Table 1. Kinetic attributes of NMDA... | Rockefeller University Press

Table 1.

Kinetic attributes of NMDA receptors with A7 and A8 substitutions

Mutation		Amplitude	P_o	Mean open time	Mean closed time	n	Duration	Events ×10⁶
		pA		ms	ms		min
WT	N1/N2	10.0 ± 0.6	0.54 ± 0.03	9 ± 1	8 ± 1	5	244	1.5
A8T	N1^8T/N2	7.3 ± 0.3^a	0.48 ± 0.06	17 ± 4	21 ± 5	12	552	2.8
A8T	N1/N2^8T	7.5 ± 0.5^a	0.56 ± 0.05	11 ± 1	10 ± 2	7	266	1.7
A8Y	N1^8Y/N2	8.3 ± 0.8	0.56 ± 0.1	9 ± 2	8 ± 3	6	441	3.1
A8Y	N1/N2^8Y	7.1 ± 0.3^a	0.11 ± 0.03^a	5 ± 0.4^a	56 ± 14^a	7	198	0.46
A7Y	N1^7Y/N2	7.2 ± 0.2^a	0.7 ± 0.03^a	65 ± 13^a	28 ± 6	5	184	0.23
	(−/Gly)	5.9 ± 0.4^a^b	0.24 ± 0.04^a^b	30 ± 2^a^b	112 ± 25^a^b	5	184	0.19
	(−/−)	5.8 ± 0.2^a^b	0.06 ± 0.01^a^b	77 ± 8^a	1,400 ± 400^a^b	5	370	0.03
	N1/N2^7Y	6.2 ± 0.2^a	0.81 ± 0.06^a	110 ± 10^a	29 ± 13	5	190	0.18

Mutation		Amplitude	P_o	Mean open time	Mean closed time	n	Duration	Events ×10⁶
		pA		ms	ms		min
WT	N1/N2	10.0 ± 0.6	0.54 ± 0.03	9 ± 1	8 ± 1	5	244	1.5
A8T	N1^8T/N2	7.3 ± 0.3^a	0.48 ± 0.06	17 ± 4	21 ± 5	12	552	2.8
A8T	N1/N2^8T	7.5 ± 0.5^a	0.56 ± 0.05	11 ± 1	10 ± 2	7	266	1.7
A8Y	N1^8Y/N2	8.3 ± 0.8	0.56 ± 0.1	9 ± 2	8 ± 3	6	441	3.1
A8Y	N1/N2^8Y	7.1 ± 0.3^a	0.11 ± 0.03^a	5 ± 0.4^a	56 ± 14^a	7	198	0.46
A7Y	N1^7Y/N2	7.2 ± 0.2^a	0.7 ± 0.03^a	65 ± 13^a	28 ± 6	5	184	0.23
	(−/Gly)	5.9 ± 0.4^a^b	0.24 ± 0.04^a^b	30 ± 2^a^b	112 ± 25^a^b	5	184	0.19
	(−/−)	5.8 ± 0.2^a^b	0.06 ± 0.01^a^b	77 ± 8^a	1,400 ± 400^a^b	5	370	0.03
	N1/N2^7Y	6.2 ± 0.2^a	0.81 ± 0.06^a	110 ± 10^a	29 ± 13	5	190	0.18

Significant difference relative to WT; P < 0.05 (Student’s t test).

Significant difference relative to N1^7Y/N2 with Glu/Gly; P < 0.05 (Student’s t test).