TABLE III

Biophysical Properties of Individual Substitutions in hKv1.5

hKv1.5
K
G
L
Q
I
L
G
K
T
L
Q
A
S
M
R
E
 I  I  I  I  I  I  I  I   
position 418  420  422  424  426  428  430  432   
 (380)  (382)  (384)  (386)  (388)  (390)  (392)  (392)   
 Activation        Deactivation   Inactivation      
 V1/2 (mV)
 		  k (mV)
 		 τa (ms)
 		  τd (ms)
 		  V1/2,i (mV)
 		  ki (mV)
 		 n
 
K418T 1.5 ± 1.5   7.3 ± 0.6  4.5 ± 0.4   22.8 ± 3.0   −8.2 ± 0.7   4.9 ± 0.3  6 
I422S −26.4 ± 1.9   6.9 ± 0.6  12.6 ± 0.9   475 ± 27   −38.9 ± 1.0   4.5 ± 0.2  5 
I422A −24.1 ± 2.3   7.0 ± 0.7  5.7 ± 0.4   277 ± 52   −33.7 ± 1.7   5.8 ± 0.3  7 
I422W no current                 
I422R no current                 
K425E −10.1 ± 1.9   6.0 ± 0.3  4.3 ± 0.3   18.9 ± 3.2   −16.4 ± 1.4   3.6 ± 0.3  5 
K425F −5.2 ± 2.7   6.2 ± 1.3  7.8 ± 1.0   ND   ND   ND  3 
K425A 12.2 ± 1.6   7.0 ± 0.3  5.7 ± 0.8   19.2 ± 1.9   2.9 ± 1.5   5.5 ± 0.3  6 
T426A −1.5 ± 2.0   6.6 ± 0.3  3.6 ± 0.2   6.6 ± 0.8   −13.9 ± 2.6   4.5 ± 0.4  5 
T426W no current                 
T426R no current                 
Q428R −9.7 ± 1.4   6.5 ± 0.2  5.0 ± 0.8   22.0 ± 5.2   −19.3 ± 0.6   5.0 ± 0.1  5 
A429R −8.2 ± 1.8   7.1 ± 0.5  7.0 ± 0.6   4.4 ± 0.5   −16.2 ± 2.6   5.2 ± 0.3  5 
M431F −11.1 ± 1.4   5.9 ± 0.3  5.5 ± 0.9   16.1 ± 1.4   −16.3 ± 0.9   4.9 ± 0.3  6 
R432E −9.4 ± 0.6   4.9 ± 0.3  3.0 ± 0.3   13.7 ± 0.3   −14.0 ± 1.5   4.1 ± 0.2  4 
R432N −1.6 ± 1.2   5.8 ± 0.2  4.4 ± 0.5   57.0 ± 5.3   −7.5 ± 1.4   4.5 ± 0.2  8 
R432F 17.4 ± 1.3   7.0 ± 0.5  5.9 ± 0.7   12.5 ± 0.5   2.0 ± 1.1   4.3 ± 0.3  7 
V514I −5.1 ± 1.7   5.4 ± 0.7  4.5 ± 0.5   223 ± 19   −12.4 ± 1.4   3.6 ± 0.3  5 
Y521E −14.9 ± 1.4   5.3 ± 0.4  3.5 ± 0.3   11.9 ± 1.1   −22.8 ± 2.1   3.7 ± 0.4  5 
H524K −5.0 ± 1.6   4.6 ± 0.4  4.0 ± 0.4   26.6 ± 2.7   −11.5 ± 1.3   4.0 ± 0.1  5 
R525E −16.5 ± 0.9   5.6 ± 0.4  3.5 ± 0.2   25.8 ± 2.1   −26.4 ± 0.9   3.2 ± 0.1  6 
E526Q −9.6 ± 0.3   5.1 ± 0.3  4.8 ± 0.6   13.8 ± 1.8   −16.8 ± 1.2   4.5 ± 0.2  4 
T527K −7.1 ± 0.3   5.1 ± 0.2  4.2 ± 0.5   17.9 ± 3.2   −12.3 ± 1.4   3.8 ± 0.3  5 
D528R −10.9 ± 1.5   5.3 ± 0.5  4.7 ± 0.4   26.6 ± 2.6   −18.4 ± 0.6   4.0 ± 0.2  7 
H529Q −3.7 ± 1.2   5.0 ± 0.4  4.2 ± 0.5   18.8 ± 2.3   −9.0 ± 2.0   4.1 ± 0.4  6 
E531K −9.9 ± 2.8   5.6 ± 0.6  4.9 ± 0.5   20.4 ± 1.6   −17.0 ± 1.1   3.5 ± 0.2  5 
hKv1.5
K
G
L
Q
I
L
G
K
T
L
Q
A
S
M
R
E
 I  I  I  I  I  I  I  I   
position 418  420  422  424  426  428  430  432   
 (380)  (382)  (384)  (386)  (388)  (390)  (392)  (392)   
 Activation        Deactivation   Inactivation      
 V1/2 (mV)
 		  k (mV)
 		 τa (ms)
 		  τd (ms)
 		  V1/2,i (mV)
 		  ki (mV)
 		 n
 
