a	0.12 ± 0.01 (0.31)^a	0.60 ± 0.03	4
WT (I)	160	0.07 ± 0.03 (0.10)^a^b	0.35 ± 0.02 (0.50)^a	0.28 ± 0.02 (0.41)^a	0.30 ± 0.05	4
WT (Q)	5	0.10 ± 0.02 (0.73)	0.03 ± 0.02 (0.25)	0.00 ± 0.00 (0.02)	0.86 ± 0.03	5
WT (Q)	40	0.12 ± 0.02 (0.48)^a	0.12 ± 0.02 (0.51)^a	0.03 ± 0.03 (0.01)	0.76 ± 0.03	5
WT (Q)	160	0.15 ± 0.03 (0.39)^a	0.18 ± 0.07 (0.48)^a	0.05 ± 0.01 (0.13)^b	0.62 ± 0.08	5
DI (F)	5	0.17 ± 0.04 (0.98)	0.00 ± 0.00 (0.02)	0.00 ± 0.00 (0.00)	0.83 ± 0.04	7
DI (F)	40	0.22 ± 0.05 (0.53)^a	0.19 ± 0.04 (0.46)^a	0.00 ± 0.00 (0.01)	0.59 ± 0.04	7
DI (F)	160	0.13 ± 0.03 (0.35)^a	0.18 ± 0.03 (0.46)^a	0.08 ± 0.03 (0.20)^a^b	0.61 ± 0.04	7
DII (F)	5	0.20 ± 0.08 (0.94)	0.01 ± 0.01 (0.06)	0.00 ± 0.00 (0.00)	0.79 ± 0.07	3
DII (F)	40	0.12 ± 0.10 (0.36)	0.21 ± 0.02 (0.61)	0.01 ± 0.01 (0.03)	0.66 ± 0.09	3
DII (F)	160	0.13 ± 0.11 (0.28)	0.22 ± 0.07 (0.49)	0.11 ± 0.03 (0.23)^a^b	0.54 ± 0.06	3
DIII (F)	5	0.34 ± 0.08 (0.89)	0.04 ± 0.02 (0.09)	0.01 ± 0.01 (0.02)	0.61 ± 0.06	6
DIII (F)	40	0.16 ± 0.08 (0.32)^a	0.18 ± 0.05 (0.36)	0.17 ± 0.03 (0.32)^a	0.48 ± 0.06	6
DIII (F)	160	0.04 ± 0.04 (0.07)^a	0.23 ± 0.01 (0.38)^a	0.32 ± 0.03 (0.55)^a^b	0.41 ± 0.02	6
DIV (F)	5	0.08 ± 0.05 (0.72)	0.03 ± 0.01 (0.28)	0.00 ± 0.00 (0.00)	0.89 ± 0.05	7
DIV (F)	40	0.03 ± 0.02 (0.12)^a	0.14 ± 0.02 (0.59)	0.07 ± 0.03 (0.29)^a	0.76 ± 0.04	7
DIV (F)	160	0.07 ± 0.03 (0.19)^a	0.11 ± 0.02 (0.31)^b	0.18 ± 0.03 (0.50)^a^b	0.65 ± 0.05	7

Similar to SI onset, recovery from SI can be fit with three time constants, τ_F = 0.6 ± 0.3 s, τ_I = 4.5 ± 1.1 s, and τ_S = 43.1 ± 4.8 s, which were found by fitting the recovery of the wild-type ionic current after a 160-s depolarizing pulse to +45. The fractional magnitudes below are for pulses to +45 mV lasting 5, 40, and 160 s. The absolute values correlate to the fraction inactivated during onset, which are described in Tables 1 and 2. To compute significance differences in time constants between durations, magnitudes were normalized to the fraction inactivated (shown in parentheses). For example, after the 5-s pulse, the fraction of current inactivated with τ_F was 0.91. p-values were computed to compare fraction inactivated with a given τ. I, the ionic current recovery in Fig. 6 A; Q, the gating current recovery in Fig. 6 B; DI, DII, DIII, and DIV, the fluorescence recoveries in Fig. 7 B. Channel variant abbreviations as in Table 1.

Significantly different (P < 0.05) from the 5-s pulse.

Significantly different from 40 s (160 pulses).

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