Table 1.
Ongoing approaches to induce immune tolerance
Type of approachModalityInstitutions supporting the concept
Clonal deletion using pre-apoptotic cells With autologous peripheral blood mononuclear cells; in vitro coupled to a cocktail of autoantigen-derived peptides prior to cell transfer Cellerys 
With autologous RBCs; in vitro coupled or loaded with autoantigens/autoantigen-peptides Rubius Therapeutics, SQZ Biotechnologies 
With autologous RBCs; in vivo targeted with RBC-binding molecules fused to autoantigens/autoantigen-peptides Anokion/Celgene, Kanyos (Anokion/Astellas) 
Therapeutic immunization With peptide or whole autoantigen proteins, alone or as cocktails, with or without adjuvants Apitope, Diamyd Medical, Immusant, Orban Biotech, UCB Pharma 
With DNA vaccines Tolerion 
With autoantigenic peptides containing thioredoxin motifs Imcyse 
Cell-based approaches Transferring autologous dendritic cells differentiated in vitro using cytokines, vitamin D3, dexamethasone, or genetically engineered to downregulate costimulatory molecules Baylor Research Institute, Diavacs, Leiden University 
Transferring in vitro inactivated autologous autoantigen-specific T cells to expose ergotypic antigens Opexa Therapeutics 
Transferring autologous regulatory chimeric antigen receptor-T (CAR–T reg) cells Txcell/Sangamo 
Administering engineered bacteria expressing host autoantigens together with host immune modulators ActoBio/Intrexon, Allero Therapeutics 
Engineered nanomedicines Delivering autoantigenic peptides/proteins, alone or in combination with immunomodulatory agents, to APCs using nanoparticle vehicles AntolRx/Pfizer, Cour Pharmaceuticals, Dendright/Janssen Biotech, Midatech Pharma, Regimmune, Selecta Biosciences, Toleranzia, Topas Therapeutics, Toralgen 
Directly targeting autoantigen-specific T cells with pMHC proteins coated onto nanoparticles, to reprogram and expand cognate T reg cells Parvus Therapeutics/Novartis 
Type of approachModalityInstitutions supporting the concept
Clonal deletion using pre-apoptotic cells With autologous peripheral blood mononuclear cells; in vitro coupled to a cocktail of autoantigen-derived peptides prior to cell transfer Cellerys 
With autologous RBCs; in vitro coupled or loaded with autoantigens/autoantigen-peptides Rubius Therapeutics, SQZ Biotechnologies 
With autologous RBCs; in vivo targeted with RBC-binding molecules fused to autoantigens/autoantigen-peptides Anokion/Celgene, Kanyos (Anokion/Astellas) 
Therapeutic immunization With peptide or whole autoantigen proteins, alone or as cocktails, with or without adjuvants Apitope, Diamyd Medical, Immusant, Orban Biotech, UCB Pharma 
With DNA vaccines Tolerion 
With autoantigenic peptides containing thioredoxin motifs Imcyse 
Cell-based approaches Transferring autologous dendritic cells differentiated in vitro using cytokines, vitamin D3, dexamethasone, or genetically engineered to downregulate costimulatory molecules Baylor Research Institute, Diavacs, Leiden University 
Transferring in vitro inactivated autologous autoantigen-specific T cells to expose ergotypic antigens Opexa Therapeutics 
Transferring autologous regulatory chimeric antigen receptor-T (CAR–T reg) cells Txcell/Sangamo 
Administering engineered bacteria expressing host autoantigens together with host immune modulators ActoBio/Intrexon, Allero Therapeutics 
Engineered nanomedicines Delivering autoantigenic peptides/proteins, alone or in combination with immunomodulatory agents, to APCs using nanoparticle vehicles AntolRx/Pfizer, Cour Pharmaceuticals, Dendright/Janssen Biotech, Midatech Pharma, Regimmune, Selecta Biosciences, Toleranzia, Topas Therapeutics, Toralgen 
Directly targeting autoantigen-specific T cells with pMHC proteins coated onto nanoparticles, to reprogram and expand cognate T reg cells Parvus Therapeutics/Novartis 

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