Table 1.
Common astrocyte markers and reagents
Gene (protein)Labeled cells (CNS)Genetic linesAntibodiesNotesReference
Gfap (GFAP) Astrocytes + NPCs Fluorescent reporter, Cre, CreERT2 Upregulated in some reactive astrocytes Brenner et al., 1994; Zhuo et al., 2001; Su et al., 2004; Ganat et al., 2006; Liu et al., 2010  
Aldh1l1 (ALDH1L1) Astrocytes Fluorescent reporter, CreERT2 (new)  Cahoy et al., 2008; Srinivasan et al., 2016; Winchenbach et al., 2016  
Slc1a3 (GLAST) Astrocytes + NPCs Fluorescent reporter, CreERT Developed by Jeremy Nathans (Mouse Genome Informatics) Regan et al., 2007; Kang et al., 2010; de Melo et al., 2012; Wang et al., 2012  
Slc1a2 (GLT1) Astrocytes + NPCs Fluorescent reporter  Regan et al., 2007; Yang et al., 2011  
S100b (S100B) Astrocytes + OL lineage Fluorescent reporter, CreERT2  Zuo et al., 2004; McMahon et al., 2008; Harding et al., 2011  
Gjb6 (CX30) Astrocytes + NPCs CreERT2  Slezak et al., 2007; Srinivasan et al., 2016  
Slc6a11 (GAT3) Astrocytes + NPCs CreERT2  Srinivasan et al., 2016  
Nes (NESTIN) Astrocytes + NPCs Fluorescent reporter, Cre, CreERT2 Upregulated in some reactive astrocytes Betz et al., 1996; Tronche et al., 1999; Battiste et al., 2007; Lagace et al., 2007  
Vim (VIMENTIN) Astrocytes + NPCs Fluorescent reporter, LacZ Upregulated in some reactive astrocytes Colucci-Guyon et al., 1994  
C3 Astrocyte + certain Cx3cr1+ cells Fluorescent reporter (not finalized) Y (human) Upregulated in A1 reactive astrocytes; in situ hybridization required for murine tissue Liddelow et al., 2017  
Gene (protein)Labeled cells (CNS)Genetic linesAntibodiesNotesReference
Gfap (GFAP) Astrocytes + NPCs Fluorescent reporter, Cre, CreERT2 Upregulated in some reactive astrocytes Brenner et al., 1994; Zhuo et al., 2001; Su et al., 2004; Ganat et al., 2006; Liu et al., 2010  
Aldh1l1 (ALDH1L1) Astrocytes Fluorescent reporter, CreERT2 (new)  Cahoy et al., 2008; Srinivasan et al., 2016; Winchenbach et al., 2016  
Slc1a3 (GLAST) Astrocytes + NPCs Fluorescent reporter, CreERT Developed by Jeremy Nathans (Mouse Genome Informatics) Regan et al., 2007; Kang et al., 2010; de Melo et al., 2012; Wang et al., 2012  
Slc1a2 (GLT1) Astrocytes + NPCs Fluorescent reporter  Regan et al., 2007; Yang et al., 2011  
S100b (S100B) Astrocytes + OL lineage Fluorescent reporter, CreERT2  Zuo et al., 2004; McMahon et al., 2008; Harding et al., 2011  
Gjb6 (CX30) Astrocytes + NPCs CreERT2  Slezak et al., 2007; Srinivasan et al., 2016  
Slc6a11 (GAT3) Astrocytes + NPCs CreERT2  Srinivasan et al., 2016  
Nes (NESTIN) Astrocytes + NPCs Fluorescent reporter, Cre, CreERT2 Upregulated in some reactive astrocytes Betz et al., 1996; Tronche et al., 1999; Battiste et al., 2007; Lagace et al., 2007  
Vim (VIMENTIN) Astrocytes + NPCs Fluorescent reporter, LacZ Upregulated in some reactive astrocytes Colucci-Guyon et al., 1994  
C3 Astrocyte + certain Cx3cr1+ cells Fluorescent reporter (not finalized) Y (human) Upregulated in A1 reactive astrocytes; in situ hybridization required for murine tissue Liddelow et al., 2017  

OL, oligodendrocyte; Y, yes.

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