| Antibody . | Isotype . | KD, equilibrium . | CH . | BeH407 . | FSB0311 . | IQU2950 . | OBS2209 . | Uruma . | BeH343155 . | BeH506151 . | TRVL15537 . | BeH473130 . | BeH428890 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MAY-115 | IgG2a | n.b. | 2 (1–4) | 2 (1–3) | 5 (3–9) | 7 (4–14) | 9 (5–17) | 1 (1–3) | 166 (93–102) | 33 (14–67) | 32 (17–61) | 64 (36–115) | 92 (54–146) |
| MAY-116 | IgG1 | 20 ± 2 | 11 (9–16) | 37 (19–76) | 54 (23–105) | 11 (5–15) | 3 (1–5) | 11 (11–15) | 15 (8–32) | 65 (32–135) | 10 (6–20) | 23 (11–49) | 19 (4–96) |
| MAY-117 | IgG1 | 11 ± 3 | 5 (3–9) | 4 (2–9) | 5 (3–11) | 28 (16–51) | 3 (2–6) | 57 (26–113) | 8 (3–18) | 18 (10–33) | 60 (27–120) | 9 (6–17) | 39 (18–85) |
| MAY-118 | IgG1 | 13 ± 4 | 2 (1–3) | 6 (2–13) | 3 (2–5) | 24 (13–45) | 33 (16–71) | 48 (29–95) | 16 (7–34) | 8 (4–15) | 12 (10–33) | 36 (17–69) | 11 (7–18) |
| MAY-119 | IgG1 | 14 ± 4 | 2 (1–4) | 16 (9–31) | 1 (1–3) | 25 (14–55) | 2 (1–4) | 19 (10–36) | 8 (5–17) | 20 (13–39) | 50 (28–102) | 23 (10–46) | 25 (13–47) |
| MAY-120 | IgG1 | 18 ± 1 | 3 (1–6) | 10 (5–20) | 6 (3–11) | 5 (2–10) | 1 (1–2) | 41 (25–72) | 3 (1–7) | 68 (32–140) | 26 (12–55) | 13 (5–28) | 22 (14–36) |
| MAY-121 | IgG1 | 47 ± 13 | 4 (2–6) | 20 (11–36) | 7 (4–11) | 33 (23–49) | 3 (2–8) | 5 (4–7) | 280 (175–450) | 34 (23–49) | 34 (15–83) | 28 (19–50) | 45 (22–92) |
| MAY-122 | IgG2a | 4 ± 1 | 2 (1–6) | 3 (2–6) | 6 (3–10) | 11 (4–32) | 6 (3–13) | 5 (4–8) | 10 (6–16) | 7 (6–8) | 7 (3–13) | 5 (3–11) | 6 (4–10) |
| MAY-125 | IgG2a | 58 ± 19 | 5 (3–10) | 22 (14–42) | 5 (2–12) | 10 (6–19) | 10 (5–21) | 61 (28–122) | 30 (21–43) | 9 (3–17) | 103 (52–213) | 25 (17–58) | 35 (24–53) |
| MAY-130 | IgG1 | 3 ± 1 | 1 (1–2) | 5 (3–10) | 1 (1–2) | 4 (1–10) | 3 (1–10) | 2 (1–4) | 2 (1–3) | 25 (14–41) | 41 (27–62) | 5 (2–13) | 3 (2–4) |
| MAY-131 | IgG2a | n.b. | 7 (3–12) | 8 (2–14) | 3 (1–5) | 14 (11–17) | 12 (4–29) | 22 (15–30) | 64 (22–104) | 42 (36–47) | 22 (13–32) | 61 (45–78) | 43 (32–55) |
| MAY-133 | IgG2a | 243 ± 64 | 38 (14–106) | 25 (14–44) | 34 (25–32) | 50 (28–91) | 3 (2–5) | >10,000 | 31 (22–46) | 42 (30–57) | 1,911 (940–4,429) | 30 (18–51) | 39 (21–75) |
| MAY-134 | IgG2a | 152 ± 69 | 4 (2–7) | 9 (4–13) | 27 (19–39) | 50 (37–90) | 3 (2–4) | 17 (12–23) | 33 (21–53) | 34 (27–44) | 26 (18–38) | 41 (27–62) | 44 (22–90) |
