Table 2.

Known acetylation sites on UniProt among ETC and TCA proteins in the mouse and human heart

Protein name Species UniProt accession Acetylation site(s) 
Aconitase Human Q99798 K50, K573, K605 
 Mouse Q99KI0 K50 
Acyl carrier protein Human O14561 K92 
ATP synthase protein 8 Mouse P03930 K54 
ATP synthase subunit b Human P24539 K221, K233 
 Mouse Q9CQQ7 K115, K131, K162, K188, K221, K225, K233 
ATP synthase subunit d Human O75947 K85, K95, K117, K149 
 Mouse Q9DCX2 K63, K78, K85, K99, K117, K149 
ATP synthase subunit f Human P56134 A2 
ATP synthase subunit g Human O75964 A2, K24 
 Mouse Q9CPQ8 K54, K66 
ATP synthase subunit γ Human P36542 K55, K154, K197 
 Mouse Q91VR2 K79, K90, K115 
ATP synthase subunit O Human P48047 K54, K60, K70, K162, K172, K192 
 Mouse Q9DB20 K60, K70, K158, K162, K172, K176, K192 
ATP synthase subunit α Human P25705 K161, K261, K305, K434, K498, K506, K539 
 Mouse Q03265 K132, K230, K239, K261, K305, K427, K498, K531, K539 
ATP synthase subunit β Human P06576 K133, K198, K426, K485 
 Mouse P56480 K133, K259, K522 
ATP synthase-coupling factor 6 Human P18859 K41, K46, K99, K105 
 Mouse P97450 K84, K99 
Citrate synthase Human O75390 K327, K366, K375, K382, K393 
Cytochrome b-c1 complex subunit 1 Human P31930 K111 
Cytochrome b-c1 complex subunit 2 Mouse Q9DB77 K159, K250 
Cytochrome b-c1 complex subunit 6 Human P07919 K85 
 Mouse P99028 K40 
Cytochrome b-c1 complex subunit 7 Human P14927 K78 
Cytochrome c oxidase polypeptide VIc Mouse Q9CPQ1 K61 
Cytochrome c oxidase subunit 4 isoform 1 Human P13073 K53, K60 
Cytochrome c oxidase subunit 5B Human P10606 K121 
Cytochrome c oxidase subunit 7A-related Human O14548 K69 
Dihydrolipoyl dehydrogenase Human P09622 K143, K267, K320, K410, K417, K420 
 Mouse O08749 K127 
Fumarate hydratase Human P07954 K66, K80, K94, K256, K292 
 Mouse P97807 K63, K112, K474 
Isocitrate dehydrogenase subunit β Human O43837 K146, K199, K374 
Isocitric dehydrogenase subunit α Human P50213 K343 
Malate dehydrogenase Human P40926 K165, K185, K301, K307, K314, K329, K335 
 Mouse P08249 K157, K239, K314 
NADH-ubiquinone oxidoreductase 13-kD A subunit Mouse P52503 K112 
NADH-ubiquinone oxidoreductase 13-kD B subunit Mouse Q9CPP6 K36, K46, K98 
NADH-ubiquinone oxidoreductase 39-kD subunit Mouse Q9DC69 K370 
NADH-ubiquinone oxidoreductase 42-kD subunit Mouse Q99LC3 K122, K181, K242, K350 
NADH-ubiquinone oxidoreductase 51-kD subunit Mouse Q91YT0 K81, K104, K375 
NADH-ubiquinone oxidoreductase 75-kD subunit Mouse Q91VD9 K84 
NADH-ubiquinone oxidoreductase B12 subunit Mouse Q9CQZ6 K29, K40 
NADH-ubiquinone oxidoreductase B22 subunit Human Q9Y6M9 A2 
NADH-ubiquinone oxidoreductase B8 subunit Human O43678 A2 
 Mouse Q9CQ75 K64, K75 
NADH-ubiquinone oxidoreductase MLRQ subunit Mouse Q62425 K56 
NADH-ubiquinone oxidoreductase subunit B14.5b Human O95298 K114 
NADH-ubiquinone oxidoreductase subunit B17.2 Mouse Q7TMF3 K43, K47 
Succinate dehydrogenase flavoprotein subunit Human P31040 K179, K335, K541, K547, K608 
 Mouse Q8K2B3 K179, K423, K485, K498, K538, K547 
Succinyl-CoA synthetase β A chain Human Q9P2R7 K78, K143 
Succinyl-CoA synthetase β G chain Human Q96I99 K73, K227, K291, K338 
Succinyl-CoA synthetase subunit α Human P53597 K54 
Protein name Species UniProt accession Acetylation site(s) 
Aconitase Human Q99798 K50, K573, K605 
 Mouse Q99KI0 K50 
Acyl carrier protein Human O14561 K92 
ATP synthase protein 8 Mouse P03930 K54 
