Antigens and dilutions
| Antigen . | Host/Target species . | Dilution . | Manufacturer . |
|---|---|---|---|
| Synapsin-1 | Rabbit anti-mouse | 1:200 | Abcam (ab64581) |
| GFAP | Rabbit anti-mouse | 1:300 | DSHB (8-1E7-s) |
| LAMP2 | Rat anti-mouse | 1:200 | DSHB (ABL-93-s) |
| HS (10E4 epitope) | Mouse anti-mouse | 1:200 | AMSBIO (F58-10E4) |
| NeuN | Rabbit anti-mouse | 1:250 | Millipore Sigma (MABN140) |
| CD68 | Rabbit polyclonal to CD68 | 1:200 | Abcam (ab125212) |
| GM2 | Mouse humanized | 1:400 | KM966 |
| LC3B | Rabbit anti-mouse | 1:200 | GeneTex (GTX82986) |
| VGLUT1 | Rabbit anti-mouse | 1:1,000 (cells) and 1:200 (tissues) | Abcam (ab104898) |
| PSD-95 | Mouse anti-mouse | 1:1,000 (cells) and 1:200 (tissues) | Abcam (ab99009) |
| Ubiquitin | Mouse | 1:200 | Abcam (ab7254) |
| β-Amyloid (D54D2) | Rabbit anti-mouse | 1:200 | Cell Signaling (8243S) |
| SCMAS | Rabbit anti-mouse | 1:200 | Abcam (ab181243) |
| Anti-O-GlcNAc | Mouse anti-mouse | 1:400 | Kerafast (EJH004) |
| MAP2 | Chicken anti-mouse | 1:2,000 | Abcam (ab5392) |
| Vesicular γ aminobutyric acid (GABA) transporter vGAT | Rabbit anti-mouse | 1:1,000 | Synaptic Systems (131003) |
| Gephyrin | Mouse anti-mouse | 1:1,000 | Synaptic Systems (147021) |
| Calreticulin | Rabbit anti-human | 1:200 | Sigma-Aldrich (208910) |
| IgG | Goat anti-rabbit, anti-mouse, anti-rat, or anti-chicken Alexa Fluor 488–, Alexa Fluor 555–, or Alexa Fluor 633–conjugated | 1:400 | Thermo Fisher Scientific |
| Antigen . | Host/Target species . | Dilution . | Manufacturer . |
|---|---|---|---|
| Synapsin-1 | Rabbit anti-mouse | 1:200 | Abcam (ab64581) |
| GFAP | Rabbit anti-mouse | 1:300 | DSHB (8-1E7-s) |
| LAMP2 | Rat anti-mouse | 1:200 | DSHB (ABL-93-s) |
| HS (10E4 epitope) | Mouse anti-mouse | 1:200 | AMSBIO (F58-10E4) |
| NeuN | Rabbit anti-mouse | 1:250 | Millipore Sigma (MABN140) |
| CD68 | Rabbit polyclonal to CD68 | 1:200 | Abcam (ab125212) |
| GM2 | Mouse humanized | 1:400 | KM966 |
| LC3B | Rabbit anti-mouse | 1:200 | GeneTex (GTX82986) |
| VGLUT1 | Rabbit anti-mouse | 1:1,000 (cells) and 1:200 (tissues) | Abcam (ab104898) |
| PSD-95 | Mouse anti-mouse | 1:1,000 (cells) and 1:200 (tissues) | Abcam (ab99009) |
| Ubiquitin | Mouse | 1:200 | Abcam (ab7254) |
| β-Amyloid (D54D2) | Rabbit anti-mouse | 1:200 | Cell Signaling (8243S) |
| SCMAS | Rabbit anti-mouse | 1:200 | Abcam (ab181243) |
| Anti-O-GlcNAc | Mouse anti-mouse | 1:400 | Kerafast (EJH004) |
| MAP2 | Chicken anti-mouse | 1:2,000 | Abcam (ab5392) |
| Vesicular γ aminobutyric acid (GABA) transporter vGAT | Rabbit anti-mouse | 1:1,000 | Synaptic Systems (131003) |
| Gephyrin | Mouse anti-mouse | 1:1,000 | Synaptic Systems (147021) |
| Calreticulin | Rabbit anti-human | 1:200 | Sigma-Aldrich (208910) |
| IgG | Goat anti-rabbit, anti-mouse, anti-rat, or anti-chicken Alexa Fluor 488–, Alexa Fluor 555–, or Alexa Fluor 633–conjugated | 1:400 | Thermo Fisher Scientific |