Table 2.

Cl− and Na+ permeabilities obtained from GHK fits to instantaneous current-voltage relationships of 15 HEK293 cells

Cell type NMDG-Cl in bath NaCl in bath PNa/PCl PCl−NMDG-Cl/PCl−NaCl 
 PCl ±SD PCl ±SD PNa ±SD   
 10−14 cm3/s/pF 10−14 cm3/s/pF 10−14 cm3/s/pF 10−14 cm3/s/pF 10−14 cm3/s/pF 10−14 cm3/s/pF   
WT 30.4 1.9 29.6 1.2 7.1 0.6 0.24 1.03 
WT 12.9 0.6 31.7 1.0 9.7 0.5 0.31 0.41 
I342A 26.8 0.8 55.6 2.0 14.7 1.7 0.26 0.48 
I342A 19.8 0.8 93.8 4.2 13.3 3.6 0.14 0.21 
I342A 138.5 15.0 230.2 1.6 77.9 1.4 0.34 0.60 
I342A 87.2 6.9 127.6 2.4 67.7 1.9 0.53 0.68 
K616E 204.1 16.0 176.8 12.7 45.8 10.6 0.26 1.15 
K616E 45.7 4.7 137.7 7.7 30.6 6.4 0.22 0.33 
K616E 12.8 1.0 23.4 2.5 12.7 2.1 0.54 0.55 
R592E 10.6 3.4 12.7 2.3 9.7 2.0 0.76 0.83 
R592E 42.4 1.3 72.7 3.5 11.2 3.0 0.15 0.58 
R636E 16.2 1.7 27.3 1.8 3.1 1.5 0.11 0.59 
R636E 90.0 2.4 130.4 1.2 69.5 1.0 0.53 0.69 
R636E 16.9 1.5 22.9 1.7 8.0 1.4 0.35 0.74 
R636E 150.0 8.5 232.0 4.6 79.9 3.8 0.34 0.65 

As explained in Eq. 4 (compare with Fig. 7, A–C). The error of each estimate is indicated by the SD of the fit given by the Origin fitting routine (OriginLab).

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