ATP synthase subunit b Human P24539 K221, K233 
 Mouse Q9CQQ7 K115, K131, K162, K188, K221, K225, K233 
ATP synthase subunit d Human O75947 K85, K95, K117, K149 
 Mouse Q9DCX2 K63, K78, K85, K99, K117, K149 
ATP synthase subunit f Human P56134 A2 
ATP synthase subunit g Human O75964 A2, K24 
 Mouse Q9CPQ8 K54, K66 
ATP synthase subunit γ Human P36542 K55, K154, K197 
 Mouse Q91VR2 K79, K90, K115 
ATP synthase subunit O Human P48047 K54, K60, K70, K162, K172, K192 
 Mouse Q9DB20 K60, K70, K158, K162, K172, K176, K192 
ATP synthase subunit α Human P25705 K161, K261, K305, K434, K498, K506, K539 
 Mouse Q03265 K132, K230, K239, K261, K305, K427, K498, K531, K539 
ATP synthase subunit β Human P06576 K133, K198, K426, K485 
 Mouse P56480 K133, K259, K522 
ATP synthase-coupling factor 6 Human P18859 K41, K46, K99, K105 
 Mouse P97450 K84, K99 
Citrate synthase Human O75390 K327, K366, K375, K382, K393 
Cytochrome b-c1 complex subunit 1 Human P31930 K111 
Cytochrome b-c1 complex subunit 2 Mouse Q9DB77 K159, K250 
Cytochrome b-c1 complex subunit 6 Human P07919 K85 
 Mouse P99028 K40 
Cytochrome b-c1 complex subunit 7 Human P14927 K78 
Cytochrome c oxidase polypeptide VIc Mouse Q9CPQ1 K61 
Cytochrome c oxidase subunit 4 isoform 1 Human P13073 K53, K60 
Cytochrome c oxidase subunit 5B Human P10606 K121 
Cytochrome c oxidase subunit 7A-related Human O14548 K69 
Dihydrolipoyl dehydrogenase Human P09622 K143, K267, K320, K410, K417, K420 
 Mouse O08749 K127 
Fumarate hydratase Human P07954 K66, K80, K94, K256, K292 
 Mouse P97807 K63, K112, K474 
Isocitrate dehydrogenase subunit β Human O43837 K146, K199, K374 
Isocitric dehydrogenase subunit α Human P50213 K343 
Malate dehydrogenase Human P40926 K165, K185, K301, K307, K314, K329, K335 
 Mouse P08249 K157, K239, K314 
NADH-ubiquinone oxidoreductase 13-kD A subunit Mouse P52503 K112 
NADH-ubiquinone oxidoreductase 13-kD B subunit Mouse Q9CPP6 K36, K46, K98 
NADH-ubiquinone oxidoreductase 39-kD subunit Mouse Q9DC69 K370 
NADH-ubiquinone oxidoreductase 42-kD subunit Mouse Q99LC3 K122, K181, K242, K350 
NADH-ubiquinone oxidoreductase 51-kD subunit Mouse Q91YT0 K81, K104, K375 
NADH-ubiquinone oxidoreductase 75-kD subunit Mouse Q91VD9 K84 
NADH-ubiquinone oxidoreductase B12 subunit Mouse Q9CQZ6 K29, K40 
NADH-ubiquinone oxidoreductase B22 subunit Human Q9Y6M9 A2 
NADH-ubiquinone oxidoreductase B8 subunit Human O43678 A2 
 Mouse Q9CQ75 K64, K75 
NADH-ubiquinone oxidoreductase MLRQ subunit Mouse Q62425 K56 
NADH-ubiquinone oxidoreductase subunit B14.5b Human O95298 K114 
NADH-ubiquinone oxidoreductase subunit B17.2 Mouse Q7TMF3 K43, K47 
Succinate dehydrogenase flavoprotein subunit Human P31040 K179, K335, K541, K547, K608 
 Mouse Q8K2B3 K179, K423, K485, K498, K538, K547 
Succinyl-CoA synthetase β A chain Human Q9P2R7 K78, K143 
Succinyl-CoA synthetase β G chain Human Q96I99 K73, K227, K291, K338 
Succinyl-CoA synthetase subunit α Human P53597 K54 

Proteins from ETC complexes and the TCA cycle were experimentally determined from mouse and human heart mitochondria. The PTM information of the protein was retrieved from UniProt. The referenced acetylation sites were identified from their respective species but may have originated from different tissues other than the heart (e.g. mouse liver, human HeLa cells). Tissue-specific PTM distribution is a matter of intense investigation.

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