Estimated parameters from Scheme 1 fitted to α2 GlyR single-channel data
| Parameter name and unit | Scheme 1 |
| Rate constants ± CVM (%) | |
| α (s−1) | 330 ± 17% |
| β (s−1) | 120,000 ± 4% |
| d1+ (s−1) | 3,450 ± 53% |
| d1− (s−1) | 176 ± 71% |
| d2+ (s−1) | 3,860 ± 10% |
| d2− (s−1) | 2,740 ± 20% |
| k+1 (M−1s−1) | 18,200 ± 59% |
| k−1 (s−1) | 1,480 ± 49% |
| k+2 (M−1s−1) | 4.1 × 108 ± 75% |
| k−2 (s−1) | 6,680 ± 49% |
| Equilibrium constants ± CVM (%) | |
| E = β/α | 384 ± 13% |
| D1 = d1+/d1− | 28 ± 63% |
| D2 = d2+/d2− | 1.64 ± 36% |
| K1 = k−1/k+1 (μM) | 107 ± 73% |
| K2 = k−2/k+2 (μM) | 571 ± 97% |
| Popen curve parameters (±SDM) predicted from this scheme | |
| max Popen | 0.995 ± 0.001 |
| EC50 (μM) | 134 ± 55 |
| nH | 1.94 ± 0.03 |
| Parameter name and unit | Scheme 1 |
| Rate constants ± CVM (%) | |
| α (s−1) | 330 ± 17% |
| β (s−1) | 120,000 ± 4% |
| d1+ (s−1) | 3,450 ± 53% |
| d1− (s−1) | 176 ± 71% |
| d2+ (s−1) | 3,860 ± 10% |
| d2− (s−1) | 2,740 ± 20% |
| k+1 (M−1s−1) | 18,200 ± 59% |
| k−1 (s−1) | 1,480 ± 49% |
| k+2 (M−1s−1) | 4.1 × 108 ± 75% |
| k−2 (s−1) | 6,680 ± 49% |
| Equilibrium constants ± CVM (%) | |
| E = β/α | 384 ± 13% |
| D1 = d1+/d1− | 28 ± 63% |
| D2 = d2+/d2− | 1.64 ± 36% |
| K1 = k−1/k+1 (μM) | 107 ± 73% |
| K2 = k−2/k+2 (μM) | 571 ± 97% |
| Popen curve parameters (±SDM) predicted from this scheme | |
| max Popen | 0.995 ± 0.001 |
| EC50 (μM) | 134 ± 55 |
| nH | 1.94 ± 0.03 |
Rate constants are means of fits to three data sets ± CVM. The equilibrium constants (E, D1, D2, K1, and K2) are the means of the three values calculated for each separate set. Also listed are the average max Popen, EC50, and Hill slope calculated from the fitted rate constants (±SDM). These fits have 10 free parameters.