Free concentrations of Zn2+ and Cu2+ in 10 mM Tricine
Zn2+
Added, µM
9.7
100
900
Free, µM
0.08
0.8
8
Cu2+
Added, µM
30
100
269
616
1,100
Free, nM
13
45
123
293
551
Zn2+
Added, µM
9.7
100
900
Free, µM
0.08
0.8
8
Cu2+
Added, µM
30
100
269
616
1,100
Free, nM
13
45
123
293
551
Free concentrations were calculated using the Solver function in the MaxChelator program. Calculations took into account the presence of 1 mM Ca2+, ionic strength (0.15 M), and pH (7.4).