Fitted parameters for WT and mutant channels
| z1 | z2 | V1 (mV) | V2 (mV) | r2 | |
| WT | 2.37 | 4.19 | −49.6 | −33.9 | n.d. |
| R4K | 2.03 ± 0.11 | 0.93 ± 0.06 | −60.7 ± 0.9 | 46.4 ± 3.2 | 0.9973 |
| I241W | 2.07 ± 0.04 | 1.91 ± 0.04 | −74.4 ± 0.4 | 9.8 ± 0.4 | 0.9995 |
| I241W/R1K | 1.53 ± 0.04 | 2.01 ± 0.05 | −68.0 ± 0.7 | 49.6 ± 0.5 | 0.9995 |
| I241W/R2K | 1.62 ± 0.02 | 1.48 ± 0.02 | −59.2 ± 0.3 | 4.4 ± 0.3 | 0.9999 |
| I241W/R2Q | 1.23 ± 0.07 | 2.11 ± 0.11 | −89.9 ± 2.2 | 18.8 ± 0.9 | 0.9974 |
| I241W/R3K | 1.84 ± 0.04 | 2.12 ± 0.04 | −93.0 ± 0.5 | 57.7 ± 0.4 | 0.9997 |
| I241W/R4K | 1.91 ± 0.08 | 1.75 ± 0.07 | −79.1 ± 0.8 | 88.9 ± 0.1 | 0.9978 |
| F244W | 2.65 ± 0.10 | 2.29 ± 0.09 | −63.4 ± 0.5 | −2.3 ± 0.7 | 0.9993 |
| F244W/R2K | 2.61 ± 0.04 | 2.17 ± 0.03 | −53.7 ± 0.2 | −12.7 ± 0.2 | 0.9999 |
| F244W/R3K | 2.01 ± 0.09 | 2.39 ± 0.11 | −87.6 ± 1.1 | 8.6 ± 0.8 | 0.9978 |
| Deac I241W | 2.62 ± 0.19 | 1.89 ± 0.15 | −71.7 ± 0.1 | −9.9 ± 1.7 | 0.9976 |
| Deac F244W | 3.56 ± 0.15 | 2.99 ± 0.15 | −64.5 ± 0.4 | −15.0 ± 0.6 | 0.9986 |
| Deac I241W/R2K | 2.52 ± 0.12 | 2.31 ± 0.11 | −37.7 ± 0.7 | 6.2 ± 0.7 | 0.9994 |
| Deac I241W/R3K | 2.25 ± 0.15 | 2.02 ± 0.14 | −91.5 ± 1.2 | −20.7 ± 1.4 | 0.9972 |
| z1 | z2 | V1 (mV) | V2 (mV) | r2 | |
| WT | 2.37 | 4.19 | −49.6 | −33.9 | n.d. |
| R4K | 2.03 ± 0.11 | 0.93 ± 0.06 | −60.7 ± 0.9 | 46.4 ± 3.2 | 0.9973 |
| I241W | 2.07 ± 0.04 | 1.91 ± 0.04 | −74.4 ± 0.4 | 9.8 ± 0.4 | 0.9995 |
| I241W/R1K | 1.53 ± 0.04 | 2.01 ± 0.05 | −68.0 ± 0.7 | 49.6 ± 0.5 | 0.9995 |
| I241W/R2K | 1.62 ± 0.02 | 1.48 ± 0.02 | −59.2 ± 0.3 | 4.4 ± 0.3 | 0.9999 |
| I241W/R2Q | 1.23 ± 0.07 | 2.11 ± 0.11 | −89.9 ± 2.2 | 18.8 ± 0.9 | 0.9974 |
| I241W/R3K | 1.84 ± 0.04 | 2.12 ± 0.04 | −93.0 ± 0.5 | 57.7 ± 0.4 | 0.9997 |
| I241W/R4K | 1.91 ± 0.08 | 1.75 ± 0.07 | −79.1 ± 0.8 | 88.9 ± 0.1 | 0.9978 |
| F244W | 2.65 ± 0.10 | 2.29 ± 0.09 | −63.4 ± 0.5 | −2.3 ± 0.7 | 0.9993 |
| F244W/R2K | 2.61 ± 0.04 | 2.17 ± 0.03 | −53.7 ± 0.2 | −12.7 ± 0.2 | 0.9999 |
| F244W/R3K | 2.01 ± 0.09 | 2.39 ± 0.11 | −87.6 ± 1.1 | 8.6 ± 0.8 | 0.9978 |
| Deac I241W | 2.62 ± 0.19 | 1.89 ± 0.15 | −71.7 ± 0.1 | −9.9 ± 1.7 | 0.9976 |
| Deac F244W | 3.56 ± 0.15 | 2.99 ± 0.15 | −64.5 ± 0.4 | −15.0 ± 0.6 | 0.9986 |
| Deac I241W/R2K | 2.52 ± 0.12 | 2.31 ± 0.11 | −37.7 ± 0.7 | 6.2 ± 0.7 | 0.9994 |
| Deac I241W/R3K | 2.25 ± 0.15 | 2.02 ± 0.14 | −91.5 ± 1.2 | −20.7 ± 1.4 | 0.9972 |
The Q-V curves of the single or double mutants were fitted using a 3-state model equation (see Appendix). The parameters for WT were from a previous work (Bezanilla and Stefani, 1996). The fitted parameters for deactivation (Deac) were obtained from experiments done at 28°C.