Table 2.

Primer sequences for real-time PCR

GeneForward/
Reverse		Sequence (5′–3′)Source
BTNL3 F 5′-TGC​CAC​AGT​ATC​GAG​GGA​GAA-3′ PrimerBank, 300388151c1 
R 3′-GCC​TCC​TCA​TCG​TAA​ATC​TGG-5′  
BTNL8 F 5′-CGG​GAG​CAT​ATC​CTG​TTC​CAT-3′ PrimerBank, 229577047c1 
R 3′-TAC​CCT​GGA​TTC​CAC​CTC​TCG-5′  
HNF4A F 5′-CGA​AGG​TCA​AGC​TAT​GAG​GAC​A-3′ PrimerBank, 71725338c2 
R 3′-ATC​TGC​GAT​GCT​GGC​AAT​CT-5′  
HNF4G F 5′-CAA​CGG​TGT​CAA​CTG​TCT​GTG-3′- PrimerBank, 115583653c2 
R 3′-AAA​CGT​GAC​TCT​TAC​GAA​TGC​T-5′  
Ang4 F 5′-TGG​CCA​GCT​TTG​GAA​TCA​CTG-3′ Brodziak et al. (2013)  
R 3′-GCT​TGG​CAT​CAT​AGT​GCT​GAC​G-5′  
B2m F 5′-ATG​GCT​CGC​TCG​GTG​ACC​CTG-3′ Guidry and Stroynowski (2005)  
R 3′-ATT​GCT​CAG​CTA​TCT​AGG​ATA-5′  
Btnl1 F 5′-TGA​CCA​GGA​GAA​ATC​GAA​GG-3′ Di Marco Barros et al. (2016)  
R 3′-CAC​CGA​GCA​GGA​CCA​ATA​GT-5′  
Btnl6 F 5′-GCA​CCT​CTC​TGG​TGA​AGG​AG-3′ Di Marco Barros et al. (2016)  
R 3′-ACC​GTC​TTC​TGG​ACC​TTT​GA-5′  
Cdh1 F 5′-TCC​TTG​TTC​GGA​TAT​GTG​TC-3′  
R 3′-GGC​ATG​CAC​CTA​AGA​ATC​AG-5′  
Clr-a/Clec2e F 5′-CAA​AGG​TTG​AAG​AGG​CTT​CC-3′ Zhang et al. (2012)  
R 3′-TCA​CGC​ATG​CTT​TGG​CAC​AT-5′  
Clr-f/Clec2h F 5′-TTG​AAA​CGA​GTT​CCA​TGG​GC-3′ Zhang et al. (2012)  
R 3′-GGT​CAT​AGA​GCA​TCT​GAT​TG-5′  
Gapdh F 5′-TGG​CAA​AGT​GGA​GAT​TGT​TGC​C-3′  
R 3′-AAG​ATG​GTG​ATG​GGC​TTC​CCG-5′  
H2-T3 F 5′-TTC​AAC​AGC​TCA​GGG​GAG​ACT​G-3′ Ohtsuka et al. (2008)  
R 3′-AAG​CTC​CGT​GTC​CTG​AAT​CAA​T-5′  
Hnf1a F 5′-CTT​CAT​GGC​AAC​CAT​GGC​CC-3′  
R 3′-GTC​TGA​GGT​GAA​GAC​CTG​CTT​G-5′  
Hnf4a F 5′-CAC​GCG​GAG​GTC​AAG​CTA​C-3′ PrimerBank, 6680239a1 
R 3′-CCC​AGA​GAT​GGG​AGA​GGT​GAT-5′  
Hnf4a (TNFΔARE data) F 5′-GTC​CAG​AGC​TAG​CGG​AGA​TG-3′  
R 3′-TAC​TGC​CGG​TCG​TTG​ATG​TA-5′  
Hnf4g F 5′-GTG​TCA​ACT​GTT​TAT​GTG​CCA​TC-3′ PrimerBank, 7305147a1 
R 3′-GTT​CAT​TTT​GCA​CCG​CTT​CTT​TT-5′  
Ifngr1 F 5′-CTG​GCA​GGA​TGA​TTC​TGC​TGG-3′ PrimerBank, 6754306a1 
R 3′-GCA​TAC​GAC​AGG​GTT​CAA​GTT​AT-5′  
Il1b F 5′-CGG​CAC​ACC​CAC​CCT​G-3′  
R 3′-AAA​CCG​TTT​TTC​CAT​CTT​CTT​CT-5′  
