Effect of 10 mM lidocaine on the gating current properties of WT and mutant channels
| Channel | Control (TTX) | Lidocaine (+TTX) | Remaining gating charge | |||
| n | V1/2 | z | V1/2 | z | % | |
| WT | 6 | −63.8 ± 2.4 | 1.34 ± 0.02 | −63.3 ± 1.9 | 1.45 ± 0.03 | 79.0 ± 3.8 |
| R1135W | 4 | −65.9 ± 1.9 | 1.38 ± 0.06 | −63.8 ± 0.8 | 1.38 ± 0.06 | 91.4 ± 3.7 |
| V1142W | 4 | −50.4 ± 2.9 | 1.01 ± 0.06 | −57.6 ± 0.9 | 1.19 ± 0.03 | 90.0 ± 2.5 |
| A1145W | 3 | −56.0 ± 2.3 | 1.00 ± 0.03 | −54.3 ± 4.3 | 1.11 ± 0.03 | 96.2 ± 1.7 |
| I1284W | 3 | −57.3 ± 2.8 | 1.30 ± 0.11 | −54.2 ± 5.7 | 1.20 ± 0.00 | 100.0 ± 2.2 |
| I1287W | 3 | −55.0 ± 1.2 | 1.13 ± 0.03 | −59.2 ± 1.5 | 1.33 ± 0.01 | 92.0 ± 3.6 |
| K1295W | 4 | −53.7 ± 3.3 | 0.93 ± 0.01 | −59.2 ± 5.1 | 1.08 ± 0.02 | 91.2 ± 2.4 |
| Channel | Control (TTX) | Lidocaine (+TTX) | Remaining gating charge | |||
| n | V1/2 | z | V1/2 | z | % | |
| WT | 6 | −63.8 ± 2.4 | 1.34 ± 0.02 | −63.3 ± 1.9 | 1.45 ± 0.03 | 79.0 ± 3.8 |
| R1135W | 4 | −65.9 ± 1.9 | 1.38 ± 0.06 | −63.8 ± 0.8 | 1.38 ± 0.06 | 91.4 ± 3.7 |
| V1142W | 4 | −50.4 ± 2.9 | 1.01 ± 0.06 | −57.6 ± 0.9 | 1.19 ± 0.03 | 90.0 ± 2.5 |
| A1145W | 3 | −56.0 ± 2.3 | 1.00 ± 0.03 | −54.3 ± 4.3 | 1.11 ± 0.03 | 96.2 ± 1.7 |
| I1284W | 3 | −57.3 ± 2.8 | 1.30 ± 0.11 | −54.2 ± 5.7 | 1.20 ± 0.00 | 100.0 ± 2.2 |
| I1287W | 3 | −55.0 ± 1.2 | 1.13 ± 0.03 | −59.2 ± 1.5 | 1.33 ± 0.01 | 92.0 ± 3.6 |
| K1295W | 4 | −53.7 ± 3.3 | 0.93 ± 0.01 | −59.2 ± 5.1 | 1.08 ± 0.02 | 91.2 ± 2.4 |
Boltzmann parameters from a first-order fit of the Q-V relationships. All measurements were done in the presence of TTX. Data represent the mean ± SEM of at least three independent experiments (n).