| MAY-139 | IgG2a | 22 ± 11 | 36 (17–77) | 246 (133–342) | 22 (8–65) | 38 (15–103) | 3 (1–15) | 62 (38–105) | 27 (17–42) | 26 (16–42) | 33 (8–159) | 27 (15–29) | 39 (21–75) |
| MAY-140 | IgG2a | 170 ± 29 | 1,315 (408–6,040) | 18 (11–30) | >10,000 | 4 (2–8) | >10,000 | 69 (19–345) | 5 (2–6) | 3,788 (732–6,403) | 3 (1–15) | >10,000 | 2 (1–3) |
| MAY-146 | IgG1 | 18 ± 5 | 30 (26–34) | 63 (55–72) | 42 (37–47) | 22 (14–30) | 47 (22–89) | 33 (19–345) | 74 (65–83) | 29 (24–36) | 57 (30–84) | 85 (64–106) | 73 (65–82) |
| MAY-147 | IgG1 | 38 ± 8 | 23 (15–30) | 16 (11–22) | 98 (58–169) | 233 (96–630) | 11 (4–27) | 18 (9–25) | 88 (37–229) | 108 (28–151) | 105 (43–269) | 41 (22–77) | 112 (71–178) |
| MAY-151 | IgG1 | 29 ± 7 | 66 (48–55) | 52 (47–57) | 6 (2–18) | 59 (29–87) | 52 (42–83) | 27 (20–35) | 7 (2–13) | 78 (66–91) | 21 (17–26) | 8 (4–14) | 5 (1–11) |
| Antibody . | Isotype . | KD, equilibrium . | CH . | BeH407 . | FSB0311 . | IQU2950 . | OBS2209 . | Uruma . | BeH343155 . | BeH506151 . | TRVL15537 . | BeH473130 . | BeH428890 . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MAY-115 | IgG2a | n.b. | 2 (1–4) | 2 (1–3) | 5 (3–9) | 7 (4–14) | 9 (5–17) | 1 (1–3) | 166 (93–102) | 33 (14–67) | 32 (17–61) | 64 (36–115) | 92 (54–146) |
| MAY-116 | IgG1 | 20 ± 2 | 11 (9–16) | 37 (19–76) | 54 (23–105) | 11 (5–15) | 3 (1–5) | 11 (11–15) | 15 (8–32) | 65 (32–135) | 10 (6–20) | 23 (11–49) | 19 (4–96) |
| MAY-117 | IgG1 | 11 ± 3 | 5 (3–9) | 4 (2–9) | 5 (3–11) | 28 (16–51) | 3 (2–6) | 57 (26–113) | 8 (3–18) | 18 (10–33) | 60 (27–120) | 9 (6–17) | 39 (18–85) |
| MAY-118 | IgG1 | 13 ± 4 | 2 (1–3) | 6 (2–13) | 3 (2–5) | 24 (13–45) | 33 (16–71) | 48 (29–95) | 16 (7–34) | 8 (4–15) | 12 (10–33) | 36 (17–69) | 11 (7–18) |
| MAY-119 | IgG1 | 14 ± 4 | 2 (1–4) | 16 (9–31) | 1 (1–3) | 25 (14–55) | 2 (1–4) | 19 (10–36) | 8 (5–17) | 20 (13–39) | 50 (28–102) | 23 (10–46) | 25 (13–47) |
| MAY-120 | IgG1 | 18 ± 1 | 3 (1–6) | 10 (5–20) | 6 (3–11) | 5 (2–10) | 1 (1–2) | 41 (25–72) | 3 (1–7) | 68 (32–140) | 26 (12–55) | 13 (5–28) | 22 (14–36) |
| MAY-121 | IgG1 | 47 ± 13 | 4 (2–6) | 20 (11–36) | 7 (4–11) | 33 (23–49) | 3 (2–8) | 5 (4–7) | 280 (175–450) | 34 (23–49) | 34 (15–83) | 28 (19–50) | 45 (22–92) |
