Table 2.
Optimal conditions for Cas9-RNP–mediated gene deletion in human myeloid cells
Monocyte-derived macrophage (M-CSF)Monocyte-derived DC (GM-CSF + IL-4)
Program CM-137 CM-137 
Buffer P3 P3 
Cell number range per reaction in 20 µl volume 0.5–5 × 106 fresh or thawed monocytes 0.5–5 × 106 fresh or thawed monocytes 
Culture conditions following electroporation Monocyte-derived macrophage culture medium containing M-CSF Mo-DC culture medium containing GM-CSF + IL-4 
Monocyte-derived macrophage (M-CSF)Monocyte-derived DC (GM-CSF + IL-4)
Program CM-137 CM-137 
Buffer P3 P3 
Cell number range per reaction in 20 µl volume 0.5–5 × 106 fresh or thawed monocytes 0.5–5 × 106 fresh or thawed monocytes 
Culture conditions following electroporation Monocyte-derived macrophage culture medium containing M-CSF Mo-DC culture medium containing GM-CSF + IL-4 
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