TABLE I.

Effects of voltage on exponential components at 20°C

Exp. 40 mV 100 mV 
 τa Areab τ Area 
 ms  ms  
EC1 0.25 ± 0.02 0.35 ± 0.02 0.23 ± 0.01 0.44 ± 0.02** 
EC2 1.91 ± 0.26 0.36 ± 0.02 1.13 ± 0.13* 0.37 ± 0.02 
EC3 10.8 ± 2.47 0.24 ± 0.02 4.41 ± 1.05** 0.16 ± 0.02 
EC4 57.9 ± 17.0 0.055 ± 0.014 19.2 ± 4.12** 0.025 ± 0.006 
EC5 4,227 ± 2,067 0.0041 ± 0.0029 2,428 ± 896 0.00035 ± 0.00008* 
EO1 0.49 ± 0.06 0.58 ± 0.03 0.87 ± 0.08** 0.56 ± 0.04 
EO2 1.25 ± 0.21 0.42 ± 0.03 2.27 ± 0.27** 0.44 ± 0.04 
Exp. 40 mV 100 mV 
 τa Areab τ Area 
 ms  ms  
EC1 0.25 ± 0.02 0.35 ± 0.02 0.23 ± 0.01 0.44 ± 0.02** 
EC2 1.91 ± 0.26 0.36 ± 0.02 1.13 ± 0.13* 0.37 ± 0.02 
EC3 10.8 ± 2.47 0.24 ± 0.02 4.41 ± 1.05** 0.16 ± 0.02 
EC4 57.9 ± 17.0 0.055 ± 0.014 19.2 ± 4.12** 0.025 ± 0.006 
EC5 4,227 ± 2,067 0.0041 ± 0.0029 2,428 ± 896 0.00035 ± 0.00008* 
EO1 0.49 ± 0.06 0.58 ± 0.03 0.87 ± 0.08** 0.56 ± 0.04 
EO2 1.25 ± 0.21 0.42 ± 0.03 2.27 ± 0.27** 0.44 ± 0.04 

All values are means ± SEM for two open and five closed exponential components (EO1–EO2 and EC1–EC5, respectively) fitted by the maximum likelihood method to dwell-time histograms from eight single-channel patches at 40 and 100 mV and 20°C. The mean values have been compared using the Wilcoxon matched pairs test (*, P < 0.05; **, P < 0.01). These averaged values should not be used to calculate Po and mean open- and mean closed-time values. Rather, the separate values should be calculated for each channel and then averaged.

a

Mean dwell times for each exponential component.

b

Relative areas for each exponential component indicating its contribution to the total number of closed or open events.

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