| MAY-122 | IgG2a | 4 ± 1 | 2 (1–6) | 3 (2–6) | 6 (3–10) | 11 (4–32) | 6 (3–13) | 5 (4–8) | 10 (6–16) | 7 (6–8) | 7 (3–13) | 5 (3–11) | 6 (4–10) |
| MAY-125 | IgG2a | 58 ± 19 | 5 (3–10) | 22 (14–42) | 5 (2–12) | 10 (6–19) | 10 (5–21) | 61 (28–122) | 30 (21–43) | 9 (3–17) | 103 (52–213) | 25 (17–58) | 35 (24–53) |
| MAY-130 | IgG1 | 3 ± 1 | 1 (1–2) | 5 (3–10) | 1 (1–2) | 4 (1–10) | 3 (1–10) | 2 (1–4) | 2 (1–3) | 25 (14–41) | 41 (27–62) | 5 (2–13) | 3 (2–4) |
| MAY-131 | IgG2a | n.b. | 7 (3–12) | 8 (2–14) | 3 (1–5) | 14 (11–17) | 12 (4–29) | 22 (15–30) | 64 (22–104) | 42 (36–47) | 22 (13–32) | 61 (45–78) | 43 (32–55) |
| MAY-133 | IgG2a | 243 ± 64 | 38 (14–106) | 25 (14–44) | 34 (25–32) | 50 (28–91) | 3 (2–5) | >10,000 | 31 (22–46) | 42 (30–57) | 1,911 (940–4,429) | 30 (18–51) | 39 (21–75) |
| MAY-134 | IgG2a | 152 ± 69 | 4 (2–7) | 9 (4–13) | 27 (19–39) | 50 (37–90) | 3 (2–4) | 17 (12–23) | 33 (21–53) | 34 (27–44) | 26 (18–38) | 41 (27–62) | 44 (22–90) |
| MAY-139 | IgG2a | 22 ± 11 | 36 (17–77) | 246 (133–342) | 22 (8–65) | 38 (15–103) | 3 (1–15) | 62 (38–105) | 27 (17–42) | 26 (16–42) | 33 (8–159) | 27 (15–29) | 39 (21–75) |
| MAY-140 | IgG2a | 170 ± 29 | 1,315 (408–6,040) | 18 (11–30) | >10,000 | 4 (2–8) | >10,000 | 69 (19–345) | 5 (2–6) | 3,788 (732–6,403) | 3 (1–15) | >10,000 | 2 (1–3) |
| MAY-146 | IgG1 | 18 ± 5 | 30 (26–34) | 63 (55–72) | 42 (37–47) | 22 (14–30) | 47 (22–89) | 33 (19–345) | 74 (65–83) | 29 (24–36) | 57 (30–84) | 85 (64–106) | 73 (65–82) |
| MAY-147 | IgG1 | 38 ± 8 | 23 (15–30) | 16 (11–22) | 98 (58–169) | 233 (96–630) | 11 (4–27) | 18 (9–25) | 88 (37–229) | 108 (28–151) | 105 (43–269) | 41 (22–77) | 112 (71–178) |
| MAY-151 | IgG1 | 29 ± 7 | 66 (48–55) | 52 (47–57) | 6 (2–18) | 59 (29–87) | 52 (42–83) | 27 (20–35) | 7 (2–13) | 78 (66–91) | 21 (17–26) | 8 (4–14) | 5 (1–11) |
IgG subclasses, KD, equilibrium values, and neutralization values of the 18 inhibitory anti-MAYV mAbs. IgG subclasses were determined by ELISA. KD, equilibrium values (nanomoles) were measured by BLI using recombinant MAYV E2 protein. EC50 values (ng/ml) along with 95% confidence intervals (in parentheses) were calculated from FRNT. Data are the mean and 95% confidence of two experiments performed in triplicate. Bold font indicates antibodies with elite neutralizing activity (<10 ng/ml) against the indicated MAYV strain. n.b., nonbinding.