Effects of M1 receptor stimulation and PI 4 kinase inhibition on TRPV3 channel activity.
| Treatment: | CCh (+Ca2+i) | CCh (−Ca2+i) | PAO | |||||||
| DNA: | WT | R696A | K705A | WT | R696A | K705A | WT | R696A | K705A | |
| Fold change | Mean | 21.4 | 9.7 | 3.6a | 2.9 | 4.0 | 1.5a | 19.6 | 3.3a | 1.5a |
| SEM | 1.6 | 3.3 | 0.3 | 0.4 | 0.6 | 0.1 | 5.6 | 1.0 | 0.1 | |
| ΔV0.5 (mV) | Mean | −117.3 | −45.8a | −64.4a | −51.0 | −35.1 | −22.9 | −109.9 | −30.1a | −22.5a |
| SEM | 19.4 | 10.8 | 7.8 | 10.0 | 8.6 | 7.8 | 23.9 | 7.6 | 5.3 | |
| ΔVIF (%) | Mean | 23.1 | 21.3 | 8.3a | 5.5 | 24.0 | 1.3a | 19.1 | 12.2 | 2.3a |
| SEM | 3.5 | 5.2 | 3.0 | 1.2 | 8.6 | 1.2 | 3.4 | 3.8 | 1.0 | |
| n | 8 | 6 | 7 | 7 | 4 | 6 | 8 | 5 | 8 | |
| Treatment: | CCh (+Ca2+i) | CCh (−Ca2+i) | PAO | |||||||
| DNA: | WT | R696A | K705A | WT | R696A | K705A | WT | R696A | K705A | |
| Fold change | Mean | 21.4 | 9.7 | 3.6a | 2.9 | 4.0 | 1.5a | 19.6 | 3.3a | 1.5a |
| SEM | 1.6 | 3.3 | 0.3 | 0.4 | 0.6 | 0.1 | 5.6 | 1.0 | 0.1 | |
| ΔV0.5 (mV) | Mean | −117.3 | −45.8a | −64.4a | −51.0 | −35.1 | −22.9 | −109.9 | −30.1a | −22.5a |
| SEM | 19.4 | 10.8 | 7.8 | 10.0 | 8.6 | 7.8 | 23.9 | 7.6 | 5.3 | |
| ΔVIF (%) | Mean | 23.1 | 21.3 | 8.3a | 5.5 | 24.0 | 1.3a | 19.1 | 12.2 | 2.3a |
| SEM | 3.5 | 5.2 | 3.0 | 1.2 | 8.6 | 1.2 | 3.4 | 3.8 | 1.0 | |
| n | 8 | 6 | 7 | 7 | 4 | 6 | 8 | 5 | 8 | |
Effects of M1 stimulation (CCh) or PI 4 kinase inhibition (PAO) on current amplitude and voltage dependence for WT TRPV3, R696A, and K705A channels expressed in HM1 cells recorded with either 50 nM [Ca2+i] (+) or <10 nM [Ca2+i] (−) to control PLCβ activity. Currents were recorded in the presence of 10 µM (WT), 3 µM (R696A), or 6 µM (K705A) 2-APB at +80 mV. Final drug concentrations: 100 µM CCh and 30 µM PAO.
P < 0.05 comparing WT TRPV3 and R696A or K705A, respectively.