General characteristics of wild-type larvae, and larvae injected with control, splice-blocking, and translation-blocking MO
| Criteria | Wild type | Control | Splicing MO | Translation MO |
| Survival rate (%) | >95 | 74 ± 7 | 47 ± 7a | 48 ± 5a |
| Hatching rate (%) | >85 | 86 ± 3 | 47 ± 7b | 42 ± 7b |
| Body length (mm) | 3.6 ± 0.13 | 3.6 ± 0.04 | 3.2 ± 0.06b | 3.04 ± 0.11b |
| lardur (s) | 60 | 22 ± 9d | ||
| lardist (cm) | 141 ± 13 | 36 ± 19d | ||
| Speed (cm/s) | 2.4 ± 0.2 | 1.2 ± 0.5c |
| Criteria | Wild type | Control | Splicing MO | Translation MO |
| Survival rate (%) | >95 | 74 ± 7 | 47 ± 7a | 48 ± 5a |
| Hatching rate (%) | >85 | 86 ± 3 | 47 ± 7b | 42 ± 7b |
| Body length (mm) | 3.6 ± 0.13 | 3.6 ± 0.04 | 3.2 ± 0.06b | 3.04 ± 0.11b |
| lardur (s) | 60 | 22 ± 9d | ||
| lardist (cm) | 141 ± 13 | 36 ± 19d | ||
| Speed (cm/s) | 2.4 ± 0.2 | 1.2 ± 0.5c |
The mean values in the table are calculated as mean rates of 24 hpf (hour postfertilization) survival and 3 dpf hatching (among the surviving eggs), determined in three independent injection rounds (each comprising 50–100 eggs). Body length was measured at 4 dpf, from the snout to the posterior end of the larval body. The measurements of swimming behavior of 4-dpf controls (n = 10) and desmin-splicing morphants (n = 7) were recorded during 60 s. The lardur parameter is the mean time spent by larvae in large movements, lardist is the distance covered by larvae during large movements, and speed is calculated by lardist/lardur.
Significant difference (P < 0.05) compared with the control group (ANOVA, using the Holm-Sidak method).
Significant difference (P < 0.01) compared with the control group (ANOVA, using the Holm-Sidak method).
Significant difference (P < 0.05) compared with the control group (Student’s t test).
Significant difference (P < 0.001) compared with the control group (Student’s t test).