Parameters of receptor-induced Ca2+ oscillation in the absence and presence of FSK
| Conditions | Peak [Ca2+]i (μM) | Duration (s) | Number of peaks | Period (s) | Percentage of oscillatory cells |
| 0.5 μM UTP | 1.5 ± 0.3 | 288 ± 38 | 10 ± 1 | 27 ± 1.1 | 100% (5/5) |
| 0.5 μM UTP + FSK | 1.7 ± 0.3 | 230 ± 14 | 7 ± 0.7 | 32 ± 1.4 | 100% (3/3) |
| 2 μM UTP | 1.9 ± 0.2 | 240 ± 20 | 12 ± 1 | 20 ± 1.2 | 88% (14/16) |
| 2 μM UTP + FSK | 2.0 ± 0.4 | 239 ± 41 | 11 ± 2 | 23 ± 1.3 | 75% (6/8) |
| 2 μM UTP + VIP | 2.7 ± 0.1 | 218 ± 34 | 10 ± 2 | 21 ± 2.2 | 82% (9/11) |
| 2 μM UTP + FSK + H-89 | 2.9 ± 0.4 | 193 ± 38 | 6 ± 1 | 31 ± 2.9 | 86% (6/7) |
| 2 μM UTP + FSK + Rp-8-Br-cAMPS | 1.8 ± 0.4 | 206 ± 60 | 10 ± 4 | 20 ± 1.5 | 75% (3/4) |
| 10 μM UTP | 2.6 ± 0.3 | 186 ± 38 | 11 ± 2 | 17 ± 0.8 | 89% (8/9) |
| 10 μM UTP + FSK | 2.4 ± 0.2 | 193 ± 35 | 11 ± 2 | 17 ± 0.6 | 45% (5/11) |
| 100 μM UTP | 3.3 ± 0.1 | 155 ± 36 | 8 ± 2 | 22 ± 1.6 | 57% (4/7) |
| 100 μM UTP + FSK | 3.5 ± 0.3 | 74 ± 7 | 4 ± 1 | 18 ± 3.3 | 38% (3/8) |
| 0.1 μM trypsin | 3.6 ± 0.7 | ||||
| 0.1 μM trypsin + FSK | 3.2 ± 0.3 |
| Conditions | Peak [Ca2+]i (μM) | Duration (s) | Number of peaks | Period (s) | Percentage of oscillatory cells |
| 0.5 μM UTP | 1.5 ± 0.3 | 288 ± 38 | 10 ± 1 | 27 ± 1.1 | 100% (5/5) |
| 0.5 μM UTP + FSK | 1.7 ± 0.3 | 230 ± 14 | 7 ± 0.7 | 32 ± 1.4 | 100% (3/3) |
| 2 μM UTP | 1.9 ± 0.2 | 240 ± 20 | 12 ± 1 | 20 ± 1.2 | 88% (14/16) |
| 2 μM UTP + FSK | 2.0 ± 0.4 | 239 ± 41 | 11 ± 2 | 23 ± 1.3 | 75% (6/8) |
| 2 μM UTP + VIP | 2.7 ± 0.1 | 218 ± 34 | 10 ± 2 | 21 ± 2.2 | 82% (9/11) |
| 2 μM UTP + FSK + H-89 | 2.9 ± 0.4 | 193 ± 38 | 6 ± 1 | 31 ± 2.9 | 86% (6/7) |
| 2 μM UTP + FSK + Rp-8-Br-cAMPS | 1.8 ± 0.4 | 206 ± 60 | 10 ± 4 | 20 ± 1.5 | 75% (3/4) |
| 10 μM UTP | 2.6 ± 0.3 | 186 ± 38 | 11 ± 2 | 17 ± 0.8 | 89% (8/9) |
| 10 μM UTP + FSK | 2.4 ± 0.2 | 193 ± 35 | 11 ± 2 | 17 ± 0.6 | 45% (5/11) |
| 100 μM UTP | 3.3 ± 0.1 | 155 ± 36 | 8 ± 2 | 22 ± 1.6 | 57% (4/7) |
| 100 μM UTP + FSK | 3.5 ± 0.3 | 74 ± 7 | 4 ± 1 | 18 ± 3.3 | 38% (3/8) |
| 0.1 μM trypsin | 3.6 ± 0.7 | ||||
| 0.1 μM trypsin + FSK | 3.2 ± 0.3 |
Duration, number of peaks, and period were defined as total time lapsed from the first to the final peaks, the total number of peaks, and their quotient. For these three parameters, only cells showing oscillations were included for analysis. The concentrations of FSK, VIP, H-89, and Rp-8-Br-cAMPS were 1 μM, 0.1 μM, 30 μM, and 1 mM, respectively. The [Ca2+]i did not oscillate with 0.1 μM trypsin in the absence or presence of FSK. Total number of cells for the experiments with trypsin with and without FSK was five and seven, respectively.