Il15 F 5′-ACA​TCC​ATC​TCG​TGC​TAC​TTG​T-3′ PrimerBank, 6680407a1 
R 3′-GCC​TCT​GTT​TTA​GGG​AGA​CCT-5′  
Il15ra F 5′-CGT​GTC​CAC​CTC​CCG​TAT​CTA-3′ PrimerBank, 29028296a1 
R 3′-AGA​CAT​ACC​TCT​CCC​TGG​AGT-5′  
Nos2 F 5′-GTT​CTC​AGC​CCA​ACA​ATA​CAA​GA-3′  
R 3′-GTG​GAC​GGG​TCG​ATG​TCA​C-5′  
Tnf F 5′-CAG​TTC​TAT​GGC​CCA​GAC​CCT-3′  
R 3′-CGG​ACT​CCG​CAA​AGT​CTA​AG-5′  
Reg3g F 5′-ATG​CTT​CCC​CGT​ATA​ACC​ATC​A-3′  
R 3′-GGC​CAT​ATC​TGC​ATC​ATA​CCA​G-5′  
ChIP-qPCR primers 
pBtnl1 F 5′-GTG​GCT​CAG​CCC​AGT​GCA​AAG​T-3′  
R 3′-GAC​ACA​CAA​TGC​TGC​CCC​CAG​G-5′  
pBtnl6 F 5′-GGC​ACA​GTG​TCC​ACC​ATC​AT-3′  
R 3′-GAA​CAT​ACG​CCC​AGG​TCA​CA-5′  
pHnf1a F 5′-CCT​GGC​ACA​GGA​GAA​CTT​TA-3′  
R 3′-AGT​CTG​ATG​TTG​GGC​TAG​GA-5′  
pBTNL3 F 5′-GGA​CAG​ATG​TTC​ACT​CTC​TCT​TG-3′  
R 3′-ACT​TGG​AGG​GAC​TTT​GTT​CTG-5′  
pBTNL8 F 5′-GGA​CAA​AGT​TCA​CCT​CCA​ATC​T-3′  
R 3′-GCT​GTC​ATG​TAC​CAG​AAG​TGT​AG-5′  
pHNF1A F 5′-AAG​TCC​AAA​GTT​CAG​TCC​CTT​C-3′  
R 3′-CCC​TGC​CTG​TTC​TGT​TTA​CAT-5′  
GeneForward/
Reverse		Sequence (5′–3′)Source
BTNL3 F 5′-TGC​CAC​AGT​ATC​GAG​GGA​GAA-3′ PrimerBank, 300388151c1 
R 3′-GCC​TCC​TCA​TCG​TAA​ATC​TGG-5′  
BTNL8 F 5′-CGG​GAG​CAT​ATC​CTG​TTC​CAT-3′ PrimerBank, 229577047c1 
R 3′-TAC​CCT​GGA​TTC​CAC​CTC​TCG-5′  
HNF4A F 5′-CGA​AGG​TCA​AGC​TAT​GAG​GAC​A-3′ PrimerBank, 71725338c2 
R 3′-ATC​TGC​GAT​GCT​GGC​AAT​CT-5′  
HNF4G F 5′-CAA​CGG​TGT​CAA​CTG​TCT​GTG-3′- PrimerBank, 115583653c2 
R 3′-AAA​CGT​GAC​TCT​TAC​GAA​TGC​T-5′  
Ang4 F 5′-TGG​CCA​GCT​TTG​GAA​TCA​CTG-3′ Brodziak et al. (2013)  
R 3′-GCT​TGG​CAT​CAT​AGT​GCT​GAC​G-5′  
B2m F 5′-ATG​GCT​CGC​TCG​GTG​ACC​CTG-3′ Guidry and Stroynowski (2005)  
R 3′-ATT​GCT​CAG​CTA​TCT​AGG​ATA-5′  
Btnl1 F 5′-TGA​CCA​GGA​GAA​ATC​GAA​GG-3′ Di Marco Barros et al. (2016)  
R 3′-CAC​CGA​GCA​GGA​CCA​ATA​GT-5′  
Btnl6 F 5′-GCA​CCT​CTC​TGG​TGA​AGG​AG-3′ Di Marco Barros et al. (2016)  
R 3′-ACC​GTC​TTC​TGG​ACC​TTT​GA-5′  
Cdh1 F 5′-TCC​TTG​TTC​GGA​TAT​GTG​TC-3′  
R 3′-GGC​ATG​CAC​CTA​AGA​ATC​AG-5′  
