Table 1.

Biophysical properties of NaV1.9 in the absence (control) or presence of intracellular GTPγS

Condition Peak current density (−40 mV) Voltage dependence of activation Steady-state inactivation Recovery from inactivation 
 pA/pF n V1/2 k n V1/2 k n τ1 τ2 n 
   mV   mV   ms ms  
Control −23.2 ± 3.4 16 −56.9 ± 0.6 6.7 ± 0.3 16 −52.1 ± 2.6 9.2 ± 0.7 7 13.6 ± 2.2 77.9 ± 11.5 8 
         (52.3 ± 7.3%) (24.4 ± 6.9%)  
GTPγS −38.9 ± 5.1a 20 −54.3 ± 1.1 5.8 ± 0.3b 20 −43.6 ± 2.0c 10.4 ± 0.7 8 16.0 ± 1.0 466.9 ± 151.9d 6 
         (61.7 ± 3.9%) (18.0 ± 3.2%)  
Condition Peak current density (−40 mV) Voltage dependence of activation Steady-state inactivation Recovery from inactivation 
 pA/pF n V1/2 k n V1/2 k n τ1 τ2 n 
   mV   mV   ms ms  
Control −23.2 ± 3.4 16 −56.9 ± 0.6 6.7 ± 0.3 16 −52.1 ± 2.6 9.2 ± 0.7 7 13.6 ± 2.2 77.9 ± 11.5 8 
         (52.3 ± 7.3%) (24.4 ± 6.9%)  
GTPγS −38.9 ± 5.1a 20 −54.3 ± 1.1 5.8 ± 0.3b 20 −43.6 ± 2.0c 10.4 ± 0.7 8 16.0 ± 1.0 466.9 ± 151.9d 6 
         (61.7 ± 3.9%) (18.0 ± 3.2%)  

Steady-state inactivation was determined using a 300-ms prepulse to various potentials followed by a 50-ms pulse to −40 mV. Recovery from inactivation was determined after a 300-ms prepulse to −40 mV.

a

P = 0.025 (GTPγS vs. control using Student’s t test).

b

P = 0.015 (GTPγS vs. control using Student’s t test).

c

P = 0.021 (GTPγS vs. control using Student’s t test).

d

P = 0.011 (GTPγS vs. control using Student’s t test).

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