Table 1. Biophysical properties Solution... | Rockefeller University Press

Table 1.

Biophysical properties

Solution	Activation				Inactivation			Kinetics
Ca_v2.3 WT	P_Ca(max) ± SEM	V_1/2 ± SEM	k ± SEM	n	V_1/2 ± SEM	k ± SEM	n	A_f ± SEM	τ_f ± SEM	A_s ± SEM	τ_s ± SEM	n
HEPES	0.011 ± 0.003	1.5 ± 1.0^a	7.2 ± 0.1^a	8	−36.1 ± 1.1^b	8.9 ± 0.2	8	−0.24 ± 0.05^b	0.17 ± 0.02	−0.59 ± 0.04	1.5 ± 0.2	6
Tricine	0.010 ± 0.002	−15.6 ± 1.8	5.3 ± 0.2	12	−40.2 ± 1.2	8.2 ± 0.3	11	−0.38 ± 0.03	0.13 ± 0.01	−0.44 ± 0.02	1.2 ± 0.1	6
Zn 7 µM	0.005 ± 0.001	−2.4 ± 2.9^a	7.1 ± 0.1^a	4	−30.4 ± 1.8^a	9.8 ± 0.5	6	−0.14 ± 0.01^a	0.33 ± 0.08	−0.52 ± 0.06	2.4 ± 0.6	6
Cu 205 nM	0.007 ± 0.002	2.9 ± 1.2^a	7.4 ± 0.1^a	6	−26.8 ± 1.2^a	9.7 ± 0.8	6	−0.18 ± 0.04^a	0.21 ± 0.08	−0.48 ± 0.04	3.1 ± 0.4^b	6
H179E/H181A
HEPES	0.010 ± 0.002	−12.7 ± 1.0	5.0 ± 0.2	7	−42.9 ± 1.8	9.1 ± 0.4	6	−0.32 ± 0.04	0.14 ± 0.02	−0.59 ± 0.04	1.3 ± 0.1	5
Tricine	0.010 ± 0.001	−12.1 ± 1.1	5.2 ± 0.2	13	−41.2 ± 1.3	8.4 ± 0.2	8	−0.34 ± 0.03	0.14 ± 0.01	−0.55 ± 0.02	1.2 ± 0.1	5
Zn 7 µM	0.008 ± 0.002	−9.4 ± 0.9	6.0 ± 0.3	5	−36.0 ± 2.4	9.2 ± 0.8	4	−0.31 ± 0.04	0.23 ± 0.11	−0.65 ± 0.17	2.8 ± 1.1	3
Cu 205 nM	0.009 ± 0.002	−8.6 ± 1.9	5.6 ± 0.2	8	−37.2 ± 1.1	8.0 ± 0.4	6	−0.33 ± 0.02	0.20 ± 0.06	−0.64 ± 0.12	2.4 ± 0.7	5

Solution	Activation				Inactivation			Kinetics
Ca_v2.3 WT	P_Ca(max) ± SEM	V_1/2 ± SEM	k ± SEM	n	V_1/2 ± SEM	k ± SEM	n	A_f ± SEM	τ_f ± SEM	A_s ± SEM	τ_s ± SEM	n
HEPES	0.011 ± 0.003	1.5 ± 1.0a	7.2 ± 0.1a	8	−36.1 ± 1.1b	8.9 ± 0.2	8	−0.24 ± 0.05b	0.17 ± 0.02	−0.59 ± 0.04	1.5 ± 0.2	6
Tricine	0.010 ± 0.002	−15.6 ± 1.8	5.3 ± 0.2	12	−40.2 ± 1.2	8.2 ± 0.3	11	−0.38 ± 0.03	0.13 ± 0.01	−0.44 ± 0.02	1.2 ± 0.1	6
Zn 7 µM	0.005 ± 0.001	−2.4 ± 2.9a	7.1 ± 0.1a	4	−30.4 ± 1.8a	9.8 ± 0.5	6	−0.14 ± 0.01a	0.33 ± 0.08	−0.52 ± 0.06	2.4 ± 0.6	6
Cu 205 nM	0.007 ± 0.002	2.9 ± 1.2a	7.4 ± 0.1a	6	−26.8 ± 1.2a	9.7 ± 0.8	6	−0.18 ± 0.04a	0.21 ± 0.08	−0.48 ± 0.04	3.1 ± 0.4b	6
H179E/H181A
HEPES	0.010 ± 0.002	−12.7 ± 1.0	5.0 ± 0.2	7	−42.9 ± 1.8	9.1 ± 0.4	6	−0.32 ± 0.04	0.14 ± 0.02	−0.59 ± 0.04	1.3 ± 0.1	5
Tricine	0.010 ± 0.001	−12.1 ± 1.1	5.2 ± 0.2	13	−41.2 ± 1.3	8.4 ± 0.2	8	−0.34 ± 0.03	0.14 ± 0.01	−0.55 ± 0.02	1.2 ± 0.1	5
Zn 7 µM	0.008 ± 0.002	−9.4 ± 0.9	6.0 ± 0.3	5	−36.0 ± 2.4	9.2 ± 0.8	4	−0.31 ± 0.04	0.23 ± 0.11	−0.65 ± 0.17	2.8 ± 1.1	3
Cu 205 nM	0.009 ± 0.002	−8.6 ± 1.9	5.6 ± 0.2	8	−37.2 ± 1.1	8.0 ± 0.4	6	−0.33 ± 0.02	0.20 ± 0.06	−0.64 ± 0.12	2.4 ± 0.7	5

All parameters were calculated for each cell and then averaged. Statistically significant differences are noted with footnotes and are by one-way ANOVA, compared to the values obtained in Tricine:

P < 0.001.

P < 0.5.