TABLE I

Pore Helix, Selectivity Filter, and Extracellular Loop Residues Contributing >1 kcal/mole Static Field Energy to the Interaction of K+ along the Pore for Z < 0 Å

Pore helix, selectivity filter, and extracellular loops
KirBac1.1 I89 V105 E106 T107 L108 A109 T110 V111 D115 M116 P118 
Kir1.1 E111 L136 E137 T138 Q139 V140 T141 I142 F146 R147 E151 
Kir2.1 A115 I137 E138 T139 Q140 T141 T142 I143 F147 R148 D152 
Kir3.1 K114 I138 E139 T140 E141 A142 T143 I144 Y148 R149 D153 
Kir6.2 P102 I125 E126 V127 Q128 V129 T130 I131 G135 R136 E140 
Pore helix, selectivity filter, and extracellular loops
KirBac1.1 I89 V105 E106 T107 L108 A109 T110 V111 D115 M116 P118 
Kir1.1 E111 L136 E137 T138 Q139 V140 T141 I142 F146 R147 E151 
Kir2.1 A115 I137 E138 T139 Q140 T141 T142 I143 F147 R148 D152 
Kir3.1 K114 I138 E139 T140 E141 A142 T143 I144 Y148 R149 D153 
Kir6.2 P102 I125 E126 V127 Q128 V129 T130 I131 G135 R136 E140 

Residues are aligned across the five Kir channels to show the variation at each position between strongly contributing residues (bold) and non-electrostatic counterparts (italics). The yellow spheres in Fig. 1 A highlight the positions of these 11 sites.

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