Table II.

Time-resolved fluorescence lifetime parameters

RCK1 WT D362/367A 
 −Ca2+ +Ca2+ −Ca2+ +Ca2+ 
τ1 (ns) 0.62 ± 0.040 0.70 ± 0.018 0.68 ± 0.096 0.71 ± 0.025 
τ2 (ns) 2.0 ± 0.068 1.6 ± 0.011 2.0 ± 0.14 1.9 ± 0.033 
τ3 (ns) 5.4 ± 0.049 5.1 ± 0.030 5.4 ± 0.10 5.4 ± 0.023 
α1 0.42 ± 0.032 0.29 ± 0.020 0.46 ± 0.054 0.45 ± 0.017 
α2 0.45 ± 0.026 0.64 ± 0.018 0.44 ± 0.052 0.45 ± 0.016 
α3 0.12 ± 0.0052 0.068 ± 0.0020 0.10 ± 0.0021 0.10 ± 0.0012 
f1 0.14 ± 0.021 0.13 ± 0.0088 0.18 ± 0.040 0.18 ± 0.013 
f2 0.49 ± 0.011 0.65 ± 0.0062 0.50 ± 0.033 0.50 ± 0.010 
f3 0.37 ± 0.010 0.22 ± 0.0027 0.32 ± 0.0073 0.31 ± 0.0028 
τavg (ns) 3.1 ± 0.010 2.3 ± 0.067 2.8 ± 0.049 2.8 ± 0.0037 
χ2 0.95 ± 0.0053 0.86 ± 0.0092 0.95 ± 0.018 0.95 ± 0.0069 
RCK1 WT D362/367A 
 −Ca2+ +Ca2+ −Ca2+ +Ca2+ 
τ1 (ns) 0.62 ± 0.040 0.70 ± 0.018 0.68 ± 0.096 0.71 ± 0.025 
τ2 (ns) 2.0 ± 0.068 1.6 ± 0.011 2.0 ± 0.14 1.9 ± 0.033 
τ3 (ns) 5.4 ± 0.049 5.1 ± 0.030 5.4 ± 0.10 5.4 ± 0.023 
α1 0.42 ± 0.032 0.29 ± 0.020 0.46 ± 0.054 0.45 ± 0.017 
α2 0.45 ± 0.026 0.64 ± 0.018 0.44 ± 0.052 0.45 ± 0.016 
α3 0.12 ± 0.0052 0.068 ± 0.0020 0.10 ± 0.0021 0.10 ± 0.0012 
f1 0.14 ± 0.021 0.13 ± 0.0088 0.18 ± 0.040 0.18 ± 0.013 
f2 0.49 ± 0.011 0.65 ± 0.0062 0.50 ± 0.033 0.50 ± 0.010 
f3 0.37 ± 0.010 0.22 ± 0.0027 0.32 ± 0.0073 0.31 ± 0.0028 
τavg (ns) 3.1 ± 0.010 2.3 ± 0.067 2.8 ± 0.049 2.8 ± 0.0037 
χ2 0.95 ± 0.0053 0.86 ± 0.0092 0.95 ± 0.018 0.95 ± 0.0069 

Triple-exponential fitting parameters of fluorescence lifetime. Lifetime fluorescence was recorded at λem = 330 nm (λex = 296 nm). Fractional preexponential factors αi correspond to lifetimes τi, respectively. fi is fractional contribution of each decay time, and τavg · χ2 values indicate goodness fit. Errors represent standard errors of the mean (n = 3).

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