Effect of electrical stimulation on the uptake of 36Cl in isolated rat EDL muscle in vitro
| Experimental conditions | 36Cl uptake | Intracellular 36Cl | Relative increase | P-values |
| µmol/g wet wt | mM | % | ||
| Resting controls | 7.1 ± 0.6 (5) | 13.9 ± 1.2 | ||
| 60-Hz stimulation, 60 s | 12.2 ± 1.8 (5) | 23.9 ± 3.6 | 71 | <0.03 |
| Resting controls | 7.6 ± 0.3 (9) | 14.9 ± 0.6 | ||
| 5-Hz stimulation, 300 s | 16.4 ± 0.7 (3) | 32.2 ± 1.4 | 116 | <0.001 |
| 10-Hz stimulation, 120 s | 15.2 ± 1.0 (3) | 29.9 ± 2.0 | 100 | <0.001 |
| 10-Hz stimulation, 300 s | 19.5 ± 0.4 (3) | 38.2 ± 7.8 | 157 | <0.001 |
| 20-Hz stimulation, 120 s | 21.9 ± 4.2 (3) | 42.9 ± 8.4 | 188 | <0.001 |
| Experimental conditions | 36Cl uptake | Intracellular 36Cl | Relative increase | P-values |
| µmol/g wet wt | mM | % | ||
| Resting controls | 7.1 ± 0.6 (5) | 13.9 ± 1.2 | ||
| 60-Hz stimulation, 60 s | 12.2 ± 1.8 (5) | 23.9 ± 3.6 | 71 | <0.03 |
| Resting controls | 7.6 ± 0.3 (9) | 14.9 ± 0.6 | ||
| 5-Hz stimulation, 300 s | 16.4 ± 0.7 (3) | 32.2 ± 1.4 | 116 | <0.001 |
| 10-Hz stimulation, 120 s | 15.2 ± 1.0 (3) | 29.9 ± 2.0 | 100 | <0.001 |
| 10-Hz stimulation, 300 s | 19.5 ± 0.4 (3) | 38.2 ± 7.8 | 157 | <0.001 |
| 20-Hz stimulation, 120 s | 21.9 ± 4.2 (3) | 42.9 ± 8.4 | 188 | <0.001 |
Experimental conditions as described in the legend to Fig. 3, except that stimulation was also performed using the indicated lower frequencies and longer durations before the quadruple 15-min washout in Na+-free Tris-sucrose buffer at 0°C. All values were corrected for the loss of 36Cl taking place during the washout in the cold and are given as µmol/g wet wt ± SEM, with the number of muscles in parentheses.