Table 1. Biochemical properties of... | Rockefeller University Press

Table 1.

Biochemical properties of 11-cis-retinal and 11-cis 4-OH retinal

Biochemical property	11-cis-retinal	11-cis 4-OH retinal
Fractional solubility^a
Ringer’s solution	0.34	1
Ringer’s solution, 0.01% BSA	0.74	1
Partition coefficient^b
K_eq	(1.6 ± 0.5) × 10⁸	(2.9 ± 0.2) × 10⁶
Pigment formation rate constant, R^c
Bovine 2/282 opsin, micelles	0.74 s⁻¹	0.15 s⁻¹
Bovine 2/282 opsin, nanodiscs	0.019 s⁻¹	0.010 s⁻¹
Bovine WT opsin, nanodiscs	0.015 s⁻¹	0.007 s⁻¹
Salamander opsin, nanodiscs	0.053 s⁻¹	0.027 s⁻¹

Biochemical property	11-cis-retinal	11-cis 4-OH retinal
Fractional solubility^a
Ringer’s solution	0.34	1
Ringer’s solution, 0.01% BSA	0.74	1
Partition coefficient^b
K_eq	(1.6 ± 0.5) × 10⁸	(2.9 ± 0.2) × 10⁶
Pigment formation rate constant, R^c
Bovine 2/282 opsin, micelles	0.74 s⁻¹	0.15 s⁻¹
Bovine 2/282 opsin, nanodiscs	0.019 s⁻¹	0.010 s⁻¹
Bovine WT opsin, nanodiscs	0.015 s⁻¹	0.007 s⁻¹
Salamander opsin, nanodiscs	0.053 s⁻¹	0.027 s⁻¹

Calculated for 10 µM retinal. We assume the same extinction coefficient for 11-cis-retinal and 11-cis 4-OH retinal.

Calculated according to Eq. 1 (see Materials and methods for details).

From fits of single exponential functions to the data presented in Fig. 5.