Po of Kv7.2 and Kv7.3 WT and Mutant Channels at 0 mV
. | Cell-attached . | n . | Inside-out + 25 μM diC8-PIP2 . | n . |
|---|---|---|---|---|
| Kv7.2 wt | 0.18 ± 0.04 | 9 | 0.25 ± 0.04 | 5 |
| K452E | 0.10 ± 0.02 | 3 | 0.081 ± 0.031 | 3 |
| R459E | 0.10 ± 0.02 | 5 | 0.088 ± 0.019 | 4 |
| R461E | 0.093 ± 0.025 | 6 | 0.096 ± 0.008 | 3 |
| KRR-EEE | 0.017 ± 0.005 | 10 | 0.032 ± 0.020 | 8 |
| K425Q | 0.07 ± 0.01 | 4 | 0.05 ± 0.01 | 5 |
| K452Q | 0.086 ± 0.006 | 6 | 0.093 ± 0.014 | 5 |
| R459Q | 0.075 ± 0.007 | 4 | 0.061 ± 0.012 | 4 |
| R461Q | 0.076 ± 0.008 | 4 | 0.087 ± 0.015 | 4 |
| KRR-QQQ | 0.059 ± 0.013 | 6 | 0.055 ± 0.006 | 7 |
| R463Q | 0.045 ± 0.022 | 7 | 0.042 ± 0.019 | 8 |
| R467Q | 0.071 ± 0.033 | 10 | 0.079 ± 0.025 | 7 |
| R463E | 0.42 ± 0.20 | 10 | 0.46 ± 0.12 | 3 |
| R467E | 0.45 ± 0.19 | 8 | 0.52 ± 0.11 | 4 |
| K449E | 0.195 ± 0.07 | 8 | 0.19 ± 0.05 | 5 |
| K469E | 0.20 ± 0.06 | 4 | 0.21 ± 0.09 | 4 |
| D458N | 0.20 ± 0.04 | 4 | 0.21 ± 0.03 | 3 |
| Kv7.3 wt | 0.75 ± 0.09 | 4 | 0.72 ± 0.16 | 4 |
| K406Q | 0.26 ± 0.10 | 4 | 0.27 ± 0.07 | 4 |
| KKR-EEE | 0.34 ± 0.03 | 5 | 0.10 ± 0.04 | 5 |
| E423V | 0.65 ± 0.19 | 5 | 0.67 ± 0.17 | 5 |
| N431D | 0.73 ± 0.10 | 5 | 0.66 ± 0.16 | 4 |
. | Cell-attached . | n . | Inside-out + 25 μM diC8-PIP2 . | n . |
|---|---|---|---|---|
| Kv7.2 wt | 0.18 ± 0.04 | 9 | 0.25 ± 0.04 | 5 |
| K452E | 0.10 ± 0.02 | 3 | 0.081 ± 0.031 | 3 |
| R459E | 0.10 ± 0.02 | 5 | 0.088 ± 0.019 | 4 |
| R461E | 0.093 ± 0.025 | 6 | 0.096 ± 0.008 | 3 |
| KRR-EEE | 0.017 ± 0.005 | 10 | 0.032 ± 0.020 | 8 |
| K425Q | 0.07 ± 0.01 | 4 | 0.05 ± 0.01 | 5 |
| K452Q | 0.086 ± 0.006 | 6 | 0.093 ± 0.014 | 5 |
| R459Q | 0.075 ± 0.007 | 4 | 0.061 ± 0.012 | 4 |
| R461Q | 0.076 ± 0.008 | 4 | 0.087 ± 0.015 | 4 |
| KRR-QQQ | 0.059 ± 0.013 | 6 | 0.055 ± 0.006 | 7 |
| R463Q | 0.045 ± 0.022 | 7 | 0.042 ± 0.019 | 8 |
| R467Q | 0.071 ± 0.033 | 10 | 0.079 ± 0.025 | 7 |
| R463E | 0.42 ± 0.20 | 10 | 0.46 ± 0.12 | 3 |
| R467E | 0.45 ± 0.19 | 8 | 0.52 ± 0.11 | 4 |
| K449E | 0.195 ± 0.07 | 8 | 0.19 ± 0.05 | 5 |
| K469E | 0.20 ± 0.06 | 4 | 0.21 ± 0.09 | 4 |
| D458N | 0.20 ± 0.04 | 4 | 0.21 ± 0.03 | 3 |
| Kv7.3 wt | 0.75 ± 0.09 | 4 | 0.72 ± 0.16 | 4 |
| K406Q | 0.26 ± 0.10 | 4 | 0.27 ± 0.07 | 4 |
| KKR-EEE | 0.34 ± 0.03 | 5 | 0.10 ± 0.04 | 5 |
| E423V | 0.65 ± 0.19 | 5 | 0.67 ± 0.17 | 5 |
| N431D | 0.73 ± 0.10 | 5 | 0.66 ± 0.16 | 4 |
Given on the left are the values of the unitary current and Po of the indicated wt or mutant channels at 0 mV in cell-attached patches or in inside-out patches in the presence of 25 μM diC8-PIP2. n refers to the number of patches in each case. For Kv7.2, KRR-EEE and KRR-QQQ refer to the triple mutant of K452, R459, and R461. For Kv7.3, KKR-EEE refers to the triple mutant of K425, K432, and R434.