Table 1. Summary of optimal CRISPR/Cas9... | Rockefeller University Press

Table 1.

Summary of optimal CRISPR/Cas9 gene KO conditions (CD4⁺ and CD8⁺ T cells)

Parameter	Mouse (CD4/CD8)		Human (CD4/CD8)
Parameter	Resting	Activated	Resting	Activated
Primary cell nucleofection solution	P4	P4	P2	P2
Pulse	DS137	CM137	EH100	EH100
Culture condition	Complete T cell media with IL-7 (preculture 24 h)	Complete T cell media with anti-CD3, anti-CD28, and IL-2	Complete T cell media	Complete T cell media with anti-CD3, anti-CD28, and IL-2
No. of cells per well	1–10 × 10⁶	1–10 × 10⁶	1–10 × 10⁶	1–10 × 10⁶

Parameter	Mouse (CD4/CD8)		Human (CD4/CD8)
Parameter	Resting	Activated	Resting	Activated
Primary cell nucleofection solution	P4	P4	P2	P2
Pulse	DS137	CM137	EH100	EH100
Culture condition	Complete T cell media with IL-7 (preculture 24 h)	Complete T cell media with anti-CD3, anti-CD28, and IL-2	Complete T cell media	Complete T cell media with anti-CD3, anti-CD28, and IL-2
No. of cells per well	1–10 × 10⁶	1–10 × 10⁶	1–10 × 10⁶	1–10 × 10⁶