Table 1.
Optimal conditions for Cas9-RNP–mediated gene deletion in murine myeloid cells
MonocyteMacrophage (M-CSF)CD24+ DC (FLT3L)Sirpα+ DC (FLT3L)pDC (FLT3L)Macrophage (FLT3L)
Program CM-137 CM-137 CM-137 CM-137 EN-138 (crRNA, crRNAXT); CM-137 (sgRNA) CM-137 
Buffer P3 P3 P3 P3 P3 P3 
Cell number range per reaction in 20 µl volume 0.5–5 × 106 0.5–5 × 106 2–6 × 106 total BM cells 2–6 × 106 total BM cells 2–6 × 106 total BM cells 2–6 × 106 total BM cells 
Culture conditions following electroporation BMDM culture medium containing M-CSF BMDM culture medium containing M-CSF BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L 
MonocyteMacrophage (M-CSF)CD24+ DC (FLT3L)Sirpα+ DC (FLT3L)pDC (FLT3L)Macrophage (FLT3L)
Program CM-137 CM-137 CM-137 CM-137 EN-138 (crRNA, crRNAXT); CM-137 (sgRNA) CM-137 
Buffer P3 P3 P3 P3 P3 P3 
Cell number range per reaction in 20 µl volume 0.5–5 × 106 0.5–5 × 106 2–6 × 106 total BM cells 2–6 × 106 total BM cells 2–6 × 106 total BM cells 2–6 × 106 total BM cells 
Culture conditions following electroporation BMDM culture medium containing M-CSF BMDM culture medium containing M-CSF BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L BMDC culture medium containing FLT3L 
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