Table 1.
Sequence of Q-PCR primers
Gene nameGenBank accession no.Sequence (5′ to 3′)
DirectionFirst setSecond set
GAPDH NM_002046 Forward TGC​ACC​ACC​AAC​TGC​TTA​GC  
  Reverse GGC​ATG​GAC​TGT​GGT​CAT​GAG  
ACTB NM_001101 Forward ATG​TTT​GAG​ACC​TTC​AAC​AC  
  Reverse AGG​TAG​TCA​GTC​AGG​TCC​CGG​CC  
NUAK1 NM_014840 Forward AAG​GCA​CCT​ACG​GCA​AAG​TC GGG​AGC​TGT​ACG​ATT​ACA​TCA​G 
  Reverse GTC​TGA​TGT​GAA​CCA​TGT​CTT​GT ACA​CCG​TTC​TTG​TGA​CAA​TAG​TG 
NUAK2 NM_030952 Forward GAG​CGC​GAA​GCT​AGG​CAT​T CGC​CCA​AGC​CCC​TAA​TGA​AG 
  Reverse ATC​TCT​GGC​GAG​GCA​TAG​AGG TCC​CTC​CGT​ATG​TGC​ATC​AGA 
CKAP4 NM_006825 Forward CCG​TGG​AAT​CAC​TCC​AGA​AGG TGG​ACA​GTT​TGG​TTG​CAT​ACT​C 
  Reverse AGT​CCT​GAG​CAT​TTT​CAA​GTC​C CCT​CAG​GTC​ATC​TAG​TAA​ACC​CT 
COL7A1 NM_000094 Forward TTA​CGC​CGC​TGA​CAT​TGT​GTT GGC​TGC​AAT​TCT​CCA​TGT​GG 
  Reverse ACC​AGC​CCT​TCG​AGA​AAG​C CTG​TGA​GGC​AAC​TCG​CTT​CA 
CTGF NM_001901 Forward CAG​CAT​GGA​CGT​TCG​TCT​G ACC​GAC​TGG​AAG​ACA​CGT​TTG 
  Reverse AAC​CAC​GGT​TTG​GTC​CTT​GG CCA​GGT​CAG​CTT​CGC​AAG​G 
CYR61 NM_001554 Forward ACC​GCT​CTG​AAG​GGG​ATC​T GGT​CAA​AGT​TAC​CGG​GCA​GT 
  Reverse ACT​GAT​GTT​TAC​AGT​TGG​GCT​G GGA​GGC​ATC​GAA​TCC​CAG​C 
FMN2 NM_020066 Forward CAG​AGC​CTC​GGT​GTT​TTC​CAA  
  Reverse GCA​CAC​TCG​GTA​TCC​GAC​AG  
ABRA NM_139166 Forward AGC​AGT​GGG​CGA​ATG​AGA​AC AGG​TGT​CCA​AAA​CGG​TGG​TC 
  Reverse GTG​ATT​GGT​TTA​GGA​GCT​TGA​GG CAT​CCC​TCT​CGT​ACC​TGT​GG 
LATS1 NM_004690 Forward AAT​TTG​GGA​CGC​ATC​ATA​AAG​CC TTA​CCA​AGA​TCC​TCG​ACG​AGA​G 
  Reverse TCG​TCG​AGG​ATC​TTG​GTA​ACT​C CAC​ATT​CCC​TGG​TTT​CAT​GCT 
LATS2 NM_004691 Forward ACT​TTT​CCT​GCC​ACG​ACT​TAT​TC ACC​CCA​AAG​TTC​GGA​CCT​TAT 
  Reverse GAT​GGC​TGT​TTT​AAC​CCC​TCA CAT​TTG​CCG​GTT​CAC​TTC​TGC 
YAP NM_006106 Forward TAG​CCC​TGC​GTA​GCC​AGT​TA  
  Reverse TCA​TGC​TTA​GTC​CAC​TGT​CTG​T  
TAZ NM_181313 Forward CAC​CGT​GTC​CAA​TCA​CCA​GTC TGG​CAT​GTC​GGA​ATG​AAT​GAC 
  Reverse TCC​AAC​GCA​TCA​ACT​TCA​GGT GCTTCCCGACAGCACAGT 
TMSB4X NM_004202 Forward TGA​CAA​ACC​TGG​TAT​GGC​TGA  
  Reverse GCC​TCT​CCT​GTT​CGA​TAG​TTT​C  
CAPZA1 NM_006135 Forward GCC​GAC​TTC​GAT​GAT​CGT​GT TTC​AAT​GAC​GTT​CGG​CTA​CTA​C 
  Reverse AGT​AGC​CGA​ACG​TCA​TTG​AAT​AC GGC​GTG​AAC​TGA​TCC​ATG​TTA​T 
CAPZA2 NM_006136 Forward AAG​AGA​AGG​TGC​GTA​TAG​CAG​C CAT​TAC​CCG​AAT​GGA​GTC​TGC 
