Average properties of release flux
| Cell | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Pk | S | R(∞) | NFRC, initial | NFRC, final | n, cells | ||
| mM/s | mM/s | mM | |||||
| WT | AVG | 115 | 3.07 | 2.91 | 6.30 | 15.2 | 16 |
| SEM | 5.22 | 0.51 | 0.19 | 0.50 | 0.51 | ||
| dCasq-null | AVG | 86.0a | 2.76 | 2.34a | 16.2a | 5.13a | 19 |
| SEM | 12.5 | 0.25 | 0.17 | 3.4 | 1.07 |
| Cell | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Pk | S | R(∞) | NFRC, initial | NFRC, final | n, cells | ||
| mM/s | mM/s | mM | |||||
| WT | AVG | 115 | 3.07 | 2.91 | 6.30 | 15.2 | 16 |
| SEM | 5.22 | 0.51 | 0.19 | 0.50 | 0.51 | ||
| dCasq-null | AVG | 86.0a | 2.76 | 2.34a | 16.2a | 5.13a | 19 |
| SEM | 12.5 | 0.25 | 0.17 | 3.4 | 1.07 |
Two long duration pulses (one of 0.4 or 0.5 s, and the other of 1 s) to +30 mV were applied in every cell and the ensuing Ca2+ transients were imaged in line scan mode, respectively, at 2.5 or 5 ms per line. Column 2, peak flux, measured on the transient recorded at 2.5 ms/line and corrected for the blunting effect of low acquisition rate as described by Royer et al. (2008). Column 3, steady flux, reached at variable times into the 1-s pulse. Column 4, total amount of releasable calcium, defined as the asymptotic maximum of the time integral of net flux (compare with Fig. 7). Column 5, NFRC value reached immediately after the end of the initial peak of flux; for reference fibers, this was always the minimum value, while in null fibers it was usually the maximum. Column 6, NFRC value reached at the end of the interval where it is defined (∼250 ms); the NFRC value at this time was usually a maximum for reference fibers and a minimum for null fibers (compare with Fig. 3 B).
The value is significantly different from the corresponding value in the WT.