Stiffness estimates from ΔL-F1 relationship and ΔL-FSS relationship
| Stiffness parameter | WT-cTnT | cTnTS199E/T204E | p-value |
| ED (mN mm−3) | 747.7 ± 40.9 | 522.9 ± 69.6 | 0.0175 |
| ER (mN mm−3) | 226.7 ± 15.7 | 114.3 ± 21.3 | 0.0019 |
| Stiffness parameter | WT-cTnT | cTnTS199E/T204E | p-value |
| ED (mN mm−3) | 747.7 ± 40.9 | 522.9 ± 69.6 | 0.0175 |
| ER (mN mm−3) | 226.7 ± 15.7 | 114.3 ± 21.3 | 0.0019 |
Muscle fiber bundles containing the cTnTS199E/T204E variant exhibited significantly lower stiffness parameters, ED and ER, when compared to bundles containing the WT-cTnT.