Table 1. Summary of evidence for/against... | Rockefeller University Press

Table 1.

Summary of evidence for/against EMT in metastasis

Gene of interest	Approach	Tumor type	Model	Result	Reference
Twist1	Conditional/inducible overexpression	SCC	DMBA	Sufficient for dissemination but must be down-regulated at metastatic site	Tsai et al. (2012)
Snai1	Inducible overexpression	BC	MMTV-Neu	Sufficient for dissemination but must be down-regulated at metastatic site	Tran et al. (2014)
Prrx1a/b	Inducible overexpression	PDAC	KPC	Isoform B is sufficient for dissemination but must switch with isoform A at metastatic site	Takano et al. (2016)
miR-200s	Conditional KO	PNET/PDAC	RT2/KPC	Sufficient to drive metastasis	Title et al. (2018)
Snai1	Conditional KO	BC	MMTV-PyMT	Critical for metastasis	Tran et al. (2014)
Snai1/Twist1	Conditional KO	PDAC	KPC	Dispensable for metastasis	Zheng et al. (2015)
Zeb1	Conditional KO	PDAC	KPC	Critical for metastasis	Krebs et al. (2017)
Fsp1	Lineage tracing and cell ablation	BC	MMTV-PyMT	Labeled cells contribute to and are required for metastasis	Xue et al. (2003)
Fsp1	Lineage tracing	BC	MMTV-PyMT	Labeled cells did not contribute to metastasis	Fischer et al. (2015)
Fsp1/αSMA	Lineage tracing	PDAC	KPF	Labeled cells did not contribute to metastasis	Chen et al. (2018)

Gene of interest	Approach	Tumor type	Model	Result	Reference
Twist1	Conditional/inducible overexpression	SCC	DMBA	Sufficient for dissemination but must be down-regulated at metastatic site	Tsai et al. (2012)
Snai1	Inducible overexpression	BC	MMTV-Neu	Sufficient for dissemination but must be down-regulated at metastatic site	Tran et al. (2014)
Prrx1a/b	Inducible overexpression	PDAC	KPC	Isoform B is sufficient for dissemination but must switch with isoform A at metastatic site	Takano et al. (2016)
miR-200s	Conditional KO	PNET/PDAC	RT2/KPC	Sufficient to drive metastasis	Title et al. (2018)
Snai1	Conditional KO	BC	MMTV-PyMT	Critical for metastasis	Tran et al. (2014)
Snai1/Twist1	Conditional KO	PDAC	KPC	Dispensable for metastasis	Zheng et al. (2015)
Zeb1	Conditional KO	PDAC	KPC	Critical for metastasis	Krebs et al. (2017)
Fsp1	Lineage tracing and cell ablation	BC	MMTV-PyMT	Labeled cells contribute to and are required for metastasis	Xue et al. (2003)
Fsp1	Lineage tracing	BC	MMTV-PyMT	Labeled cells did not contribute to metastasis	Fischer et al. (2015)
Fsp1/αSMA	Lineage tracing	PDAC	KPF	Labeled cells did not contribute to metastasis	Chen et al. (2018)

BC, breast cancer; DMBA, 7,12-dimethylbenz(a)anthracene; KPF, Kras^G12D/p53^FRT/+ or p53^R172H/+/Pdx1-Flp; Neu, rat Errb2 transgene; PNET, pancreatic neuroendocrine tumor; RT2, Rip-Tag2; SCC, squamous cell carcinoma.

[ViewLarge]