| Reagent . | Source . | Description . |
|---|---|---|
| Antibodies | ||
| Anti-Aβ 33.1.1 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ Ab5 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ 2.1.3 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ 13.1.1 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-APP CT20 | Dr. Todd E. Golde | PMID: 19825975 |
| Total Tau | Dr. Benoit I. Giasson | PMID: 28760159 |
| Cd11b | Abcam | Cat # ab133357 |
| Iba-1 | Wako | Cat # 01919741 |
| GFAP | Cell Signaling | Cat # 3670 |
| GFAP | Dako | Cat # Z0334 |
| Anti-Aβ 82E1 | IBL | Cat # 10323 |
| PSD95 | Cell Signaling | Cat # 3409 |
| Spinophilin | Cell Signaling | Cat # 14136 |
| Synapsin 1 | Cell Signaling | Cat # 5297 |
| Synaptophysin | AbCam | Cat # ab32127 |
| Cleaved caspase 3 | Cell Signaling | Cat # 9661 |
| β-actin | Sigma | Cat # A5441 |
| V5 | Life Technologies | Cat # R96025 |
| FLAG | Sigma | Cat # F1804 |
| β-tubulin | Sigma | Cat # T2200 |
| HRP-labeled secondary antibodies (ImmPress) | Vector Biolabs | Cat # MP7401; MP7402 |
| Alexa Fluor–labeled secondary antibodies | Thermo Fisher | Cat # A-11001; A-11008 |
| Bacterial and virus strains | ||
| Not applicable | ||
| Biological samples | ||
| Adeno-associated viruses serotype 1 | Dr. Todd E. Golde | Made according to PMID: 23825679 |
| Chemicals, peptides, and recombinant proteins | ||
| Aβ42, Aβ40, fluorescent Aβ40 | Anaspec | Cat # AS-24224; AS-24236; AS-60492-01 |
| Flagellin | Invivogen | Cat # tlrl-epstfla |
| Critical commercial assays | ||
| Human IgG Fc biosensor | Fortebio, Pall Life Sciences | Not applicable |
| HEK-Blue Detection | Invivogen | Cat # hb-det3 |
| NanoString custom array | NanoString | PMID: 25619653 |
| LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | Thermo Fisher | Cat # L3224 |
| Deposited data | ||
| RNAseq data | https://www.synapse.org/#!Synapse:syn2580853/wiki/66722 | |
| Experimental models: cell lines | ||
| HEK-Blue hTLR5 cells | Invivogen | Not applicable |
| Chinese hamster ovary | ATCC | Not applicable |
| HEK 293 T | ATCC | Not applicable |
| Experimental models: organisms/strains | ||
| B6/C3H | Envigo | Not applicable |
| TgCRND8 | Dr. Todd E. Golde | Originally obtained from the Tanz Centre for Research in Neurodegenerative Diseases, Toronto, Canada |
| TLR5 KO | Jax Labs | Stock # 008377 |
| Oligonucleotides | ||
| Not applicable | ||
| Recombinant DNA | ||
| TLR and sTLR constructs (pAG3 or pAAV constructs) | Dr. Todd E. Golde | Fig. S2 |
| Software and algorithms | ||
| NanoStringNorm R package | NanoString | PMID: 22513995 |
| DIRAC | Dr. Nathan D. Price | PMID: 20523739 |
| Bioconductor package CQN | Dr. Nilüfer Ertekin-Taner | PMID: 22285995 |
| Corrplot R-package | Dr. Nilüfer Ertekin-Taner | https://github.com/taiyun/corrplot |
| Imagescope Pixel Count Program | Aperio/Leica Biosystems | Not applicable |
| Reagent . | Source . | Description . |
|---|---|---|
| Antibodies | ||
| Anti-Aβ 33.1.1 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ Ab5 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ 2.1.3 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-Aβ 13.1.1 | Dr. Todd E. Golde | PMID: 19825975 |
| Anti-APP CT20 | Dr. Todd E. Golde | PMID: 19825975 |
| Total Tau | Dr. Benoit I. Giasson | PMID: 28760159 |
| Cd11b | Abcam | Cat # ab133357 |
| Iba-1 | Wako | Cat # 01919741 |
| GFAP | Cell Signaling | Cat # 3670 |
| GFAP | Dako | Cat # Z0334 |
| Anti-Aβ 82E1 | IBL | Cat # 10323 |
| PSD95 | Cell Signaling | Cat # 3409 |
| Spinophilin | Cell Signaling | Cat # 14136 |
| Synapsin 1 | Cell Signaling | Cat # 5297 |
| Synaptophysin | AbCam | Cat # ab32127 |
| Cleaved caspase 3 | Cell Signaling | Cat # 9661 |
| β-actin | Sigma | Cat # A5441 |
| V5 | Life Technologies | Cat # R96025 |
| FLAG | Sigma | Cat # F1804 |
| β-tubulin | Sigma | Cat # T2200 |
| HRP-labeled secondary antibodies (ImmPress) | Vector Biolabs | Cat # MP7401; MP7402 |
| Alexa Fluor–labeled secondary antibodies | Thermo Fisher | Cat # A-11001; A-11008 |
| Bacterial and virus strains | ||
| Not applicable | ||
| Biological samples | ||
| Adeno-associated viruses serotype 1 | Dr. Todd E. Golde | Made according to PMID: 23825679 |
| Chemicals, peptides, and recombinant proteins | ||
| Aβ42, Aβ40, fluorescent Aβ40 | Anaspec | Cat # AS-24224; AS-24236; AS-60492-01 |
| Flagellin | Invivogen | Cat # tlrl-epstfla |
| Critical commercial assays | ||
| Human IgG Fc biosensor | Fortebio, Pall Life Sciences | Not applicable |
| HEK-Blue Detection | Invivogen | Cat # hb-det3 |
| NanoString custom array | NanoString | PMID: 25619653 |
| LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | Thermo Fisher | Cat # L3224 |
| Deposited data | ||
| RNAseq data | https://www.synapse.org/#!Synapse:syn2580853/wiki/66722 | |
| Experimental models: cell lines | ||
| HEK-Blue hTLR5 cells | Invivogen | Not applicable |
| Chinese hamster ovary | ATCC | Not applicable |
| HEK 293 T | ATCC | Not applicable |
| Experimental models: organisms/strains | ||
| B6/C3H | Envigo | Not applicable |
| TgCRND8 | Dr. Todd E. Golde | Originally obtained from the Tanz Centre for Research in Neurodegenerative Diseases, Toronto, Canada |
| TLR5 KO | Jax Labs | Stock # 008377 |
| Oligonucleotides | ||
| Not applicable | ||
| Recombinant DNA | ||
| TLR and sTLR constructs (pAG3 or pAAV constructs) | Dr. Todd E. Golde | Fig. S2 |
| Software and algorithms | ||
| NanoStringNorm R package | NanoString | PMID: 22513995 |
| DIRAC | Dr. Nathan D. Price | PMID: 20523739 |
| Bioconductor package CQN | Dr. Nilüfer Ertekin-Taner | PMID: 22285995 |
| Corrplot R-package | Dr. Nilüfer Ertekin-Taner | https://github.com/taiyun/corrplot |
| Imagescope Pixel Count Program | Aperio/Leica Biosystems | Not applicable |