The decay rate of the macroscopic current through the wild-type and different Y1618K mutant channels
| Wild-type | Y1618 single mutants | ||||||
| Y1618K | Y1618D | Y1618R | Y1618W | Y1618A | Y1618P | ||
| Decay rate(ms−1) | 0.25 ± 0.05 | 0.42 ± 0.02 | 0.78 ± 0.10 | 0.60 ± 0.10 | 0.23 ± 0.07 | 0.56 ± 0.01 | 0.28 ± 0.03 |
| Wild-type | Y1618 single mutants | ||||||
| Y1618K | Y1618D | Y1618R | Y1618W | Y1618A | Y1618P | ||
| Decay rate(ms−1) | 0.25 ± 0.05 | 0.42 ± 0.02 | 0.78 ± 0.10 | 0.60 ± 0.10 | 0.23 ± 0.07 | 0.56 ± 0.01 | 0.28 ± 0.03 |
| Y1618 double mutants | |||||
| Y1618K + F1619K | Y1618K + K1617A | Y1618K + E1616K | Y1618K + R1626C | Y1618K + F1651A | |
| Decay rate(ms−1) | 0.45 ± 0.01 | 0.36 ± 0.01 | 0.22 ± 0.02 | 0.21 ± 0.02 | 0.40 ± 0.03 |
| Y1618 double mutants | |||||
| Y1618K + F1619K | Y1618K + K1617A | Y1618K + E1616K | Y1618K + R1626C | Y1618K + F1651A | |
| Decay rate(ms−1) | 0.45 ± 0.01 | 0.36 ± 0.01 | 0.22 ± 0.02 | 0.21 ± 0.02 | 0.40 ± 0.03 |
The macroscopic current is elicited by a step depolarization to +10 mV from a holding potential of −120 mV. The decay phase of the elicited current is fitted with a monoexponential function, and the inverse of the current decay time constant is given in the table (n = 3–19).