TABLE V.

Effects of temperature on exponential components

Exp. 30°C 20°C 
 τa Areab τ Area 
 ms  ms  
EC1 0.26 ± 0.02 0.20 ± 0.03 0.23 ± 0.01 0.44 ± 0.02** 
EC2 1.11 ± 0.09 0.31 ± 0.03 1.13 ± 0.13 0.37 ± 0.02 
EC3 15.6 ± 2.99 0.19 ± 0.04 4.41 ± 1.05** 0.16 ± 0.02 
EC4 67.6 ± 17.5 0.25 ± 0.05 19.2 ± 4.12** 0.025 ± 0.006** 
EC5 735 ± 223 0.072 ± 0.042 2,428 ± 896* 0.00035 ± 0.00008** 
EO1 0.29 ± 0.03 0.77 ± 0.03 0.87 ± 0.08** 0.56 ± 0.04** 
EO2 0.77 ± 0.09 0.23 ± 0.03 2.27 ± 0.27** 0.44 ± 0.04** 
Exp. 30°C 20°C 
 τa Areab τ Area 
 ms  ms  
EC1 0.26 ± 0.02 0.20 ± 0.03 0.23 ± 0.01 0.44 ± 0.02** 
EC2 1.11 ± 0.09 0.31 ± 0.03 1.13 ± 0.13 0.37 ± 0.02 
EC3 15.6 ± 2.99 0.19 ± 0.04 4.41 ± 1.05** 0.16 ± 0.02 
EC4 67.6 ± 17.5 0.25 ± 0.05 19.2 ± 4.12** 0.025 ± 0.006** 
EC5 735 ± 223 0.072 ± 0.042 2,428 ± 896* 0.00035 ± 0.00008** 
EO1 0.29 ± 0.03 0.77 ± 0.03 0.87 ± 0.08** 0.56 ± 0.04** 
EO2 0.77 ± 0.09 0.23 ± 0.03 2.27 ± 0.27** 0.44 ± 0.04** 

All values are means ± SEM for two open and five closed exponential components (EO1–EO2 and EC1–EC5, respectively) fitted by the maximum likelihood method to dwell-time histograms from 8 (20°C; as in Table I, reproduced here again to facilitate comparison) and 13 (30°C) single-channel patches at 100 mV. The mean values have been compared using the Mann-Whitney test (*, P < 0.05; **, P < 0.01). These averaged values should not be used to calculate Po and mean open- and mean closed-time values. Rather, the separate values should be calculated for each channel and then averaged.

a

Mean dwell times for each exponential component.

b

Relative areas for each exponential component indicating its contribution to the total number of closed or open events.

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