Recovery fractional magnitudes of recovery from SI
| Channel variant (measured parameter) | Pulse | AF | AI | AS | C | n |
| s | ||||||
| L689I (I) | 5 | 0.21 ± 0.06 (1.00) | 0.00 ± 0.00 (0.00) | 0.00 ± 0.00 (0.00) | 0.79 ± 0.06 | 3 |
| L689I (I) | 40 | 0.16 ± 0.06 (0.70)a | 0.07 ± 0.04 (0.30) | 0.00 ± 0.00 (0.00)a | 0.77 ± 0.09 | 3 |
| L689I (I) | 160 | 0.07 ± 0.01 (0.21) | 0.13 ± 0.05 (0.35) | 0.16 ± 0.09 (0.45) | 0.64 ± 0.08 | 3 |
| L689I (Q) | 5 | 0.02 ± 0.01 (0.52) | 0.01 ± 0.00 (0.24) | 0.01 ± 0.01 (0.24) | 0.96 ± 0.01 | 4 |
| L689I (Q) | 40 | 0.03 ± 0.01 (0.34) | 0.03 ± 0.03 (0.35) | 0.03 ± 0.02 (0.32) | 0.92 ± 0.04 | 4 |
| L689I (Q) | 160 | 0.11 ± 0.08 (0.43) | 0.09 ± 0.06 (0.37) | 0.05 ± 0.02 (0.20) | 0.76 ± 0.08 | 4 |
| L689I DI (F) | 5 | 0.15 ± 0.03 (0.84) | 0.01 ± 0.01 (0.05) | 0.02 ± 0.02 (0.11) | 0.81 ± 0.02 | 3 |
| L689I DI (F) | 40 | 0.18 ± 0.01 (0.80) | 0.00 ± 0.00 (0.00)a | 0.05 ± 0.03 (0.20)a | 0.78 ± 0.03 | 3 |
| L689I DI (F) | 160 | 0.11 ± 0.29 (0.35) | 0.08 ± 0.03 (0.27) | 0.12 ± 0.06 (0.38) | 0.69 ± 0.05 | 3 |
| L689I DII (F) | 5 | 0.09 ± 0.01 (0.89) | 0.01 ± 0.01 (0.11) | 0.00 ± 0.00 (0.00) | 0.90 ± 0.02 | 3 |
| L689I DII (F) | 40 | 0.15 ± 0.07 (0.63) | 0.04 ± 0.02 (0.19) | 0.04 ± 0.02 (0.18) | 0.77 ± 0.03 | 3 |
| L689I DII (F) | 160 | 0.21 ± 0.09 (0.70) | 0.00 ± 0.00 (0.00) | 0.09 ± 0.05 (0.31) | 0.69 ± 0.05 | 3 |
| L689I DIII (F) | 5 | 0.38 ± 0.10 (0.82) | 0.02 ± 0.02 (0.04) | 0.06 ± 0.04 (0.14) | 0.54 ± 0.09 | 4 |
| L689I DIII (F) | 40 | 0.30 ± 0.14 (0.49) | 0.13 ± 0.10 (0.21) | 0.19 ± 0.05 (0.30) | 0.38 ± 0.15 | 4 |
| L689I DIII (F) | 160 | 0.04 ± 0.04 (0.06) | 0.31 ± 0.11 (0.42) | 0.39 ± 0.10 (0.53) | 0.26 ± 0.06 | 4 |
| L689I DIV (F) | 5 | 0.12 ± 0.06 (0.57) | 0.01 ± 0.01 (0.04) | 0.08 ± 0.05 (0.39) | 0.79 ± 0.06 | 5 |
| L689I DIV (F) | 40 | 0.25 ± 0.07 (0.75)a | 0.07 ± 0.04 (0.22)a | 0.01 ± 0.01 (0.03) | 0.67 ± 0.12 | 5 |
| L689I DIV (F) | 160 | 0.13 ± 0.05 (0.38) | 0.04 ± 0.02 (0.12) | 0.16 ± 0.08 (0.39) | 0.68 ± 0.07 | 5 |
| Channel variant (measured parameter) | Pulse | AF | AI | AS | C | n |
| s | ||||||
| L689I (I) | 5 | 0.21 ± 0.06 (1.00) | 0.00 ± 0.00 (0.00) | 0.00 ± 0.00 (0.00) | 0.79 ± 0.06 | 3 |
| L689I (I) | 40 | 0.16 ± 0.06 (0.70)a | 0.07 ± 0.04 (0.30) | 0.00 ± 0.00 (0.00)a | 0.77 ± 0.09 | 3 |
| L689I (I) | 160 | 0.07 ± 0.01 (0.21) | 0.13 ± 0.05 (0.35) | 0.16 ± 0.09 (0.45) | 0.64 ± 0.08 | 3 |
