Shifts in Vmedian and in V1/2 occurring with Cd2+
| Parameter | Vmedian | V1/2 |
| 462C466C QON (0 mM Cd2+) | −117.9 ± 4.5 | −132.7 ± 5.7 |
| QON (130 mM Cd2+) | −89.9 ± 10.3 | −103.9 ± 8.9 |
| QOFF (0 mM Cd2+) | −112.9 ± 3.2 | −126.7 ± 4.2 |
| QOFF (130 mM Cd2+) | −101.5 ± 4.4 | −99.9 ± 5.4 |
| 462Y468C QON (0 mM Cd2+) | −91.9 ± 8.4 | −116.4 ± 4.8 |
| QON (130 mM Cd2+) | −104.8 ± 7.9 | −131.8 ± 3.9 |
| QOFF (0 mM Cd2+) | −94.7 ± 6.5 | −96.7 ± 4.6 |
| QOFF (130 mM Cd2+) | −106.6 ± 6.4 | −128.0 ± 4.2 |
| Parameter | Vmedian | V1/2 |
| 462C466C QON (0 mM Cd2+) | −117.9 ± 4.5 | −132.7 ± 5.7 |
| QON (130 mM Cd2+) | −89.9 ± 10.3 | −103.9 ± 8.9 |
| QOFF (0 mM Cd2+) | −112.9 ± 3.2 | −126.7 ± 4.2 |
| QOFF (130 mM Cd2+) | −101.5 ± 4.4 | −99.9 ± 5.4 |
| 462Y468C QON (0 mM Cd2+) | −91.9 ± 8.4 | −116.4 ± 4.8 |
| QON (130 mM Cd2+) | −104.8 ± 7.9 | −131.8 ± 3.9 |
| QOFF (0 mM Cd2+) | −94.7 ± 6.5 | −96.7 ± 4.6 |
| QOFF (130 mM Cd2+) | −106.6 ± 6.4 | −128.0 ± 4.2 |
The trapezoid method (Chowdhury and Chanda, 2012) was used to calculate the Vmedian values from locked-open and locked-closed mutants, and V1/2 values were calculated from a single Boltzmann fit.