| MHC I allele . | MHC I monomer folded with . | Template peptide exchanged for . | Efficiency of exchange . |
|---|---|---|---|
| % | |||
| H-2Kb | FAPGNAPAL | SIINFEKL | 105.5 ± 4.7 |
| FAPGNWPAL | 94.2 ± 10.8 | ||
| FAPGNYPAA | 84.4 ± 6.2 | ||
| FAPGNAPAL | 4.2 ± 0.1 | ||
| - | 0.1 ± 0.1 | ||
| SIINFEKL | - | 107.4 ± 12.6 | |
| HLA-A*02:01 | IAKEPVHGV | NLVPMVATV | 101.3 ± 13.2 |
| LLDQLIEEV | 86.0 ± 14.6 | ||
| GLCTLVAML | 70.7 ± 16.3 | ||
| IAKEPVHGV | 27.4 ± 2.7 | ||
| - | 7.2 ± 2.2 | ||
| NLVPMVATV | - | 80.5 ± 15.3 |
| MHC I allele . | MHC I monomer folded with . | Template peptide exchanged for . | Efficiency of exchange . |
|---|---|---|---|
| % | |||
| H-2Kb | FAPGNAPAL | SIINFEKL | 105.5 ± 4.7 |
| FAPGNWPAL | 94.2 ± 10.8 | ||
| FAPGNYPAA | 84.4 ± 6.2 | ||
| FAPGNAPAL | 4.2 ± 0.1 | ||
| - | 0.1 ± 0.1 | ||
| SIINFEKL | - | 107.4 ± 12.6 | |
| HLA-A*02:01 | IAKEPVHGV | NLVPMVATV | 101.3 ± 13.2 |
| LLDQLIEEV | 86.0 ± 14.6 | ||
| GLCTLVAML | 70.7 ± 16.3 | ||
| IAKEPVHGV | 27.4 ± 2.7 | ||
| - | 7.2 ± 2.2 | ||
| NLVPMVATV | - | 80.5 ± 15.3 |
Peptide exchange on MHC I was performed with 0.5 µM monomers (H-2Kb or HLA-A*02:01), incubated with 50 µM peptide as described in Materials and methods. Monomers were also incubated in the absence of peptide to determine the stability of the complexes under these conditions. To quantify the amount of eluted peptide, standard curves were created with the respective synthetic peptides. H-2Kb–SIINFEKL and HLA-A*02:01–NLVPMVATV were measured as positive controls.