| Surface/ spots name . | Source channel . | Smoothing: surface details (μm) . | Background subtraction: diameter of largest sphere (μm) . | Absolute intensity threshold (μm) . | Split touching objects: split seed diameter (μm) . | Quality threshold . | Voxel number threshold . |
|---|---|---|---|---|---|---|---|
| MHCII surfaces | MHCII AF555 | 0.5 | 15 | 8.3 | 10 | 6.3 | 1,500 |
| S. Tm surfaces | S. Tm. AF647 | 0.5 | - | 4.3 | - | - | - |
| Surface/ spots name . | Source channel . | Smoothing: surface details (μm) . | Background subtraction: diameter of largest sphere (μm) . | Absolute intensity threshold (μm) . | Split touching objects: split seed diameter (μm) . | Quality threshold . | Voxel number threshold . |
|---|---|---|---|---|---|---|---|
| MHCII surfaces | MHCII AF555 | 0.5 | 15 | 8.3 | 10 | 6.3 | 1,500 |
| S. Tm surfaces | S. Tm. AF647 | 0.5 | - | 4.3 | - | - | - |