Table 2. Characteristics of Dnah17 / ,... | Rockefeller University Press

Table 2.

Characteristics of Dnah17^+/+, Dnah17^+/M, and Dnah17^M/M male mice

Parameters	Dnah17^+/+	Dnah17^+/M	Dnah17^M/M
Body weight (g)	27.0 ± 0.5	26.1 ± 1.0^a	26.3 ± 0.8^a
Testis weight (mg)	164.5 ± 3.3	167.4 ± 0.4^a	176.2 ± 8.9^a
Testis/body weight ratio (10⁻³)	6.1 ± 0.1	6.4 ± 0.2^a	6.7 ± 0.2^a
Fertility
No. of fertile/total mice	3/3	3/3	5/5
Pups per fertile pair	24.7 ± 0.3	24.2 ± 0.2^a	8.7 ± 0.5***
Semen parameters
Sperm count (10⁷)	1.4 ± 0.1	1.4 ± 0.1^a	1.3 ± 0.1^a
Motile sperm (%)	76.7 ± 1.2	72.7 ± 0.9^a	19.8 ± 3.4***
Progressively motile sperm (%)	32.7 ± 0.3	35.0 ± 1.2^a	9.2 ± 2.0***
Sperm morphology
Morphologically normal (%)	88.9 ± 0.6	89.3 ± 0.7^a	90.4 ± 0.6^a
Abnormal head (%)	1.9 ± 0.6	3.4 ± 0.4^a	2.3 ± 1.0^a
Abnormal tail (%)	5.9 ± 0.6	4.0 ± 0.7^a	4.2 ± 0.7^a
Abnormal head and tail (%)	3.3 ± 0.7	3.3 ± 0.5^a	3.1 ± 0.2^a
Sperm flagella^b
Morphologically normal (%)	90.9 ± 0.5	92.7 ± 0.8^a	92.5 ± 0.6^a
Absent (%)	2.4 ± 0.4	1.9 ± 0.3^a	1.6 ± 0.3^a
Short (%)	1.1 ± 0.4	1.0 ± 0^a	1.1 ± 0.1^a
Coiled (%)	3.0 ± 0.9	2.4 ± 0.7^a	2.8 ± 0.6^a
Bent (%)	1.0 ± 0.3	1.0 ± 0.3^a	0.8 ± 0.1^a
Irregular caliber (%)	1.6 ± 0.1	1.0 ± 0.3^a	1.2 ± 0.3^a

Parameters	Dnah17^+/+	Dnah17^+/M	Dnah17^M/M
Body weight (g)	27.0 ± 0.5	26.1 ± 1.0^a	26.3 ± 0.8^a
Testis weight (mg)	164.5 ± 3.3	167.4 ± 0.4^a	176.2 ± 8.9^a
Testis/body weight ratio (10⁻³)	6.1 ± 0.1	6.4 ± 0.2^a	6.7 ± 0.2^a
Fertility
No. of fertile/total mice	3/3	3/3	5/5
Pups per fertile pair	24.7 ± 0.3	24.2 ± 0.2^a	8.7 ± 0.5***
Semen parameters
Sperm count (10⁷)	1.4 ± 0.1	1.4 ± 0.1^a	1.3 ± 0.1^a
Motile sperm (%)	76.7 ± 1.2	72.7 ± 0.9^a	19.8 ± 3.4***
Progressively motile sperm (%)	32.7 ± 0.3	35.0 ± 1.2^a	9.2 ± 2.0***
Sperm morphology
Morphologically normal (%)	88.9 ± 0.6	89.3 ± 0.7^a	90.4 ± 0.6^a
Abnormal head (%)	1.9 ± 0.6	3.4 ± 0.4^a	2.3 ± 1.0^a
Abnormal tail (%)	5.9 ± 0.6	4.0 ± 0.7^a	4.2 ± 0.7^a
Abnormal head and tail (%)	3.3 ± 0.7	3.3 ± 0.5^a	3.1 ± 0.2^a
Sperm flagella^b
Morphologically normal (%)	90.9 ± 0.5	92.7 ± 0.8^a	92.5 ± 0.6^a
Absent (%)	2.4 ± 0.4	1.9 ± 0.3^a	1.6 ± 0.3^a
Short (%)	1.1 ± 0.4	1.0 ± 0^a	1.1 ± 0.1^a
Coiled (%)	3.0 ± 0.9	2.4 ± 0.7^a	2.8 ± 0.6^a
Bent (%)	1.0 ± 0.3	1.0 ± 0.3^a	0.8 ± 0.1^a
Irregular caliber (%)	1.6 ± 0.1	1.0 ± 0.3^a	1.2 ± 0.3^a

For fertility test, three Dnah17^+/+, three Dnah17^+/M, and five Dnah17^M/M male mice (10 wk old) were each caged with two WT females (C57BL/6J; 10 wk old) for 90 d. For semen analysis, three 10-wk-old mice were examined for each genotype. Data are presented as mean ± SEM. ***P < 0.001; compared with the Dnah17^+/+ mice, one-way ANOVA with Dunnett‘s multiple comparison test.

NS, not significant.

Each spermatozoon was classified as only one type of flagellar morphology according to its major abnormality.

[ViewLarge]