Table I. Steady-state gating current... | Rockefeller University Press

Table I.

Steady-state gating current parameters in the presence and absence of Mg²⁺

Construct	V₁		z₁		V₂		z₂		n
	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺
	mV				mV
I287D+F324D+W434F	−61 ± 1	−53 ± 1^a	1.4 ± 0.1	2.7 ± 0.5^a	−26 ± 1	−16 ± 1^a	3.0 ± 0.1	2.8 ± 0.4	7
I287D+W434F	−78 ± 2	−78 ± 1	2.2 ± 0.5	2.5 ± 0.3	−60 ± 1	−60 ± 0.2	3.1 ± 0.4	3.5 ± 0.3	6
W434F	−61 ± 2	−60 ± 2	2.8 ± 0.1	2.7 ± 0.1	−40 ± 1	−37 ± 1	5.6 ± 0.3	5.3 ± 0.2	8

Construct	V₁		z₁		V₂		z₂		n
	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺	no Mg²⁺	+Mg²⁺
	mV				mV
I287D+F324D+W434F	−61 ± 1	−53 ± 1^a	1.4 ± 0.1	2.7 ± 0.5^a	−26 ± 1	−16 ± 1^a	3.0 ± 0.1	2.8 ± 0.4	7
I287D+W434F	−78 ± 2	−78 ± 1	2.2 ± 0.5	2.5 ± 0.3	−60 ± 1	−60 ± 0.2	3.1 ± 0.4	3.5 ± 0.3	6
W434F	−61 ± 2	−60 ± 2	2.8 ± 0.1	2.7 ± 0.1	−40 ± 1	−37 ± 1	5.6 ± 0.3	5.3 ± 0.2	8

Normalized gating charge was plotted versus voltage, and data were fitted with the sum of two Boltzmann functions to obtain values for V_1/2 and valence (z). Parameters for q1 component of gating charge: V₁ and z₁. Parameters for q2 component of gating charge: V₂ and z₂. Values are provided as mean ± SEM.

Significantly different from value obtained in the absence of Mg²⁺; P < 0.05 by ANOVA.

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