Estimations of the distances between cytoplasmic regulatory Ca2+-binding sites and the channel pore axis
| Experimental conditions | ||||||||||||
| Representative current trace | [InsP3] | [Ca2+]ERa | Bulk [Ca2+]ib | Vapp | Cytoplasmic Ca2+ buffering | Mean Po | iCa | iCa Po | [Ca2+]i profiles | Regulatory Ca2+ site | [Ca2+]i at site | Range of distance from pore |
| µM | mV | fA | fA | µM | nm | |||||||
| Fig. 2 (A and B) | 3 | 300 µM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.22 | 230 | 51 | Fig. 11 A | Inhibitory | 5.7 µM | 12 < x < 39 |
| Fig. 2 C | 10 | 300 µM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.7 | 230 | 161 | Fig. 11 B | Inhibitory | 7.2 µM | 23 < x < 31 |
| Fig. 3 A | 10 | 1.1 mM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.46 | 830 | 380 | Fig. 11 C | Inhibitory | 14.3 µM | 25 < x < 42 |
| Fig. 6 A | 3 | 300 µM | 2 µM | −30 | 0.1 mM HEDTA | 0.09 | 230 | 21 | Fig. 11 D | Inhibitory | 12 µM | 2 < x < 21 |
| Fig. 6 B | 3 | 300 µM | 2 µM | −30 | 5 mM diBrBAPTA | 0.4 | 230 | 92 | Fig. 11 E | Inhibitory | 3.1 µM | 29 < x < 44 |
| Fig. 8 A | 10 | 1.1 mM | 2 µM | +30 | 5 mM diBrBAPTA | 0.7 | 75 | 53 | Fig. 11 F | Inhibitory | 3.1 µM | 22 < x < 27 |
| Fig. 4 A | 10 | 300 µM | 55 nM | −30 | 0.5 mM BAPTA | 0.29 | 230 | 64 | Fig. 11 G | Activating | 500 nM | x < 62 |
| Fig. 5 A | 10 | 300 µM | 55 nM | +30 | 0.5 mM BAPTA | 0.12 | 20 | 2.5 | Fig. 11 H | Activating | 150 nM | x < 22 |
| Fig. 9 A | 10 | 550 µM | 55 nM | +30 | 10 mM BAPTA | 0.25 | 38 | 10 | Fig. 11 I | Activating | 410 nM | x < 13 |
| Fig. 9 B | 10 | 550 µM | 55 nM | +50 | 10 mM BAPTA | 0.08 | 12 | 1 | Fig. 11 J | Activating | 100 nM | x < 14 |
| Fig. 9 C | 10 | 550 µM | 55 nM | +70 | 10 mM BAPTA | 0.05 | 3 | 0.15 | Fig. 11 K | Activating | 80 nM | x < 9 |
| Experimental conditions | ||||||||||||
| Representative current trace | [InsP3] | [Ca2+]ERa | Bulk [Ca2+]ib | Vapp | Cytoplasmic Ca2+ buffering | Mean Po | iCa | iCa Po | [Ca2+]i profiles | Regulatory Ca2+ site | [Ca2+]i at site | Range of distance from pore |
| µM | mV | fA | fA | µM | nm | |||||||
| Fig. 2 (A and B) | 3 | 300 µM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.22 | 230 | 51 | Fig. 11 A | Inhibitory | 5.7 µM | 12 < x < 39 |
| Fig. 2 C | 10 | 300 µM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.7 | 230 | 161 | Fig. 11 B | Inhibitory | 7.2 µM | 23 < x < 31 |
| Fig. 3 A | 10 | 1.1 mM | 2 µM | −30 | 0.5 mM diBrBAPTA | 0.46 | 830 | 380 | Fig. 11 C | Inhibitory | 14.3 µM | 25 < x < 42 |
| Fig. 6 A | 3 | 300 µM | 2 µM | −30 | 0.1 mM HEDTA | 0.09 | 230 | 21 | Fig. 11 D | Inhibitory | 12 µM | 2 < x < 21 |
| Fig. 6 B | 3 | 300 µM | 2 µM | −30 | 5 mM diBrBAPTA | 0.4 | 230 | 92 | Fig. 11 E | Inhibitory | 3.1 µM | 29 < x < 44 |
| Fig. 8 A | 10 | 1.1 mM | 2 µM | +30 | 5 mM diBrBAPTA | 0.7 | 75 | 53 | Fig. 11 F | Inhibitory | 3.1 µM | 22 < x < 27 |
| Fig. 4 A | 10 | 300 µM | 55 nM | −30 | 0.5 mM BAPTA | 0.29 | 230 | 64 | Fig. 11 G | Activating | 500 nM | x < 62 |
| Fig. 5 A | 10 | 300 µM | 55 nM | +30 | 0.5 mM BAPTA | 0.12 | 20 | 2.5 | Fig. 11 H | Activating | 150 nM | x < 22 |
| Fig. 9 A | 10 | 550 µM | 55 nM | +30 | 10 mM BAPTA | 0.25 | 38 | 10 | Fig. 11 I | Activating | 410 nM | x < 13 |
| Fig. 9 B | 10 | 550 µM | 55 nM | +50 | 10 mM BAPTA | 0.08 | 12 | 1 | Fig. 11 J | Activating | 100 nM | x < 14 |
| Fig. 9 C | 10 | 550 µM | 55 nM | +70 | 10 mM BAPTA | 0.05 | 3 | 0.15 | Fig. 11 K | Activating | 80 nM | x < 9 |