| L689I (Q) | 5 | 0.02 ± 0.01 (0.52) | 0.01 ± 0.00 (0.24) | 0.01 ± 0.01 (0.24) | 0.96 ± 0.01 | 4 |
| L689I (Q) | 40 | 0.03 ± 0.01 (0.34) | 0.03 ± 0.03 (0.35) | 0.03 ± 0.02 (0.32) | 0.92 ± 0.04 | 4 |
| L689I (Q) | 160 | 0.11 ± 0.08 (0.43) | 0.09 ± 0.06 (0.37) | 0.05 ± 0.02 (0.20) | 0.76 ± 0.08 | 4 |
| L689I DI (F) | 5 | 0.15 ± 0.03 (0.84) | 0.01 ± 0.01 (0.05) | 0.02 ± 0.02 (0.11) | 0.81 ± 0.02 | 3 |
| L689I DI (F) | 40 | 0.18 ± 0.01 (0.80) | 0.00 ± 0.00 (0.00)a | 0.05 ± 0.03 (0.20)a | 0.78 ± 0.03 | 3 |
| L689I DI (F) | 160 | 0.11 ± 0.29 (0.35) | 0.08 ± 0.03 (0.27) | 0.12 ± 0.06 (0.38) | 0.69 ± 0.05 | 3 |
| L689I DII (F) | 5 | 0.09 ± 0.01 (0.89) | 0.01 ± 0.01 (0.11) | 0.00 ± 0.00 (0.00) | 0.90 ± 0.02 | 3 |
| L689I DII (F) | 40 | 0.15 ± 0.07 (0.63) | 0.04 ± 0.02 (0.19) | 0.04 ± 0.02 (0.18) | 0.77 ± 0.03 | 3 |
| L689I DII (F) | 160 | 0.21 ± 0.09 (0.70) | 0.00 ± 0.00 (0.00) | 0.09 ± 0.05 (0.31) | 0.69 ± 0.05 | 3 |
| L689I DIII (F) | 5 | 0.38 ± 0.10 (0.82) | 0.02 ± 0.02 (0.04) | 0.06 ± 0.04 (0.14) | 0.54 ± 0.09 | 4 |
| L689I DIII (F) | 40 | 0.30 ± 0.14 (0.49) | 0.13 ± 0.10 (0.21) | 0.19 ± 0.05 (0.30) | 0.38 ± 0.15 | 4 |
| L689I DIII (F) | 160 | 0.04 ± 0.04 (0.06) | 0.31 ± 0.11 (0.42) | 0.39 ± 0.10 (0.53) | 0.26 ± 0.06 | 4 |
| L689I DIV (F) | 5 | 0.12 ± 0.06 (0.57) | 0.01 ± 0.01 (0.04) | 0.08 ± 0.05 (0.39) | 0.79 ± 0.06 | 5 |
| L689I DIV (F) | 40 | 0.25 ± 0.07 (0.75)a | 0.07 ± 0.04 (0.22)a | 0.01 ± 0.01 (0.03) | 0.67 ± 0.12 | 5 |
| L689I DIV (F) | 160 | 0.13 ± 0.05 (0.38) | 0.04 ± 0.02 (0.12) | 0.16 ± 0.08 (0.39) | 0.68 ± 0.07 | 5 |
Similar to SI onset, recovery from SI can be fit with three time constants: τF = 0.6 ± 0.3 s, τI = 4.5 ± 1.1 s, and τS = 43.1 ± 4.8 s, which were found by fitting the recovery of the WT ionic current after a 160-s depolarizing pulse to +45. The fractional magnitudes below are for pulses to +45 mV lasting 5, 40, and 160 s. I, the ionic current recovery in Fig. 3 A; Q, the gating current recovery in Fig. 3 B; DI, DII, DIII, and DIV, the fluorescence recoveries in Fig. 3 C. Similarly, the L689I magnitudes are for experimental data shown in Fig. 4. The fraction of inactivation corresponding to a given time constant is in parentheses. Channel variant abbreviations as in Table 1.
Significantly different from the WT parameter (P < 0.05).