K418T 1.5 ± 1.5   7.3 ± 0.6  4.5 ± 0.4   22.8 ± 3.0   −8.2 ± 0.7   4.9 ± 0.3  6 
I422S −26.4 ± 1.9   6.9 ± 0.6  12.6 ± 0.9   475 ± 27   −38.9 ± 1.0   4.5 ± 0.2  5 
I422A −24.1 ± 2.3   7.0 ± 0.7  5.7 ± 0.4   277 ± 52   −33.7 ± 1.7   5.8 ± 0.3  7 
I422W no current                 
I422R no current                 
K425E −10.1 ± 1.9   6.0 ± 0.3  4.3 ± 0.3   18.9 ± 3.2   −16.4 ± 1.4   3.6 ± 0.3  5 
K425F −5.2 ± 2.7   6.2 ± 1.3  7.8 ± 1.0   ND   ND   ND  3 
K425A 12.2 ± 1.6   7.0 ± 0.3  5.7 ± 0.8   19.2 ± 1.9   2.9 ± 1.5   5.5 ± 0.3  6 
T426A −1.5 ± 2.0   6.6 ± 0.3  3.6 ± 0.2   6.6 ± 0.8   −13.9 ± 2.6   4.5 ± 0.4  5 
T426W no current                 
T426R no current                 
Q428R −9.7 ± 1.4   6.5 ± 0.2  5.0 ± 0.8   22.0 ± 5.2   −19.3 ± 0.6   5.0 ± 0.1  5 
A429R −8.2 ± 1.8   7.1 ± 0.5  7.0 ± 0.6   4.4 ± 0.5   −16.2 ± 2.6   5.2 ± 0.3  5 
M431F −11.1 ± 1.4   5.9 ± 0.3  5.5 ± 0.9   16.1 ± 1.4   −16.3 ± 0.9   4.9 ± 0.3  6 
R432E −9.4 ± 0.6   4.9 ± 0.3  3.0 ± 0.3   13.7 ± 0.3   −14.0 ± 1.5   4.1 ± 0.2  4 
R432N −1.6 ± 1.2   5.8 ± 0.2  4.4 ± 0.5   57.0 ± 5.3   −7.5 ± 1.4   4.5 ± 0.2  8 
R432F 17.4 ± 1.3   7.0 ± 0.5  5.9 ± 0.7   12.5 ± 0.5   2.0 ± 1.1   4.3 ± 0.3  7 
V514I −5.1 ± 1.7   5.4 ± 0.7  4.5 ± 0.5   223 ± 19   −12.4 ± 1.4   3.6 ± 0.3  5 
Y521E −14.9 ± 1.4   5.3 ± 0.4  3.5 ± 0.3   11.9 ± 1.1   −22.8 ± 2.1   3.7 ± 0.4  5 
H524K −5.0 ± 1.6   4.6 ± 0.4  4.0 ± 0.4   26.6 ± 2.7   −11.5 ± 1.3   4.0 ± 0.1  5 
R525E −16.5 ± 0.9   5.6 ± 0.4  3.5 ± 0.2   25.8 ± 2.1   −26.4 ± 0.9   3.2 ± 0.1  6 
E526Q −9.6 ± 0.3   5.1 ± 0.3  4.8 ± 0.6   13.8 ± 1.8   −16.8 ± 1.2   4.5 ± 0.2  4 
T527K −7.1 ± 0.3   5.1 ± 0.2  4.2 ± 0.5   17.9 ± 3.2   −12.3 ± 1.4   3.8 ± 0.3  5 
D528R −10.9 ± 1.5   5.3 ± 0.5  4.7 ± 0.4   26.6 ± 2.6   −18.4 ± 0.6   4.0 ± 0.2  7 
H529Q −3.7 ± 1.2   5.0 ± 0.4  4.2 ± 0.5   18.8 ± 2.3   −9.0 ± 2.0   4.1 ± 0.4  6 
E531K −9.9 ± 2.8   5.6 ± 0.6  4.9 ± 0.5   20.4 ± 1.6   −17.0 ± 1.1   3.5 ± 0.2  5 

Sequence of L45 from hKv1.5 on top with Shaker numbering between parentheses. All mutations were done in an hKv1.5 background, and V1/2, k, and t were obtained as in Table I. ND, not determined.

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