  Reverse AAG​CAG​TAA​CCG​AAC​ATC​ATT​GA ACT​TGA​GTG​GTT​GAA​GGA​GTG​A 
BCAM NM_005581 Forward GAG​GTG​CGC​TTG​TCT​GTA​CC GAA​TGG​TTC​CTT​ACC​GAC​CG 
  Reverse GCA​TAT​AAT​GGT​CGT​GGG​TTC​C TCG​TGC​ATT​GTG​ACC​TGG​AG 
TLN2 NM_015059 Forward GCG​TGT​CGA​GTC​ATT​CGG​G TGA​CCT​AAA​TTG​GCT​GGA​TCA​C 
  Reverse CCC​TTT​CCT​CGG​GTC​TTC​ATC AAC​TTC​CGT​CTA​AGC​AGC​AAC 
ARHGEF17 NM_014786 Forward CAG​TGA​CGA​CGA​CCG​AGA​C CCA​AGG​TGA​GCT​TTC​CCT​CG 
  Reverse TCC​TGA​TCC​AAT​AGG​GAG​CTT​C CCT​ACT​AGA​CCA​TAG​GTC​GTC​GT 
CDH1 NM_004360 Forward CGA​GAG​CTA​CAC​GTT​CAC​GG ATT​TTC​CCT​CGA​CAC​CCG​AT 
  Reverse GGG​TGT​CGA​GGG​AAA​AAT​AGG TCC​CAG​GCG​TAG​ACC​AAG​A 
ACTN2 NM_001103 Forward CAA​ACC​TGA​CCG​GGG​AAA​AAT ATG​GCC​TTG​GAC​TCT​GTG​C 
  Reverse CTG​AAT​AGC​AAA​GCG​AAG​GAT​GA GGT​GTT​CAC​GAT​GTC​TTC​AGC 
ACTN4 NM_004924 Forward GCA​GCA​TGG​GCG​ACT​ACA​T TGG​AGG​TCA​TAT​CAG​GGG​GAG​C 
  Reverse TTG​AGC​CCG​TCT​CGG​AAG​T GAG​ACC​AGC​TTG​ACG​CCT​TT 
ACTN1 NM_001130005 Forward TCC​ATC​GGA​GCC​GAA​GAA​ATC CCC​GAG​CTG​ATT​GAC​TAC​GG 
  Reverse GTG​TCG​GTG​GAT​CAA​AGC​ACA GCA​GTT​CCA​ACG​ATG​TCT​TCG 
Gene nameGenBank accession no.Sequence (5′ to 3′)
DirectionFirst setSecond set
GAPDH NM_002046 Forward TGC​ACC​ACC​AAC​TGC​TTA​GC  
  Reverse GGC​ATG​GAC​TGT​GGT​CAT​GAG  
ACTB NM_001101 Forward ATG​TTT​GAG​ACC​TTC​AAC​AC  
  Reverse AGG​TAG​TCA​GTC​AGG​TCC​CGG​CC  
NUAK1 NM_014840 Forward AAG​GCA​CCT​ACG​GCA​AAG​TC GGG​AGC​TGT​ACG​ATT​ACA​TCA​G 
  Reverse GTC​TGA​TGT​GAA​CCA​TGT​CTT​GT ACA​CCG​TTC​TTG​TGA​CAA​TAG​TG 
NUAK2 NM_030952 Forward GAG​CGC​GAA​GCT​AGG​CAT​T CGC​CCA​AGC​CCC​TAA​TGA​AG 
  Reverse ATC​TCT​GGC​GAG​GCA​TAG​AGG TCC​CTC​CGT​ATG​TGC​ATC​AGA 
CKAP4 NM_006825 Forward CCG​TGG​AAT​CAC​TCC​AGA​AGG TGG​ACA​GTT​TGG​TTG​CAT​ACT​C 
  Reverse AGT​CCT​GAG​CAT​TTT​CAA​GTC​C CCT​CAG​GTC​ATC​TAG​TAA​ACC​CT 
COL7A1 NM_000094 Forward TTA​CGC​CGC​TGA​CAT​TGT​GTT GGC​TGC​AAT​TCT​CCA​TGT​GG 
  Reverse ACC​AGC​CCT​TCG​AGA​AAG​C CTG​TGA​GGC​AAC​TCG​CTT​CA 
CTGF NM_001901 Forward CAG​CAT​GGA​CGT​TCG​TCT​G ACC​GAC​TGG​AAG​ACA​CGT​TTG 
  Reverse AAC​CAC​GGT​TTG​GTC​CTT​GG CCA​GGT​CAG​CTT​CGC​AAG​G 
CYR61 NM_001554 Forward ACC​GCT​CTG​AAG​GGG​ATC​T GGT​CAA​AGT​TAC​CGG​GCA​GT 
  Reverse ACT​GAT​GTT​TAC​AGT​TGG​GCT​G GGA​GGC​ATC​GAA​TCC​CAG​C 
