Gating current kinetics in the presence and absence of Mg2+
| Construct | τon, no Mg2+ | τon, + Mg2+ | n |
| ms | ms | ||
| I287D+F324D+W434F | 10.2 ± 0.8 | 13.4 ± 0.6a | 7 |
| I287D+W434F | 2.0 ± 0.2 | 2.4 ± 0.3 | 6 |
| W434F | 2.2 ± 0.1 | 2.3 ± 0.1 | 9 |
| Construct | τon, no Mg2+ | τon, + Mg2+ | n |
| ms | ms | ||
| I287D+F324D+W434F | 10.2 ± 0.8 | 13.4 ± 0.6a | 7 |
| I287D+W434F | 2.0 ± 0.2 | 2.4 ± 0.3 | 6 |
| W434F | 2.2 ± 0.1 | 2.3 ± 0.1 | 9 |
ON gating current traces recorded at −9 mV were fitted with a single exponential component to obtain values for τon, shown as mean ± SEM.
Significantly different from value obtained in the absence of Mg2+; P < 0.05 by ANOVA.