Free concentrations of Zn2+ and Cu2+ in 10 mM Tricine
| Zn2+ | Added, µM | 9.7 | 100 | 900 | ||
| Free, µM | 0.08 | 0.8 | 8 | |||
| Cu2+ | Added, µM | 30 | 100 | 269 | 616 | 1,100 |
| Free, nM | 13 | 45 | 123 | 293 | 551 |
| Zn2+ | Added, µM | 9.7 | 100 | 900 | ||
| Free, µM | 0.08 | 0.8 | 8 | |||
| Cu2+ | Added, µM | 30 | 100 | 269 | 616 | 1,100 |
| Free, nM | 13 | 45 | 123 | 293 | 551 |
Free concentrations were calculated using the Solver function in the MaxChelator program. Calculations took into account the presence of 1 mM Ca2+, ionic strength (0.15 M), and pH (7.4).
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