Clr-a/Clec2e F 5′-CAA​AGG​TTG​AAG​AGG​CTT​CC-3′ Zhang et al. (2012)  
R 3′-TCA​CGC​ATG​CTT​TGG​CAC​AT-5′  
Clr-f/Clec2h F 5′-TTG​AAA​CGA​GTT​CCA​TGG​GC-3′ Zhang et al. (2012)  
R 3′-GGT​CAT​AGA​GCA​TCT​GAT​TG-5′  
Gapdh F 5′-TGG​CAA​AGT​GGA​GAT​TGT​TGC​C-3′  
R 3′-AAG​ATG​GTG​ATG​GGC​TTC​CCG-5′  
H2-T3 F 5′-TTC​AAC​AGC​TCA​GGG​GAG​ACT​G-3′ Ohtsuka et al. (2008)  
R 3′-AAG​CTC​CGT​GTC​CTG​AAT​CAA​T-5′  
Hnf1a F 5′-CTT​CAT​GGC​AAC​CAT​GGC​CC-3′  
R 3′-GTC​TGA​GGT​GAA​GAC​CTG​CTT​G-5′  
Hnf4a F 5′-CAC​GCG​GAG​GTC​AAG​CTA​C-3′ PrimerBank, 6680239a1 
R 3′-CCC​AGA​GAT​GGG​AGA​GGT​GAT-5′  
Hnf4a (TNFΔARE data) F 5′-GTC​CAG​AGC​TAG​CGG​AGA​TG-3′  
R 3′-TAC​TGC​CGG​TCG​TTG​ATG​TA-5′  
Hnf4g F 5′-GTG​TCA​ACT​GTT​TAT​GTG​CCA​TC-3′ PrimerBank, 7305147a1 
R 3′-GTT​CAT​TTT​GCA​CCG​CTT​CTT​TT-5′  
Ifngr1 F 5′-CTG​GCA​GGA​TGA​TTC​TGC​TGG-3′ PrimerBank, 6754306a1 
R 3′-GCA​TAC​GAC​AGG​GTT​CAA​GTT​AT-5′  
Il1b F 5′-CGG​CAC​ACC​CAC​CCT​G-3′  
R 3′-AAA​CCG​TTT​TTC​CAT​CTT​CTT​CT-5′  
Il15 F 5′-ACA​TCC​ATC​TCG​TGC​TAC​TTG​T-3′ PrimerBank, 6680407a1 
R 3′-GCC​TCT​GTT​TTA​GGG​AGA​CCT-5′  
Il15ra F 5′-CGT​GTC​CAC​CTC​CCG​TAT​CTA-3′ PrimerBank, 29028296a1 
R 3′-AGA​CAT​ACC​TCT​CCC​TGG​AGT-5′  
Nos2 F 5′-GTT​CTC​AGC​CCA​ACA​ATA​CAA​GA-3′  
R 3′-GTG​GAC​GGG​TCG​ATG​TCA​C-5′  
Tnf F 5′-CAG​TTC​TAT​GGC​CCA​GAC​CCT-3′  
R 3′-CGG​ACT​CCG​CAA​AGT​CTA​AG-5′  
Reg3g F 5′-ATG​CTT​CCC​CGT​ATA​ACC​ATC​A-3′  
R 3′-GGC​CAT​ATC​TGC​ATC​ATA​CCA​G-5′  
ChIP-qPCR primers 
pBtnl1 F 5′-GTG​GCT​CAG​CCC​AGT​GCA​AAG​T-3′  
R 3′-GAC​ACA​CAA​TGC​TGC​CCC​CAG​G-5′  
pBtnl6 F 5′-GGC​ACA​GTG​TCC​ACC​ATC​AT-3′  
R 3′-GAA​CAT​ACG​CCC​AGG​TCA​CA-5′  
pHnf1a F 5′-CCT​GGC​ACA​GGA​GAA​CTT​TA-3′  
R 3′-AGT​CTG​ATG​TTG​GGC​TAG​GA-5′  
pBTNL3 F 5′-GGA​CAG​ATG​TTC​ACT​CTC​TCT​TG-3′  
R 3′-ACT​TGG​AGG​GAC​TTT​GTT​CTG-5′  
pBTNL8 F 5′-GGA​CAA​AGT​TCA​CCT​CCA​ATC​T-3′  
R 3′-GCT​GTC​ATG​TAC​CAG​AAG​TGT​AG-5′  
pHNF1A F 5′-AAG​TCC​AAA​GTT​CAG​TCC​CTT​C-3′  
R 3′-CCC​TGC​CTG​TTC​TGT​TTA​CAT-5′  
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