FMN2 NM_020066 Forward CAG​AGC​CTC​GGT​GTT​TTC​CAA  
  Reverse GCA​CAC​TCG​GTA​TCC​GAC​AG  
ABRA NM_139166 Forward AGC​AGT​GGG​CGA​ATG​AGA​AC AGG​TGT​CCA​AAA​CGG​TGG​TC 
  Reverse GTG​ATT​GGT​TTA​GGA​GCT​TGA​GG CAT​CCC​TCT​CGT​ACC​TGT​GG 
LATS1 NM_004690 Forward AAT​TTG​GGA​CGC​ATC​ATA​AAG​CC TTA​CCA​AGA​TCC​TCG​ACG​AGA​G 
  Reverse TCG​TCG​AGG​ATC​TTG​GTA​ACT​C CAC​ATT​CCC​TGG​TTT​CAT​GCT 
LATS2 NM_004691 Forward ACT​TTT​CCT​GCC​ACG​ACT​TAT​TC ACC​CCA​AAG​TTC​GGA​CCT​TAT 
  Reverse GAT​GGC​TGT​TTT​AAC​CCC​TCA CAT​TTG​CCG​GTT​CAC​TTC​TGC 
YAP NM_006106 Forward TAG​CCC​TGC​GTA​GCC​AGT​TA  
  Reverse TCA​TGC​TTA​GTC​CAC​TGT​CTG​T  
TAZ NM_181313 Forward CAC​CGT​GTC​CAA​TCA​CCA​GTC TGG​CAT​GTC​GGA​ATG​AAT​GAC 
  Reverse TCC​AAC​GCA​TCA​ACT​TCA​GGT GCTTCCCGACAGCACAGT 
TMSB4X NM_004202 Forward TGA​CAA​ACC​TGG​TAT​GGC​TGA  
  Reverse GCC​TCT​CCT​GTT​CGA​TAG​TTT​C  
CAPZA1 NM_006135 Forward GCC​GAC​TTC​GAT​GAT​CGT​GT TTC​AAT​GAC​GTT​CGG​CTA​CTA​C 
  Reverse AGT​AGC​CGA​ACG​TCA​TTG​AAT​AC GGC​GTG​AAC​TGA​TCC​ATG​TTA​T 
CAPZA2 NM_006136 Forward AAG​AGA​AGG​TGC​GTA​TAG​CAG​C CAT​TAC​CCG​AAT​GGA​GTC​TGC 
  Reverse AAG​CAG​TAA​CCG​AAC​ATC​ATT​GA ACT​TGA​GTG​GTT​GAA​GGA​GTG​A 
BCAM NM_005581 Forward GAG​GTG​CGC​TTG​TCT​GTA​CC GAA​TGG​TTC​CTT​ACC​GAC​CG 
  Reverse GCA​TAT​AAT​GGT​CGT​GGG​TTC​C TCG​TGC​ATT​GTG​ACC​TGG​AG 
TLN2 NM_015059 Forward GCG​TGT​CGA​GTC​ATT​CGG​G TGA​CCT​AAA​TTG​GCT​GGA​TCA​C 
  Reverse CCC​TTT​CCT​CGG​GTC​TTC​ATC AAC​TTC​CGT​CTA​AGC​AGC​AAC 
ARHGEF17 NM_014786 Forward CAG​TGA​CGA​CGA​CCG​AGA​C CCA​AGG​TGA​GCT​TTC​CCT​CG 
  Reverse TCC​TGA​TCC​AAT​AGG​GAG​CTT​C CCT​ACT​AGA​CCA​TAG​GTC​GTC​GT 
CDH1 NM_004360 Forward CGA​GAG​CTA​CAC​GTT​CAC​GG ATT​TTC​CCT​CGA​CAC​CCG​AT 
  Reverse GGG​TGT​CGA​GGG​AAA​AAT​AGG TCC​CAG​GCG​TAG​ACC​AAG​A 
ACTN2 NM_001103 Forward CAA​ACC​TGA​CCG​GGG​AAA​AAT ATG​GCC​TTG​GAC​TCT​GTG​C 
  Reverse CTG​AAT​AGC​AAA​GCG​AAG​GAT​GA GGT​GTT​CAC​GAT​GTC​TTC​AGC 
ACTN4 NM_004924 Forward GCA​GCA​TGG​GCG​ACT​ACA​T TGG​AGG​TCA​TAT​CAG​GGG​GAG​C 
  Reverse TTG​AGC​CCG​TCT​CGG​AAG​T GAG​ACC​AGC​TTG​ACG​CCT​TT 
ACTN1 NM_001130005 Forward TCC​ATC​GGA​GCC​GAA​GAA​ATC CCC​GAG​CTG​ATT​GAC​TAC​GG 
  Reverse GTG​TCG​GTG​GAT​CAA​AGC​ACA GCA​GTT​CCA​ACG​ATG​TCT​TCG 
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